The aim of the paper is to identify and assess the factors for the development of ecotourism in the Fruška Gora National Park and to predict possible benefits for strengthening the rural economy of the area. Literature and field research was done to gather information related to geographical location, biodiversity of habitats and species, elements of cultural heritage and infrastructure of the area, as well as demographic and socio-economic status of the local community. To evaluate constraints and future possibilities of ecotourism in the area, SWOT and PESTEL analyses were used. It was found that the convenient location, substantial wildlife resources, abundance in water and geological elements, and the richness of the cultural-historical monuments are an important touristic potential of the area. The main conclusion of the work is that the development of sustainable ecotourism should create the necessary conditions for the rural development, if strategic and financial support is provided.