Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterfly<i>Atrophaneura alcinous</i>Klug

Kubo-Irie, Miyoko; Irie, M.; Nakazawa, Tomoo; Mohri, Hideo

doi:10.1080/07924259.1998.9652660

Cited by 16 publications

(22 citation statements)

References 9 publications

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“…The apyrene sperm upper region has only a dense cap in some insects (Phillips 1970, Jamieson 1987, Kubo-Irie et al 1998, França & Báo 2000, including D. saccharalis and other lepidopterans (França & Báo 2000, Mancini & Dolder 2004a, Alves et al 2006. The apyrene flagella and the mitochondrial derivatives of D. saccharalis resemble those reported to other lepidopterans (Medeiros & Silveira 1996, KuboIrie et al 1998, Garvey et al 2000, Alves et al 2006.…”

Section: Discussionmentioning

confidence: 68%

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Pereira

Santos

2015

Neotrop Entomol

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In the present study, we describe the testis morphology and spermatogenesis of the sugarcane borer, one of the most destructive pests of sugarcane, using light, transmission, and scanning electron microscopies. Diatraea saccharalis (F.) larvae have paired, kidney-shaped testes that are limited externally and divided into four follicles by tunica cells. The adults have two fused whitish and spherical testes that are divided into eight follicles and are composed of spermatogonia, spermatocyte, spermatid, and sperm bundles. The spermatogonia and spermatocyte cysts are located at the apical extremity of the follicle, while the spermatid cysts and sperm bundles are located at the basal extremity of the follicle. Inside of the cysts, which are formed by the somatic cells, the germ line cells develop synchronously and are interconnected by cytoplasmic bridges. Each cyst contains only apyrene or eupyrene, and none of the cysts contain both of the cells. The inner part of the apyrene cyst consists of an electron-dense cap as opposed to a nucleus. The eupyrene sperm contains a nucleus, acrosome, and reticular and lacinate appendages. Both of the sperm types have a "9 + 9 + 2" axoneme and two mitochondrial derivatives at the tail region. The spermatogenesis process and spermatozoa morphology are similar to that of other Lepidoptera.

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Section: Discussionmentioning

confidence: 68%

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Pereira

Santos

2015

Neotrop Entomol

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“…It is well known that several species of animals produce atypical paraspermatozoa along with normal euspermatozoa during spermiogenesis, of which some examples are generally reviewed in Jamieson (1987a,b) and Jamieson et al (1999) and see also, e.g., Insecta, Sivinski (1984); Heteroptera, Schrader (1960); Lepidoptera, Silberglied et al (1984), Hamon and Chauvin (1992), Friedlander (1997), Yamashiki and Kawamura (1997), Kubo-Irie et al (1998); Symphyla, Dallai and Afzelius (2000); Gastropoda, Hodgson (1997); Archaeogastropoda, Nishiwaki (1964); Mesogastropoda, Ankel (1924), Healy and Jamieson (1981), Okura et al(1988), Afzelius et al (1989), Casse et al (1994). Both types of spermatozoa are released at mating, but paraspermatozoa, which derive from germinal cells with euspermatozoa synchronously, do not fertilize any eggs because their nuclei develop abnormally, whereas euspermatozoa with complete haploid genomes participate in fertilization (see, e.g., Sivinski, 1984;Jamieson, 1987a;Hodgson, 1997).…”

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confidence: 98%

Ultrastructural observations of eu‐ and paraspermiogenesis in the cottid fish Hemilepidotus gilberti (Teleostei: Scorpaeniformes: Cottidae)

Hayakawa¹,

Komaru²,

Munehara³

2002

Journal of Morphology

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The developmental process of eu- and paraspermatozoa in the cottid fish, Hemilepidotus gilberti, was observed by electron microscopy. Euspermatozoa of H. gilberti consist of a thin disk-like sperm head (about 3 microm in length), a short middle piece, and a long flagellum, but lack an acrosome. On the other hand, during spermiogenesis, aberrant spermatids, rich in cytoplasm and possessing binuclei, develop into cysts containing spermatids. The developing aberrant spermatids connect with normal spermatids and euspermatozoa by intercellular bridges. The early phase of chromatin condensation in aberrant spermatids is almost identical to that in normal spermatids, but the nuclei in the later phase develop into a mass of highly electron-dense globules. Since the aberrant spermatids complete karyokinesis but not cytokinesis at telophase of the second meiotic division, they are considered to develop into hyperpyrenic cells due to incomplete cytokinesis of the second meiotic division. These spermatids are oval in shape (5-7 microm in diameter) and lack a flagellum. The aberrant spermatids of H. gilberti are shed along with euspermatozoa and amount to about 50% of semen in volume. Judging from their form and developmental process, aberrant spermatids produced in H. gilberti are considered hyperpyrenic paraspermatozoa.

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“…The best-known case of sperm polymorphism occurs in butterflies and moths, which produce two distinct sperm types called apyrene and eupyrene spermatozoa (Riemann, 1970, Katsuno, 1977, Lai-Fook, 1982, Kubo-Irie et al, 1998, Jamieson et al, 1999, França and Báo, 2000, Mancini and Dolder, 2001, Mancini and Dolder, 2003, Mancini and Dolder, 2004aand Mancini and Dolder, 2004b. In general, eupyrene spermatozoa contain a nucleus and an acrosome, constituting an elongated head, and a long tail, whereas apyrene spermatozoa have a proximal tip covered by a dense cap and also a long tail.…”

Section: Introductionmentioning

confidence: 99%

“…However, the most evident difference is the elaborate extracellular structures present in eupyrene spermatozoa, which undergo morphological modifications along the male and female reproductive tract (Riemann, 1970, Phillips, 1971, Riemann and Thorson, 1971, Friedländer and Gitay, 1972, Lai-Fook, 1982, Kubo-Irie et al, 1998and Mancini and Dolder, 2003. In the testis, they possess two exclusive appendages, called the lacinate and reticular appendages (Phillips, 1970, Phillips, 1971and Jamieson et al, 1999.…”

Section: Introductionmentioning

confidence: 99%

“…In the testis, they possess two exclusive appendages, called the lacinate and reticular appendages (Phillips, 1970, Phillips, 1971and Jamieson et al, 1999. In the extra testicular regions, the eupyrene sperm lose their lacinate appendages and acquire a complex coat (Phillips, 1971, Riemann and Thorson, 1971, Lai-Fook, 1982, Kubo-Irie et al, 1998, Mancini and Dolder, 2001and Mancini and Dolder, 2003. The apyrene spermatozoa, however, present a less complex extracellular coat, acquired only in the extra testicular portions of the reproductive tract (Phillips, 1971, Friedländer and Gitay, 1972, Kubo-Irie et al, 1998, Garvey et al, 2000, Mancini and Dolder, 2001and Mancini and Dolder, 2003.…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical localization of tubulins in spermatids and spermatozoa of Euptoieta hegesia (Lepidoptera: Nymphalidae)

et al. 2005

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A comparative analysis of the distribution of tubulin types in apyrene and eupyrene sperm of Euptoieta hegesia butterflies was carried out, also verifying the presence of tubulin in lacinate appendages of the eupyrene sperm. Ultrathin sections of LR White embedded spermatids and spermatozoa were labeled for alpha, beta, gamma, alpha-acetylated and alpha-tyrosinated tubulins. Apyrene and eupyrene spermatids show the same antibody recognition pattern for tubulins. All tubulin types were detected in axonemal microtubules. Alpha and gamma tubulins were also detected on the cytoplasmic microtubules. However, for beta and tyrosinated tubulins only scattered labeling was detected on cytoplasmic microtubules and acetylated tubulin was not detected. In apyrene and eupyrene spermatozoa only the axoneme labeling was analyzed since cytoplasmic microtubules no longer exist in these cells. Alpha, beta and tyrosinated tubulins were easily detected on the apyrene and eupyrene axoneme; gamma tubulin was strongly marked on eupyrene axonemes but was scattered on the apyrene ones. Acetylated tubulin appeared with scattered labeling on the axoneme of both sperm types. Our results demonstrate significant differences in tubulin distribution in apyrene and eupyrene axonemal and cytoplasmic microtubules. Extracellular structures, especially the lacinate appendages, were not labeled by antibodies for any tubulin.

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Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterflyAtrophaneura alcinousKlug

Cited by 16 publications

References 9 publications

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Ultrastructural observations of eu‐ and paraspermiogenesis in the cottid fish Hemilepidotus gilberti (Teleostei: Scorpaeniformes: Cottidae)

Immunocytochemical localization of tubulins in spermatids and spermatozoa of Euptoieta hegesia (Lepidoptera: Nymphalidae)

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