Morphological, physico-chemical and micropropagation studies in Jatropha curcas L. and RAPD analysis of the regenerants

Leela, T.; Naresh, B.; Reddy, Mallamma V.; Madhusudhan, N.Ch.; Cherku, Prathibha Devi

doi:10.1016/j.apenergy.2010.12.080

Cited by 24 publications

(15 citation statements)

References 29 publications

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“…This clonal propagation method has the advantage of producing plants that are morphologically homogenous with an equal production potential. Several authors have regenerated J. curcas from nodal explants, from hypocotyls, leaves, petioles and cotyledons [1]. However, the number of plantlets produced per explant remained relatively low after a period of four to six weeks (10 to 42 plantlets per explants) which is not economically viable.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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Section: Introductionmentioning

confidence: 99%

“…The seeds contain up to 38% in non-edible oil which is highly combustible [1]. The use of J. curcas for the production of biofuel could compensate for energy shortages, reduce CO 2 gas (Carbon dioxide) emissions and contribute to increase farmers' revenues [2].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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“…Although a great number of genetically identical plants can be regenerated by means of micropropagation, some somaclonal variations can be detected (Miguel and Marum, 2011;Pathak and Dhawan, 2012), which in turn depend on various factors, among which the type of explant used should be considered (Leela et al, 2011). Cultivation in vitro from explants with meristematic regions, such as apical and nodal segments, are very popular and important (Zoghlami et al, 2012), as the frequency of somaclonal variations in the regenerated plants is low (Singh et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Crambe Abyssinica Hochst. Ex R.E. Fr.

Werner¹,

Gontijo²,

Amaral³

et al. 2017

Aust J Crop Sci

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Crambe abyssinica Hochst. Ex. R. E. Fr. is an oilseed species that produces non-edible oil, initially employed in Brazil as a culture for soil surface coverage and animal feed. However, with stimulation of the production and use of renewable energies, it arose as an interesting alternative for biodiesel production. The aim of this work was to describe a protocol for micropropagation of crambe, using apical segments as explants. Crambe seeds germinated in vitro in medium MS½ provided apical segments adequate to the micropropagation process. In the shoot induction phase, testing the interaction of the cytokinins 6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ) as well as different concentrations thereof, the highest numbers of shoots per explant were obtained with 5 µM BAP at 30 days (9.26 shoots) and 15 µM BAP at 60 days (18.08 shoots) of in vitro culture. In the elongation with 1 µM gibberellic acid (GA 3 ), the mean length of the shoots turned from 3.61 cm to 5.03 cm after 30 days. The highest percentage of rooting was of 30% with 0.25 µM naphthalene-acetic acid (NAA). The results of this work revealed a satisfactory frequency of shoot regeneration from apical segments of crambe in media containing 5 µM BAP, and adequate elongation with 1 µM GA 3 . Nevertheless, there is still need for efficient rooting and acclimatization for complete in vitro propagation of crambe.

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“…Thus, it is necessary to check the crop's behavior in different applied volumes of water and identify the stages of higher consumption or critical periods where the lack or excess of water causes a growth decrease (Bernardo et al, 2009). The chronological monitoring identifies the regulatory mechanisms of periodic rhythm of growth (Abdulla et al, 2011;Leela et al, 2011). Despite using only primary data, non-destructive growth analysis is a useful and efficient tool, and may be complete if growth rates are calculated (Fontes et al, 2005;Cardoso et al, 2006;Barcelos et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Behavior and water needs of sesame under different irrigation regimes: Analysis of growth

Pereira¹,

Guerra²,

Bezerra³

et al. 2017

Afr. J. Agric. Res.

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The aim of this work was to evaluate the growth of sesame BRS 196 CNPA G4 in different irrigation depths (305, 436, 567 and 698 mm), applied on the basis of crop evapotranspiration-ETc (the depth of 567 mm was equal to 100% ETc). It was conducted at Embrapa Cotton, in Barbalha County, Ceará State, Brazil, 2012. The experiment was in randomized blocks, with treatments distributed in plots with three replications. The ETc was calculated by multiplying the reference evapotranspiration (ETo), determined by the Penman-Monteith method, by the crop coefficients (Kc), recommended for each phenological stage of the crop and by FAO. For the other irrigation treatments, the ETc was multiplied by 0.4, 0.7 and 1.3 (40, 70 and 130% of ETc). Primarily, height, stem diameter, leaf area and leaf area index were measured at 27, 48, 69 and 90 Days After Emergence (DAE), and then the absolute and relative growth rates in height, stem diameter and leaf area were estimated. It was concluded that height, stem diameter, leaf area and leaf area index of sesame BRS 196 CNPA G4 increased with irrigation; the highest growth occurred until 70 or 75 DAE, and the greatest growth was obtained, in general, in 698 mm of irrigation depth.

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Morphological, physico-chemical and micropropagation studies in Jatropha curcas L. and RAPD analysis of the regenerants

Cited by 24 publications

References 29 publications

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Micropropagation of Crambe Abyssinica Hochst. Ex R.E. Fr.

Behavior and water needs of sesame under different irrigation regimes: Analysis of growth

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