Morphological Peculiarities of Temperature and Osmotic Response of Erythrocytes in Presence of Chloropromazine

Shpakova, N. M.; Semionova, E.A.; Kovalenko, Igor; Iershova, N.A.; Орлова, Н. В.

doi:10.15407/fz63.05.062

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Для хлорпромазину діапазон захисних концентрацій становить 500-700 мкмоль/л, для децилсульфату натрію -200-600 мкмоль/л. Амфіпатичні речовини здатні змінювати форму клітин, об'єм та площу поверхні, можуть викликати перерозподіл фосфоліпідів між внутрішнім та зовнішнім моношаром мембрани [7,8,16,17] рез їхню двояковогнуту форму є бімодальний розподіл сигналів FSC (див. рисунок, а), який є результатом випадкової орієнтації еритроцитів у потоці [19].…”

Section: результати та їх обговоренняunclassified

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Nipot,

Yershova,

Chabanenko

et al. 2024

Fiziol Zh

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The search for protective substances that can be used during red blood cell thawing and the study of their effects on red blood cells contribute to increasing the number and quality of viable cells after the cryopreservation cycle. We studied the effect of posthypertonic shock and amphiphilic compounds on the shape and eryptosis of human erythrocytes. The method of flow cytometry was used, this allows analyzing two parameters simultaneously, which increases the efficiency of research. The shape was assessed by the sphericity index (SphI), and eryptosis by the redistribution of phosphatidylserine to the membrane outer surface. It has been shown that sodium decylsulfate and chlorpromazine reduce erythrocyte damage in posthypertonic shock by 3.6 and 4.2 times, respectively. Sodium decylsulfate helps to preserve the shape of cells (SphI coefficient remains the same), while when chlorpromazine is used, the shape changes towards spherical (SphI coefficient changes 2 times). The study of the level of Annexin V FITC binding to phosphatidylserine in outer layer of membrane revealed a concentration-dependent increase in fluorescence when sodium decylsulfate was used, indicating a disorder of the bilayer asymmetry. In contrast, chlorpromazine did not change the distribution of phosphatidylserine. Comparison of two parameters of cell viability - the sphericity coefficient and anexin binding - allowed us to choose the conditions that are optimal for the use of the studied protective substances. Namely, it is advisable to use the lowest effective concentration of sodium decylsulfate (200 mcmol/l) for protective purposes. This ensures the preservation of the cell shape and minimal impact on the membrane asymmetry.

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Section: результати та їх обговоренняunclassified

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Nipot,

Yershova,

Chabanenko

et al. 2024

Fiziol Zh

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Effect of Trifluoroperazine and Sodium Decyl Sulfate on Posthypertensive Shock of Human and Rabbit Erythrocytes

Yershova¹,

Chabanenko²,

Shpakova³

et al. 2022

Fiziol Zh

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The effects of trifluoroperazine and sodium decyl sulfate on posthypertonic shock of human and rabbit erythrocytes were studied. For this purpose, the level of hemolysis in posthypertonic shock and the percentage of potassium ions released from erythrocytes in dehydration and rehydration solutions in the presence of trifluoroperazine and sodium decyl sulfate were determined. It is shown that the protective effect of amphiphilic compounds is manifested at 0°C, but not at 37°C. There is a decrease in the level of hemolysis in a certain concentration range of each of the studied substances. It was found that human erythrocytes are more sensitive to the protective action of the studied amphiphilic compounds and are characterized by a wider range of protective concentrations. This could be explained by the different lipid composition of the erythrocyte membranes of the studied mammals. Measurement of the leak of potassium ions in dehydration and rehydration media in the presence of amphiphilic compounds suggested that the latter do not affect the permeability of the membrane of mammalian erythrocytes for potassium in posthypertonic shock. Based on the obtained results and literature data, it is assumed that the protective effects of trifluoroperazine and sodium decyl sulfate occur independenly of the formation of temporary defects in permeability for potassium ions, but involve an increase in the cell surface area due to the incorporation of amphiphilic molecules. This results to an increase in the critical hemolytic volume of erythrocytes and reduction in the level of damage during change from hypertonic conditions to isotonic ones.

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Influence of chlorpromazine on the resistance of erythrocytes of rats of different ages to hypertonic conditions

2018

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

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The osmotic stability of native and modified with chlopromazine (CPR) erythrocytes of 1- and 12-month rats to hypertonic conditions in sucrose solutions and hypertonic shock (4.0 M NaCl) has been studied. It has been shown that 2-min incubation of rat erythrocytes of different ages in hypertonic sucrose media does not reveal any differences in the osmotic stability of these cells. In this case, CPR does not affect cell hemolysis. An increase of the incubation time in hypertonic sucrose solutions to 30 minutes allowed detecting a greater osmotic sensitivity of erythrocytes of 1-month animals to the action. In this case, the protective effect of CPR for older age rat erythrocytes (12 months) has been established. It has been found that in 4.0 M NaCl the hemolysis level of animal erythrocytes of both age groups increases with preliminary incubation (2 min) in a sucrose medium with a concentration of 0.7 M and above. With increasing exposure time (30 min) in sucrose hypertonic solutions, the sensibilization of animal erythrocytes of both age groups to the action of hypertonic shock is also intensified. In this study it has been shown that the influence of CPR on the sensitivity of 1-month-old animal erythrocytes to the transfer in 4.0 M NaCl depends on the tonicity and duration of the cell initial incubation in sucrose solutions. Thus, CPR increases the osmotic resistance of erythrocytes, which were preexposed in sucrose solutions at a concentration of 0.6–0.8 M for 2 and 30 min. Some increase of hemolysis level of these animals erythrocytes modified with CPR in 4.0 M NaCl has been observed after 2 min of incubation in sucrose solutions at a concentration of 0.27–0.5 M. The exclusively protective influence of CPR on 12-month-old animal erythrocytes in conditions of hypertonic shock has been revealed. A quantitative estimation of the efficiency of CPR at hypertonic shock (4.0 M NaCl) of different age animal erythrocytes has been carried out by calculation of the antihemolytic activity value (AG). Short-term incubation (2 min) in a sucrose media does not reveal any differences in the values of AG of CPR for erythrocytes of both age groups. For the cells of young rats, increase of AG of CPR is observed at incubation in sucrose medium to 30 min and for erythrocytes of the older group – to 10 min. With an increase in incubation time of up to 60 min the CPR efficiency in hypertonic saline media is reduced for rat cells in both age groups but in varying degrees.

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Morphological Peculiarities of Temperature and Osmotic Response of Erythrocytes in Presence of Chloropromazine

Cited by 3 publications

References 12 publications

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Effect of Trifluoroperazine and Sodium Decyl Sulfate on Posthypertensive Shock of Human and Rabbit Erythrocytes

Influence of chlorpromazine on the resistance of erythrocytes of rats of different ages to hypertonic conditions

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