Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (<i>Hystrix javanica</i>)

Abstract

Wound healing in the Sunda porcupine is believed to occur quickly, although the wound is large and severe. Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it. The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines. Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process. Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral). Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing. The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process. LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber. In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.

Fatty acid composition profiling in the dorsal skin of Sunda porcupine (<i>Hystrix javanica</i>)

“…The TD region showed higher intensity of immunopositive reaction compared to the LS [ 28 ]. Additionally, the wound healing in dorsal skin also exhibited the site-depending factor, where the TD region healed faster than the LS [ 29 ].…”

“…The animals were used mainly for natural wound healing in line with Prawira et al . [ 29 ]. Full thickness wounding was conducted by incision method to make an open wound with an area of approximately 11.3 ± 1.23 cm 2 in each region, i.e.…”

“…They were sampled for skin biopsy and the procedures were conducted under anesthesia using 10% HCl ketamine (Ilium Ketamil, Troy Laboratories, Glendenning, NSW, Australia) and 2% xylazine HCl (Ilium Xylazil, Troy Laboratories) at doses of 2.5 mg/kg body weight (BW) and 1 mg/kg BW. The animals were used mainly for natural wound healing in line with Prawira et al [29]. Full thickness wounding was conducted by incision method to make an open wound with an area of approximately 11.3±1.23 cm 2 in each region, i.e.…”

“…Due to this special feature, this study was conducted to examine the characteristics of the Sunda porcupine skin. Previous studies have been conducted on the skin structure [ 24 ], bacterial population [ 25 ], alpha tocopherol in the skin [ 26 ], lectin binding in the sebaceous gland [ 27 ], immunoreaction of uncoupling protein 1 (UCP-1) antibody in sebaceous gland [ 28 ] and morphological evaluation of polysaccharide in wound healing [ 29 ].…”

Influência da fototerapia na cicatrização no modelo ex vivo de pele humana (hOSEC)

“…Picrosirius red, Tricrômico de Masson e Tricrômico de Gomori.Neste trabalho o uso da análise molecular através da qPCR foi escolhida para avaliar o modelo hOSEC, por ser um método quantitativo com grande sensibilidade e especificidade.Para o COL1A1 as amostras de ambas as peles estudas não apresentaram amplificação detectável. Não se pode afirmar o que acarretou a ausência de expressão gênica do colágeno do tipo I nos fragmentos em estudo, porém a hipótese está relacionada à síntese de colágeno tipo I acontecer em fases mais posteriores do processo de cicatrização, na fase de proliferação que III por fibras do tipo I. Além disso, acredita-se que o colágeno tipo III desempenhe papel no processo de fibrilogênese do colágeno tipo I. Assim, as quantidades das fibras tipo I que são produzidas em menor quantidade que as fibras do tipo III nessa fase inicial podem ter colaborado para não identificação a nível molecular(Prawira et al 2020).Em contrapartida, foi possível verificar expressão gênica do COL3A1 em todos os fragmentos e diferença estatística, somente no grupo controle entre os fragmentos da pele 2 apresentando diminuição da expressão ao longo do tempo, já nos grupos que foram tratados pelo laser não houve diferença estatística, e de forma geral mantiveram a mesma tendência que o grupo controle.Ainda ao se comparar os grupos controle e de tratamento dentro do mesmo dia de cultura, houve diferença significativa na expressão gênica de COL3A1 entre os grupos da pele 3 apenas do dia 6, sendo que a expressão gênica seguiu uma tendência de aumento entre os grupos: Controle, 660 nm, 830 nm e 830 nm + 660 nm. Alguns autores comoLeite et al (2015), Broughton e Janis (2006) e Li et al (2007) referem que os fibroblastos que se encontram na área lesionada, começam a sintetizar a matriz provisória composta de glicosaminoglicanas e colágeno do tipo III.…”