Morphological effects of <i>Epiccocum nigrum</i> and its antibiotic flavipin on <i>Monilinia laxa</i>

Madrigal, C.; Melgarejo, P.

doi:10.1139/b95-043

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…Unlike most of other flavonoids, few studies have investigated the biological activities of Flavipin showing its antibacterial and antifungal properties [22, 36]. In the current study, we found that activation of Ahr by Flavipin suppressed cell proliferation and adhesion in MDA-MB-231 and T47D cells.…”

Section: Discussionsupporting

confidence: 46%

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Novel Aryl Hydrocarbon Receptor Agonist Suppresses Migration and Invasion of Breast Cancer Cells

et al. 2016

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BackgroundDespite the remarkable progress to fight against breast cancer, metastasis remains the dominant cause of treatment failure and recurrence. Therefore, control of invasiveness potential of breast cancer cells is crucial. Accumulating evidences suggest Aryl hydrocarbon receptor (Ahr), a helix-loop-helix transcription factor, as a promising target to control migration and invasion in breast cancer cells. Thus, an Ahr-based exploration was performed to identify a new Ahr agonist with inhibitory potentials on cancer cell motility.MethodsFor prediction of potential interactions between Ahr and candidate molecules, bioinformatics analysis was carried out. The interaction of the selected ligand with Ahr and its effects on migration and invasion were examined in vitro using the MDA-MB-231 and T47D cell lines. The silencing RNAs were transfected into cells by electroporation. Expressions of microRNAs (miRNAs) and coding genes were quantified by real-time PCR, and the protein levels were detected by western blot.ResultsThe in silico and in vitro results identified Flavipin as a novel Ahr agonist. It induces formation of Ahr/Ahr nuclear translocator (Arnt) heterodimer to promote the expression of cytochrome P450 family 1 subfamily A member 1 (Cyp1a1). Migration and invasion of MDA-MB-231 and T47D cells were inhibited with Flavipin treatment in an Ahr-dependent fashion. Interestingly, Flavipin suppressed the pro-metastatic factor SRY-related HMG-box4 (Sox4) by inducing miR-212/132 cluster. Moreover, Flavipin inhibited growth and adhesion of both cell lines by suppressing gene expressions of B-cell lymphoma 2 (Bcl2) and integrinα4 (ITGA4).ConclusionTaken together, the results introduce Flavipin as a novel Ahr agonist, and provide first evidences on its inhibitory effects on cancer cell motility, suggesting Flavipin as a candidate to control cell invasiveness in breast cancer patients.

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Section: Discussionsupporting

confidence: 46%

“…Interestingly, some flavonoid compounds exert their effects through activation of Ahr [20, 21]. Unlike most of the known flavonoids, few studies have been performed to study the biological significance of Flavipin [22, 23]. In addition, no studies have been performed to investigate the anticancer potential of this phenolic compound.…”

Section: Introductionmentioning

confidence: 99%

Novel Aryl Hydrocarbon Receptor Agonist Suppresses Migration and Invasion of Breast Cancer Cells

et al. 2016

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“…The observed effects were presumably the result of bioactive compounds (such as lytic enzymes or inhibitory compounds), secreted by the antagonistic isolates, that may act on C. acutatum. In fact, both C. globosum and E. nigrum have been known to produce many bioactive compounds (such as chaetoglobosins, chaetomin and chaetoviridins, and flavipin, respectively) that have revealed the capacity to inhibit colony growth or germination of several phytopathogenic fungi (Aggarwal, 2015;Biswas et al, 2012;Madrigal and Melgarejo, 1995). Both species are also known to produce lytic enzymes (e.g.…”

Section: Discussionmentioning

confidence: 99%

Fungal community in olive fruits of cultivars with different susceptibilities to anthracnose and selection of isolates to be used as biocontrol agents

et al. 2017

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A B S T R A C TOlive anthracnose is an important fruit disease in olive crop worldwide. Because of the importance of microbial phyllosphere to plant health, this work evaluated the effect of cultivar on endophytic and epiphytic fungal communities by studying their diversity in olives of two cultivars with different susceptibilities to anthracnose. The biocontrol potency of native isolates against Colletotrichum acutatum, the main causal agent of this disease, was further evaluated using the dual-culture method. Fungal community of both cultivars encompassed a complex species consortium including phytopathogens and antagonists. Host genotype was important in shaping endophytic but not epiphytic fungal communities, although some host-specific fungal genera were found within epiphytic community. Epiphytic and endophytic fungal communities also differed in size and in composition in olives of both cultivars, probably due to differences in physical and chemical nature of the two habitats. Fungal tested were able to inhibited C. acutatum growth (inhibition coefficients up to 30.9), sporulation (from 46 to 86%) and germination (from 21 to 74%), and to caused abnormalities in pathogenic hyphae. This finding could open opportunities to select specific beneficial microbiome by selecting particular cultivar and highlighted the potential use of these fungi in the biocontrol of olive anthracnose.

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“…Flavipin is produced by several Aspergillus and Epicoccum species as well as by Chaetomium globosum (Raistrick & Rudman, 1956;Bamford et al, 1961;Sekita et al, 1982). The compound is toxic to microorganisms, particularly to fungi (Raistrick & Rudman, 1956;Bamford et al, 1961;Baráthová et al, 1969;Burge et al, 1976;Brown et al, 1987;Mallea et al, 1991;Madrigal & Melgarejo, 1995), and was implicated as the mechanism responsible for the biocontrol of Monilinia laxa, a fungal plant pathogen, by Epicoccum nigrum (Madrigal et al, 1991). The effects against whole organisms other than fungi and bacteria are poorly studied except for a few reported bioassays on plants (Bamford et al, 1961;Burge et al, 1976).…”

Section: Discussionmentioning

confidence: 99%

Isolation of flavipin, a fungus compound antagonistic to plant-parasitic nematodes

Nitao¹,

Meyer²,

Oliver³

et al. 2002

Nematol

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An isolate of the fungus Chaetomium globosum produced culture broths that inhibited in vitro egg hatch and juvenile mobility of root-knot nematode (Meloidogyne incognita) and hatch of soybean cyst nematode (Heterodera glycines). Extraction and bioassay-directed fractionation of the culture broth ltrate determined that avipin, a low molecular weight compound, was the fungus metabolite responsible for most of the nematode-antagonistic activity. Synthesis of avipin permitted evaluation of the compound as a suppressor of nematode populations on plants in glasshouse studies. Muskmelon (Cucumis melo) plants in steamed and unsteamed soil were inoculated with root-knot nematodes and various concentrations of avipin were applied to the soil. Contrary to expectations from the in vitro studies, the number of galls per g of roots increased with avipin treatment at the 14-day harvest. No effect of avipin on nematode populations was found at the 55-day harvest. In general, plant growth and nematode populations were greater in plants grown in steamed soil.

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Morphological effects of Epiccocum nigrum and its antibiotic flavipin on Monilinia laxa

Cited by 14 publications

References 13 publications

Novel Aryl Hydrocarbon Receptor Agonist Suppresses Migration and Invasion of Breast Cancer Cells

Novel Aryl Hydrocarbon Receptor Agonist Suppresses Migration and Invasion of Breast Cancer Cells

Fungal community in olive fruits of cultivars with different susceptibilities to anthracnose and selection of isolates to be used as biocontrol agents

Isolation of flavipin, a fungus compound antagonistic to plant-parasitic nematodes

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