Morphological Studies of the Armillaria Mellea Complex: Two New Species, A. Gemina and A. Calvescens

Molecular Phylogeny and Evolutionary Divergence of North American Biological Species ofArmillaria

“…The combined analysis of these data sets emphasizes the value of Kluge's (1989) total evidence concept to maximize the explanatory power of the data, as well as Quicke's (1993) argument that a combination of more than one data set would increase the amount of data available for phylogenetic resolution. In this study results obtained from the combined data set were in agreement with a previous phylogenetic hypothesis (Anderson and Stasovski, 1992) and morphological groups (Bérubé and Dessureault, 1989).…”

“…This in turn affected the placement of NABS II in the molecular clock, making it appear ancestral to NABS I. However, NABS I and II have been shown to be very similar based on morphological characters (Bérubé and Dessureault, 1989) and molecular characters Anderson and Stasovski, 1992;Miller et al, 1994), with the exception of host preference and base substitutions from anonymous sequences in this study (Figs. 3A and 3D).…”

“…2). The high degree of similarity in DNA (Miller et al, 1994) and fruit body morphology (Bérubé and Dessureault, 1989) between NABS I and II, and the presence of intersterility barriers producing separate biological species (Bérubé and Dessureault, 1989), indicates that divergence has occurred. However, the direction of this divergence is questionable.…”

“…NABS III and VII were both found in the same rDNA class . They show similarity in cluster analyses of RFLPs of mtDNA (Smith and Anderson, 1989) and in fruit body morphology (Bérubé and Dessureault, 1989) and are considered closely related species based on IGR sequences of rDNA (Anderson and Stasovski, 1992). They are also found as weak pathogens or saprophytes on hardwood hosts.…”

Identification protocol for sixArmillariaspecies from northeastern North America

“…A variety of identification techniques have been developed and used since the 1970s. Nonmolecular species identification techniques include those based on basidiocarp morphology (Bérubé and Dessureault 1989;Fox et al 1994), in vitro isolate pairing (Korhonen 1978;Guillaumin et al 1991;Rizzo and Harrington 1992), and various protein-based analyses (Pérez-Sierra et al 2000). These species identification techniques are being supplanted by the emergence and rapid development of molecular methods.…”