Morphological changes and self-incompatibility breakdown associated with autopolyploidization in Hylocereus species (Cactaceae)

Cohen, Hagai; Tel-Zur, Noemi

doi:10.1007/s10681-011-0536-5

Cited by 23 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The decrease in fruit weight and seed set in the autotetraploid and, more critically, in the autohexaploid, illustrated the disadvantages in plant fitness associated with the increase in ploidy levels in Hylocereus species. This conclusion is supported by the fact that the fruits obtained from induced autooctaploid (8 x ) H. megalanthus lines (obtained from germinating seeds) ceased developing at an early stage and aborted, resulting in almost zero fruit set [ 50 ]. The meaning of our findings is that enlarged cells (such as pollen or stomata) are a consequence of an increase in genome size (C-value) but that these changes are not systematically associated with the enlargement of other plant organs, as fruit weight or number of ovules.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

2013

Self Cite

View full text Add to dashboard Cite

BackgroundGenome doubling may have multi-level effects on the morphology, viability and physiology of polyploids compared to diploids. We studied the changes associated with autopolyploidization in two systems of somatic newly induced polyploids, diploid-autotetraploid and triploid-autohexaploid, belonging to the genus Hylocereus (Cactaceae). Stomata, fruits, seeds, embryos, and pollen were studied. Fruit pulp and seeds were subjected to metabolite profiling using established gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography (UPLC) Q-TOF-MS/MS (time of flight)-protocols.ResultsAutopolyploid lines produced lower numbers of tetrads, larger pollen grains with lower viability, larger stomata with lower density, and smaller fruits with lower seed numbers and decreased seed viability. The abundance of sugars was lower in the fruits and seeds of the two duplicated lines than in their donor lines, accompanied by increased contents of amino acids, tricarboxylic acid (TCA) cycle intermediates, organic acids and flavonoids. Betacyanins, the major fruit pigments in diploid and triploid donors, decreased following genome doubling. Both autopolyploid Hylocereus lines thus exhibited unfavorable changes, with the outcome being more dramatic in the autohexaploid than in the autotetraploid line.ConclusionInduced autotetraploid and autohexaploid lines exhibited morphological and cytological characteristics that differed from those of their donor plants and that were accompanied by significant metabolic alterations. It is suggested that a developmental arrest occurs in the fruits of the autohexaploid line, since their pericarp shows a greater abundance of acids and of reduced sugars. We conclude that genome doubling does not necessarily confer a fitness advantage and that the extent of alterations induced by autopolyploidization depends on the genetic background of the donor genotype.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Genome size was assessed by comparing the nuclear DNA content of the diploid H. monacanthus and the allotriploid S-75 with the corresponding autopolyploid lines D-27 and D-2.3, as described in Cohen and Tel-Zur [ 50 ]. Each line was analyzed at least four times to verify reproducibility.…”

Section: Methodsmentioning

confidence: 99%

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compared with the corresponding diploids, autopolyploids tend to have larger cells, which result in the enlargement of single organs, such as leaves, flowers and seeds [ 8 , 9 ]. Physiological traits such as plant height, growth rate, flowering time, and fertility also can be altered by polyploidization [ 10 - 12 ]. It has been shown that tetraploidization might significantly increase stress tolerance [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…A limited number of studies have investigated metabolic changes caused by autopolyploidization, and those studies focused on only specific metabolites [ 12 ]. The production of alkaloids was enhanced in artificial autotetraploids Hyoscyamus niger [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement

et al. 2015

View full text Add to dashboard Cite

BackgroundPolyploidy has often been considered to confer plants a better adaptation to environmental stresses. Tetraploid citrus rootstocks are expected to have stronger stress tolerance than diploid. Plenty of doubled diploid citrus plants were exploited from diploid species for citrus rootstock improvement. However, limited metabolic and molecular information related to tetraploidization is currently available at a systemic biological level. This study aimed to evaluate the occurrence and extent of metabolic and transcriptional changes induced by tetraploidization in Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka), which is a special citrus germplasm native to China and widely used as an iron deficiency tolerant citrus rootstock.ResultsDoubled diploid Ziyang xiangcheng has typical morphological and anatomical features such as shorter plant height, larger and thicker leaves, bigger stomata and lower stomatal density, compared to its diploid parent. GC-MS (Gas chromatography coupled to mass spectrometry) analysis revealed that tetraploidization has an activation effect on the accumulation of primary metabolites in leaves; many stress-related metabolites such as sucrose, proline and γ-aminobutyric acid (GABA) was remarkably up-regulated in doubled diploid. However, LC-QTOF-MS (Liquid chromatography quadrupole time-of-flight mass spectrometry) analysis demonstrated that tetraploidization has an inhibition effect on the accumulation of secondary metabolites in leaves; all the 33 flavones were down-regulated while all the 6 flavanones were up-regulated in 4x. By RNA-seq analysis, only 212 genes (0.8% of detected genes) are found significantly differentially expressed between 2x and 4x leaves. Notably, those genes were highly related to stress-response functions, including responses to salt stress, water and abscisic acid. Interestingly, the transcriptional divergence could not explain the metabolic changes, probably due to post-transcriptional regulation.ConclusionTaken together, tetraploidization induced considerable changes in leaf primary and secondary metabolite accumulation in Ziyang xiangcheng. However, the effect of tetraploidization on transcriptome is limited. Compared to diploid, higher expression level of stress related genes and higher content of stress related metabolites in doubled diploid could be beneficial for its stress tolerance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0450-4) contains supplementary material, which is available to authorized users.

show abstract

“…Efforts to get a hybrid clone that are self-compatible [SC] will be very beneficial to farmers so that farmers do not need to plant a variety of clones for the crossing [6] Cytoplasmic analysis gave the proof that hybrids are selfcompatible, and the hybrids are tetraploid. Thus polyploidy technique becomes important and gives prospective hope of solving their self-incompatibility in Hylocereusspp [7]. The quality and quantity of dragon fruit include sugar content, betalain pigment content and the overall condition of the fruit including weight and appearance thatareaffected by agronomic practices.…”

Section: Introductionmentioning

confidence: 99%

The Crossing Effect of Dragon Fruit Plant Caltivars [Hylocereus Sp.] on Yield

Subandi¹,

Mustari²,

Setian³

2018

IJET

View full text Add to dashboard Cite

Type of dragon fruit that is cultivated commercially is the red dragon fruit. There is no optimal production, because the flower of plant is incompatible. One way of improving production [fruiting] of dragon fruit is through manual crossing. This research aim was to study the crossing suitabilityof the red dragon fruit on fruiting. The research was conducted in dragon fruit plantation, West Java. Experimental design used was Randomized Block Design with four treatments and six replications. Treatments were: Natural Pollination [control];Crossing in one flower;Crossing between flowers; Crossing between varieties. Dimension of fruit measured were:diameter, length, fresh weight, and sugar content of fruit. Analysis of variance was applied with Duncan test at 5% significance level. Preparation of flower pollination was done by selecting the probable flower to bloom at night to come. The blooming flower can be predicted in the afternoon and the crossing was done at midnight.The result showed that the crossing between varieties was the best [improved fruiting]production. This report was suplemented with literature study on betalain showing that betalain is great beneficial for human health, and may be the next subject to study once the bulk production of fruit is done.

show abstract

Morphological changes and self-incompatibility breakdown associated with autopolyploidization in Hylocereus species (Cactaceae)

Cited by 23 publications

References 44 publications

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement

The Crossing Effect of Dragon Fruit Plant Caltivars [Hylocereus Sp.] on Yield

Contact Info

Product

Resources

About