Morphological-molecular incongruence in Sphagnum majus ssp. majus and ssp. norvegicum

Nieto‐Lugilde, Marta; Robinson, Sean; Aguero, Blanka; Duffy, Aaron M.; Imwattana, Karn; Hassel, Kristian; Flatberg, Kjell Ivar; Stenøien, Hans K.; Shkurko, Anna V.; Fedosov, Vladimir E.; Shaw, A. Jonathan

doi:10.1639/0007-2745-125.2.294

Cited by 5 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Andrus and S. bartlettianum Warnst. ), it was not confirmed by molecular phylogenetic analyses (Shaw et al, 2005;Duffy et al, 2020;Nieto-Lugilde et al, 2022).…”

Section: Introductionmentioning

confidence: 89%

On the morphological delimitation Of Sphagnum henryense

Shkurko

Mamontov

Fedosov

2022

Arctoa, a jornal of bryology

View full text Add to dashboard Cite

Sphagnum henryense (subg. Sphagnum) differs from S. palustre in having abundant small pores on the dorsal surface of branch leaves. Recently studies usually consider it as a doubtful species or even merely a synonym of S. palustre. We revealed S. palustre-like plants, with numerous small round pores very similar to those of S. henryense in collection from Russian Far East, South Kuril Islands. The morphometric studies of these plants, along with S. henryense from other regions of Asia and North America and S. palustre from the same areas and Europe revealed statistically reliable differences in three traits of pores in the hyaline cells on abaxial branch leaf surface: (1) number, (2) length, (3) length to width ratio, and significant differences in (4) branch leaf breadth and (5) number of pores in stem cortex cells, distinguishing the henryense-morphotype. Such morphotypes are illustrated, discussed, and likely deserve a further studies. РезюмеSphagnum henryense (subg. Sphagnum) отличается от S. palustre наличием многочисленных мелких пор на дорсальной поверхности веточных листьев. В последнее время его часто рассматривают как сомнительный вид или даже синоним S. palustre. Недавно нами были обнаружены растения, похожие на S. palustre, с многочисленными мелкими круглыми порами, очень похожими на таковые у S. henryense в коллекции с острова Кунашир, Южные Курилы. Морфометрические исследования этих растений, наряду с S. henryense из других регионов Азии и Северной Америки, а также S. palustre из различных регионов, выявили статистически достоверные различия по трем признакам пор гиалиновых клеток на абаксиальной поверхности веточного листа: (1) количество, (2) длина, (3) отношение длины к ширине, а также значимые отличия по (4) ширине веточного листа и (5) количеству пор в клетках гиалодермиса стебля, отличающие "henryense-морфотип". Такие дальневосточные морфотипы проиллюстрированы, обсуждены и, на наш взгляд, заслуживают дальнейшего изучения.

show abstract

“…Andrus and S. bartlettianum Warnst. ), it was not confirmed by molecular phylogenetic analyses (Shaw et al, 2005;Duffy et al, 2020;Nieto-Lugilde et al, 2022).…”

Section: Introductionmentioning

confidence: 89%

On the morphological delimitation Of Sphagnum henryense

Shkurko

Mamontov

Fedosov

2022

Arctoa, a jornal of bryology

View full text Add to dashboard Cite

show abstract

“…Genomic libraries were made following the double digestion restriction site-associated DNA sequencing (ddRADseq) protocol of Parchman et al (2012) with modifications described by Duffy et al (2020) and using restriction enzymes MseI and EcoRI. The RAD-seq technique (Andrews et al, 2016) has been shown to provide sufficient data for phylogenetic and population genetic analysis of Sphagnum, including when DNA was procured from herbarium specimens (Yousefi et al, 2017(Yousefi et al, , 2019Duffy et al, 2020;Nieto-Lugilde et al, 2022;Shaw et al, 2022). Each library was cleaned and size-selected for fragments around 350 bp using AMPur XPbeads (Beckman Coulter, Jersey City, NJ, USA), checked for quality on a BioAnalyzer (Agilent Technologies, Wilmington, DE, USA), and sequenced on a single lane of Illumina HiSeq.…”

Section: Taxon Samplingmentioning

confidence: 99%

“…DNA isolation, library preparation, and sequencing DNA extractions followed the CTAB protocol outlined by Nieto-Lugilde et al (2022). Three samples of S. rubellum from the same DNA extraction (sample ID: KI093) were included in two different libraries to assess sequencing error and to allow for identification of likely clones.…”

Section: Taxon Samplingmentioning

confidence: 99%

Parallel patterns of genetic diversity and structure in circumboreal species of the Sphagnum capillifolium complex

Imwattana,

Aguero,

Nieto‐Lugilde

et al. 2024

American J of Botany

Self Cite

View full text Add to dashboard Cite

PremiseShared geographical patterns of population genetic variation among related species is a powerful means to identify the historical events that drive diversification. The Sphagnum capillifolium complex is a group of closely related peat mosses within the Sphagnum subgenus Acutifolia and contains several circumboreal species whose ranges encompass both glaciated and unglaciated regions across the northern hemisphere. In this paper, we (1) inferred the phylogeny of subg. Acutifolia and (2) investigated patterns of population structure and genetic diversity among five circumboreal species within the S. capillifolium complex.MethodsWe generated RAD sequencing data from most species of the subg. Acutifolia and samples from across the distribution ranges of circumboreal species within the S. capillifolium complex.ResultsWe resolved at least 14 phylogenetic clusters within the S. capillifolium complex. Five circumboreal species show some common patterns: One population system comprises plants in eastern North America and Europe, and another comprises plants in the Pacific Northwest or around the Beringian and Arctic regions. Alaska appears to be a hotspot for genetic admixture, genetic diversity, and sometimes endemic subclades.ConclusionsOur results support the hypothesis that populations of five circumboreal species within the S. capillifolium complex survived in multiple refugia during the last glacial maximum. Long‐distance dispersal out of refugia, population bottlenecks, and possible adaptations to conditions unique to each refugium could have contributed to current geographic patterns. These results indicate the important role of historical events in shaping the complex population structure of plants with broad distribution ranges.

show abstract

Ecological differentiation and sympatry of cryptic species in the Sphagnum magellanicum complex (Bryophyta)

Nieto‐Lugilde,

Piatkowski

et al. 2024

American J of Botany

View full text Add to dashboard Cite

PremiseSphagnum magellanicum (Sphagnaceae, Bryophyta) has been considered to be a single semi‐cosmopolitan species, but recent molecular analyses have shown that it comprises a complex of at least seven reciprocally monophyletic groups, that are difficult or impossible to distinguish morphologically.MethodsNewly developed barcode markers and RADseq analyses were used to identify species among 808 samples from 119 sites. Molecular approaches were used to assess the geographic ranges of four North American species, the frequency at which they occur sympatrically, and ecological differentiation among them. Microhabitats were classified with regard to hydrology and shade. Hierarchical modelling of species communities was used to assess climate variation among the species. Climate niches were projected back to 22,000 years BP to assess the likelihood that the North American species had sympatric ranges during the late Pleistocene.ResultsThe species exhibited parallel morphological variation, making them extremely difficult to distinguish phenotypically. Two to three species frequently co‐occurred within peatlands. They had broadly overlapping microhabitat and climate niches. Barcode‐ versus RADseq‐based identifications were in conflict for 6% of the samples and always involved S. diabolicum vs. S. magniae.ConclusionsThese species co‐occur within peatlands at scales that could permit interbreeding, yet they remain largely distinct genetically and phylogenetically. The four cryptic species exhibited distinct geographic and ecological patterns. Conflicting identifications from barcode vs. RADseq analyses for S. diabolicum versus S. magniae could reflect incomplete speciation or hybridization. They comprise a valuable study system for additional work on climate adaptation.

show abstract

Morphological-molecular incongruence in Sphagnum majus ssp. majus and ssp. norvegicum

Cited by 5 publications

References 39 publications

On the morphological delimitation Of Sphagnum henryense

On the morphological delimitation Of Sphagnum henryense

Parallel patterns of genetic diversity and structure in circumboreal species of the Sphagnum capillifolium complex

Ecological differentiation and sympatry of cryptic species in the Sphagnum magellanicum complex (Bryophyta)

Contact Info

Product

Resources

About