Morphological characterisation of Vitis vinifera L. seeds by image analysis and comparison with archaeological remains

Identification of inter- and intra-species variation in cereal grains through geometric morphometric analysis, and its resilience under experimental charring

“…The manner in which this is achieved depends on whether there are many features present that can be landmarked, or 100 whether curves, outlines and surfaces are the shapes' main homologous features. Recently, application of geometric morphometrics to archaeobotanical material has proven helpful to aid species identification (García-Granero et al, 2016) and, beyond this, to examine variation within species (Burger et al, 2011;Newton et al, 2006;Orrù et al, 2013;Pagnoux et al, 2014;Ros et al, 2014;Terral et al, 2012Terral et al, , 2010Terral et al, , 2004Ucchesu et al, 2016). Studies 105 to date have, however, focused on fruit stones such as grape and olive, while the application of geometric morphometrics to cereal grains has been treated with caution due to the known shape distortion caused by charring of starch-rich grain compared with the relative shape stability of woody fruit stones.…”

Digital seed morphometry for genotype identification – Case study of seeds of excavated (15th century Hungary) and current vinegrape (Vitis v. vinifera) varieties

“…In a study using KS-400V.3.0 program (Carl Zeiss, Germany) thirteen seed shape and size parameters of the total 93 measured characters were found to be discriminative (Orrù et al 2013). Of the thirteen parameters the perimeter ratio (P conv /P crof ), maximum diameter (D max ), feret ratio (D min /D max ), equivalent circular diameter (Ecd), and convex perimeter (Pconv) were found the most discriminative (Orrù et al 2013).…”

“…Of the thirteen parameters the perimeter ratio (P conv /P crof ), maximum diameter (D max ), feret ratio (D min /D max ), equivalent circular diameter (Ecd), and convex perimeter (Pconv) were found the most discriminative (Orrù et al 2013).…”

“…seed width divided by seed length (Stummer 1911), size ratios of seed parameters (Mangafa and Kotsakis 1996), and elliptic Fourier transformation (Milanesi et al 2011, Terral et al 2010. All these measurements provided huge data for Vitis taxonomy, and became useful for identification of excavated seed remains (Gyulai et al 2011a, b, Milanesi et al 2011, Orrù et al 2013. However, these data were taken by individual seed measurements on relatively small seed populations.…”

“…Fovea Pro 4.0 (Russ 2005(Russ , 2007, ImageJ (Chen and Manchester 2007, Rasband 1997-2006, and KS-400V.3.0 (Carl Zeiss, Germany) (Orrù et al 2013), which methods allowed to measure hundreds of seed populations at a time. The computer assisted digital seed morphometry have already provided several important and basic knowledge in plant evolution and domestication (Rovner 2011, Rovner andGyulai 2007), plant taxonomy (Brinkkemper et al 2011), and arhaeobotany (Gyulai 2012a, b, Orrù et al 2013, Rovner et al 2013).…”