Morphological study of recombinant human transforming growth factor β1-induced intramembranous ossification in neonatal rat parietal bone

Takano, Teruo; Taniguchi, Yasuyuki; Gotoh, Kohsei; Satoh, Ryohei; Inazu, Mizuho; Ozawa, Hiroki

doi:10.1016/8756-3282(93)90237-5

Cited by 55 publications

(32 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD45 + and CD31 + expression are displayed as the same data set, because those antibodies were expressed by periosteal cells in almost identical numbers and temporal profiles. intramembranous ossification by differentiating into osteoblasts [40]. Periosteum serves as a reservoir of osteochondral precursor cells [46] and represents a new candidate for cell-based therapies.…”

Section: Discussionmentioning

confidence: 99%

“…The cambium layer is highly cellular and contains numerous cell types, including fibroblasts, osteoblasts, and osteochondral precursor cells [47]. Mesenchymal precursor cells in the periosteum differentiate into neochondrocytes, producing cartilage tissue during embryogenesis and contributing to bone apposition during intramembranous ossification by differentiating into osteoblasts [40].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering. Questions/purpose We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro. Methods We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1 À and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription-polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization. Results Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…Another possibility is that growth factors accumulated in the bone matrix, such as bone morphogenetic proteins (Iwasaki et al, 1994;Suzawa et al, 1999;Yamaguchi et al, 1991), transforming growth factors-␤ (Mackie et al, 1998;Tanaka et al, 1993), insulin like growth factors (Chihara and Sugimoto, 1997) and basic fibroblast growth factors (Iseki et al, 1997) may stimulate the cells on the bone surface to differentiate to osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of periosteum‐derived cells is promoted by the physical contact with the bone matrix in vivo

et al. 2001

View full text Add to dashboard Cite

The periosteum contains osteoprogenitors that differentiate to osteoblasts in bone growth or repair. Our previous studies suggested the hypothesis that the physical contact of the periosteum with the bone matrix is requisite for the differentiation of osteoblasts. To test the hypothesis, the present study was designed to investigate how the contact between the periosteum and the bone matrix influences the osteoblastic differentiation of periosteal cells with establishing a new experimental model in vivo. Differentiation of osteoblasts was assessed by gene expression of type I collagen, osteocalcin and bone sialoprotein using in situ hybridization. A barrier was designed to prevent periosteal cells from contacting the bone matrix using the membrane filter. The membrane filter was inserted surgically between the surface of rat parietal bone and the periosteum after being punched out with pin holes. Periosteal cells were allowed to contact with the bone surface only through the pin holes. The pin hole was filled with cells derived from the periosteum 1 week after inserting the filter. Differentiation of osteoblasts in week 2 and noticeable bone formation in week 3 were identified on the bone surface only under the pin hole but not under the filter. The present study demonstrated that the physical contact with the bone matrix promotes osteoblastic differentiation of periosteumderived cells in vivo. Anat Rec 264:72-81, 2001.

show abstract

“…TGF-βs influence a variety of tissues, generally stimulating cells of mesenchymal origin and inhibiting cells of ectodermal origin [39]. TGF-β is produced by osteoblasts and is stored in the bone matrix, making bone the largest reservoir of TGF-β in the body [ Whereas BMPs induce bone in heterotopic sites, stimulation of bone formation by TGF-β depends on orthotopic application, for example, subperiosteal injection [65]. TGF-β1 enhances the healing of experimentally created defects of the skull in rabbits [11,47] and bone ingrowth in porous titanium rods [64] and tricalcium phosphate coated implants in dogs [40].…”

Section: Tgf-βmentioning

confidence: 99%

Growth factors in bone

Solheim¹

1998

International Orthopaedics

171

View full text Add to dashboard Cite

Morphological study of recombinant human transforming growth factor β1-induced intramembranous ossification in neonatal rat parietal bone

Cited by 55 publications

References 21 publications

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Osteoblastic differentiation of periosteum‐derived cells is promoted by the physical contact with the bone matrix in vivo

Growth factors in bone

Contact Info

Product

Resources

About