Morphological biomarkers in the Rhinocricus padbergi midgut exposed to contaminated soil

Souza, Tatiana da Silva; Fontanetti, Carmem Silvia

doi:10.1016/j.ecoenv.2010.09.009

Cited by 33 publications

(27 citation statements)

References 17 publications

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“…Previous studies showed the presence of morphological alterations in the midgut of R. padbergi after exposure to soil with similar characteristics to that evaluated in the present study (Souza and Fontanetti 2011). The intestinal epithelium of diplopods generally works as a barrier to the passage of contaminants from the lumen to the other parts of the body (Hopkin et al 1985).…”

Section: Discussionsupporting

confidence: 74%

“…The main function of fat body is the lipid, glycogen, protein, and uric acid storage (Hubert 1979;Hopkin and Read 1992;Fontanetti et al 2006). The histochemical results reported in the present research indicate that the perivisceral fat body may serve as a source of energy used in the detoxification of soil pollutants, a process previously observed in the midgut of R. padbergi (Souza and Fontanetti 2011).…”

Section: Discussionmentioning

confidence: 66%

“…One of the most remarkable sublethal endpoints applied to diplopods is histological and ultrastructural analysis of target tissues, particularly the midgut (Triebskorn et al 1991;Köhler and Triebskorn 1998;Godoy and Fontanetti 2010;Nogarol and Fontanetti 2010;Souza and Fontanetti 2011).…”

Section: Introductionmentioning

confidence: 99%

“…For this reason, they have gained increased attention from ecotoxicological research, and some species have been successfully used in ecotoxicological evaluations (Triebskorn et al 1991). Currently, the sensibility of Rhinocricus padbergi has been tested, yielding positive results (Godoy and Fontanetti 2010;Nogarol and Fontanetti 2010;Souza and Fontanetti 2011).…”

Section: Introductionmentioning

confidence: 99%

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Histological and Histochemical Analysis of the Fat Body of Rhinocricus padbergi (Diplopoda) Exposed to Contaminated Industrial Soil

Souza

Angelis

Fontanetti

2011

Water Air Soil Pollut

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Diplopods are components of the edaphic fauna, which makes them suitable bioindicators of soil quality. Some characteristics of the fat body of diplopods make it suitable for ecotoxicological studies. This organ, composed of diffuse tissue, fills the body cavity; it presents intense metabolic activity associated with lipid, glycogen, protein, and uric acid storage, being also responsible for storage, neutralization, and excretion of substances that are not useful to the organism. The present study aimed to investigate whether the perivisceral fat body of Rhinocricus padbergi can be used as a target organ in ecotoxicological studies and to identify possible histological and histochemical biomarkers in this organ. Upon examining the perivisceral fat body of individuals of R. padbergi exposed to different concentrations of industrial soil contaminated with polycyclic aromatic hydrocarbons (∑ 2,749.0 mg/kg) and metals (∑ 40,355.8 mg/kg), we have found evidence that this organ is sensitive to environmental pollutants present in the soil. The loss of integrity of the cell boundary associated with cytoplasmatic disorganization and depletion in total proteins, neutral polysaccharides, calcium, and lipids can be considered stress biomarkers for R. padbergi.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histological and Histochemical Analysis of the Fat Body of Rhinocricus padbergi (Diplopoda) Exposed to Contaminated Industrial Soil

Souza

Angelis

Fontanetti

2011

Water Air Soil Pollut

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“…The specie was chosen by its abundancy and for being considered a good toxicity bioindicator of soils (Godoy and Fontanetti, 2010;Nogarol and Fontanetti, 2010;Perez and Fontanetti, 2011;Souza and Fontanetti, 2011).…”

Section: Introductionmentioning

confidence: 99%

Sewage sludge toxicity in edaphic organism: Analysis of midgut responses in the diplopodRhinocricus padbergi

Bozzatto

Fontanetti

2012

Microscopy Res & Technique

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Diplopods are agile saprophagous invertebrates with the ability to colonize several soil layers. They feed on waste and decaying organic matter, making the soil in which they are present better aerated and mineral-enriched. Because of their habits, diplopods have been suggested to be used in studies to analyze possible toxic effects of impacted soils in edafic fauna. In that respect, this study aimed at checking the feasibility of using morphological analysis from millipede's midgut as a biomarker for soils exposed to sewage sludge from sewage treatment plants. Histological and histochemical techniques, applied to the species Rhinocricus padbergi's midgut, were used. Two sewage sludge samples were used; they were collected in two small towns in the countryside of São Paulo State. Both cities are part of the Piracicaba-Capivari-Jundiaí basin (PCJ-1 and PCJ-3). For 7 and 15-day periods, the animals were exposed to 10% concentrations and crude sludge for the PCJ-1sample and to 1, 10, and 50% concentrations and sludge for the PCJ-3 sample. The material exposed to the PCJ-1 sample showed an increase in the presence of cytoplasmic granules in both concentrations in all periods and a slight increase in the rate of epithelial renewal. The material of PCJ-3 sample presented the same alterations observed in PCJ-1 sample in higher frequence and over that high quantity of neutral polysaccharides in the hepatic cells layer. We can conclude that with the increase of sewage sludge concentrations tested, there is an increase in morphophysiologicals alterations frequencies found in the diplopods' midgut.

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Toxicity of trifluralin herbicide in a representative of the edaphic fauna: Histopathology of the midgut of Rhinocricus padbergi (Diplopoda)

Merlini

Nogarol

Marin-Morales

et al. 2012

Microscopy Res & Technique

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The aim of this study was to use the diplopod Rhinocricus padbergi as test organism to verify the possible toxic effects of the herbicide trifluralin in a representative of the edaphic fauna. Histological and histochemical techniques were applied in the midgut of the animals after exposure to different concentrations of trifluralin for 7 and 90 days (acute and subchronic exposure, respectively). The individuals from the control group did not present alterations to any of the exposures. In the acute exposure, the group exposed to a quarter and half of the field dose presented an accumulation of cytoplasmic granules in the hepatic cells; the field dose group presented an increase in the rate of epithelial renewal and the group exposed to double the field concentration presented an increase in the release of secretory vesicles. The subchronic response was obtained with the field dose and double field dose because the animals from a quarter and half groups did not survive until the end of the experiment. In both groups, the animals presented disruption of the epithelium and higher occurrence of hemocytes among the hepatic cells. The group exposed to double the field dose presented sites of cytoplasmic vacuolization, dilatation of the intercellular space, increase in the epithelial renewal, and release of secretory vesicles. The results alert for a careful use of this herbicide, since it promoted alterations in the midgut cells of the test organism used.

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Morphological biomarkers in the Rhinocricus padbergi midgut exposed to contaminated soil

Cited by 33 publications

References 17 publications

Histological and Histochemical Analysis of the Fat Body of Rhinocricus padbergi (Diplopoda) Exposed to Contaminated Industrial Soil

Histological and Histochemical Analysis of the Fat Body of Rhinocricus padbergi (Diplopoda) Exposed to Contaminated Industrial Soil

Sewage sludge toxicity in edaphic organism: Analysis of midgut responses in the diplopodRhinocricus padbergi

Toxicity of trifluralin herbicide in a representative of the edaphic fauna: Histopathology of the midgut of Rhinocricus padbergi (Diplopoda)

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