Morphological Changes in Mesenteric Lymph Nodes and Lymphocyte Subpopulation Composition in Experimental Ulcerative Colitis

Abstract

Morphological changes in the mesenteric lymph nodes of male C57Bl/6 mice and subpopulation composition of lymphocytes in these nodes were studied in experimental acute and chronic ulcerative colitis induced by sodium dextran sulfate. Acute and chronic ulcerative colitis was associated with the development of reactive changes in the mesenteric lymph nodes. These changes were of mixed type and were characterized by follicular hyperplasia and sinus reaction. The content of CD19(+) B cells in the mesenteric lymph nodes decreased in acute ulcerative colitis, while the content of CD3(+)CD8(+) cytotoxic T cells increased, which presumably reflected activation of Th1 reactions. The increase in the count of CD4(+)CD25(+)FOXP3(+) regulatory T cells and CD3(+)CD8(+) cytotoxic T cells was due to intensive migration of lymphocytes from the thymus and the colonic compartment of the local immune system. Chronic ulcerative colitis was associated with higher levels of CD19(+) B cells and CD3(+)CD4(+) T helper cells in the mesenteric lymph nodes, which was characteristic of adoptive immunity reactions and chronization of the inflammatory process.

Garidisan: Improving the Quality of Ulcer Healing in Rats with Ulcerative Colitis

“…CD3 + CD8 + T cells specifically and directly kill target cells. Flow cytometry showed that the ratio of CD3 + CD4 + T lymphocytes and CD3 + CD8 + T lymphocytes in the UC model group was higher than normal controls, which was consistent with the findings of Postovalova's group [ 32 , 33 ]. In contrast, different doses of Garidisan, SASP, and Bupiyichangwan reduced the ratio of CD3 + CD4 + T lymphocytes and CD3 + CD8 + T lymphocytes in peripheral blood of UC rats.…”

Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathway

“…We have previously reported the increase of colonic CD8 + and CD25 + T cells in the DSS model of colitis related to disease worsening and increased histological damage [51], whereas both cell types were attenuated by TUS in the current study. We also observed an increase of CD4 + T cells and B220 + B cells in the MLN that may indicate a transition to a chronic state of the disease [52]. However, the upregulation of B220 + B cells in the spleen and MLN may contribute to gut homeostasis and attenuation of colitis while interacting with T regulatory cells [53].…”

The Therapeutic Effect of Changyuning Granules Combined With Retention Enema on Alleviating the Symptoms of Ulcerative Colitis

“…It is pointed out that in some studies the onset of UC during the active period is related to the body's immunity [22]. Changes in the number and ratio of T lymphocytes subsets can reflect the body's cellular immune function [23]. T lymphocytes in patients with UC during the active period are mainly manifested as the imbalance of subsets under antigen stimulation, leading to the abnormalities of other inflammatory mediators, cytokines and inflammatory cells, thus causing intestinal mucosal inflammation and tissue damage [24,25].…”