Morphological changes of porcine islets of Langerhans after collagenase and HBSS infusion of the pancreas

Abstract

Morphological changes of islets are observed after infusion of pancreata with collagenase and HBSS, most likely caused by volume expansion due to fluid entering the islets.

Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets

“…Although similar comparative experiments have not been performed in the pig pancreas, the findings of Lakey et al may be particularly relevant for the soft and fragile porcine pancreatic tissue. At 180 mm Hg of perfusion pressure the perfusion medium penetrates islets and expandes the intercellullar space thereby loosening islet structure and rendering pig islets more sensitive toward disintegration [61].…”

Xenotransplantation literature update: November–December, 2010

“…Hilling et al [5] studied the changes in porcine islet morphology after infusing harvested pancreata with collagenase. Pig pancreata were intraductally injected for 10 min at a continuous controlled pressure of 180 mmHg with either collagenase or Hank's balanced salt solution.…”