Morphologically Intact Melanoma Cells May Be Detected in Peripheral Blood of Melanoma Patients

Benez, Anja; Schiebel, Ursel; Fierlbeck, Gerhard

doi:10.1007/978-3-642-59537-0_11

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…The assay allows the detection, enumeration, and further characterization of the isolated cells. We demonstrated that the sensitivity of the assay is in the range of the data published for RT-PCR and that MCSP-positive melanoma cells can be detected in the peripheral blood of patients with advanced melanoma (4,5). In a novel, prospective study of chemonaive melanoma patients we have now applied the method with minor modifications to blood samples from 164 melanoma patients to determine a possible correlation with clinical stage, with the presence of manifest disease, and with the survival of the patients.…”

Section: Introductionmentioning

confidence: 74%

Immunomagnetic Enrichment, Genomic Characterization, and Prognostic Impact of Circulating Melanoma Cells

Ulmer

Schmidt‐Kittler

Fischer

et al. 2004

Clinical Cancer Research

111

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Purpose: The finding of melanoma cells in the peripheral blood, thus far mainly inferred from the PCR-based demonstration of tyrosinase mRNA, has been associated with metastatic melanoma. Neither the malignant nature nor the prognostic significance of circulating cells could be established. To address this question, we analyzed immunomagnetically isolated circulating melanoma cells for chromosomal aberrations and performed a clinical follow-up study of the enrolled patients.Experimental Design: In a prospective study, blood samples were taken from 164 melanoma patients and 50 donors without malignant disease. Circulating melanoma cells were enriched by immunomagnetic cell sorting using a murine monoclonal antibody against the melanoma-associated chondroitin sulfate proteoglycan. To prove the malignant origin of the positive cells and to define their chromosomal aberrations, we analyzed the genomes of 15 individually isolated cells from seven patients by single-cell comparative genomic hybridization (SCOMP).Results: Absolute and relative frequencies of circulating melanoma cells were associated with stage and with the presence or absence of detectable tumor. The detection of two or more cells correlated significantly with a reduced survival of patients with metastatic melanoma. All of the cells that were analyzed by SCOMP displayed multiple chromosomal changes and carried aberrations typical for melanoma.Conclusions: Immunomagnetic enrichment enables isolation and genomic characterization of circulating melanoma cells. The prognostic impact on survival of metastatic patients apparently reflects the aggressiveness of an ongoing tumor spread. Direct genomic analysis of the enriched and isolated cells will help to clarify the molecular-genetic basis of the establishment of generalized melanoma.

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Section: Introductionmentioning

confidence: 74%

Immunomagnetic Enrichment, Genomic Characterization, and Prognostic Impact of Circulating Melanoma Cells

Ulmer

Schmidt‐Kittler

Fischer

et al. 2004

Clinical Cancer Research

111

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“…The use of PCR and PCR-based analysis offered a high level of efficiency: Cytometry can reliably detect one (1) melanoma cell per millilitre of whole blood 22. Using PCR-based methods the reliable detection potential is one malignant cell out of 10 6 –10 7 , which corresponds approximately to one malignant cell in 1–10 mL of blood), up to 10 times better than cytometry and better than that of other techniques such as light microscopy (one malignant cell out of 10 2 –10 3 ) and immunocytochemistry (one malignant cell out of 10 4 –10 5 ) at least when cell-enrichment methods are not used 23…”

Section: Discussionmentioning

confidence: 99%

Detection of tumour cells in the bloodstream of patients with uveal melanoma: influence of surgical manipulation on the dissemination of tumour cells in the bloodstream

et al. 2015

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The change in the percentage of patients with detected tumour cells in their bloodstream was not statistically significant. The frequent shifts from negative to positive samples as well as from positive to negative samples comparing preoperative to postoperative samples indicates discontinuous shedding or variation due to measurements close to the threshold of detection. As a conclusion, the surgical manipulation does not seem to have a measurable contribution to the spread of melanoma cells in the bloodstream.

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“…One melanoma cell per ml of whole blood could be detected reliably with this assay (Benez et al 2001). Immunostaining for mAb 9.2.27 is found in about 95% of primary uveal melanomas examined (Li et al 2003).…”

Section: Immunomagnetic Melanoma Cell Enrichmentmentioning

confidence: 97%

Circulating melanoma cells in peripheral blood of patients with uveal melanoma before and after different therapies and association with prognostic parameters: a pilot study

Suesskind¹,

Ulmer

Schiebel

et al. 2009

Acta Ophthalmologica

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ABSTRACT.Purpose: To evaluate whether tumour therapy for malignant uveal melanoma leads to a shedding of melanoma cells into the systemic circulation. Methods: Ninety-four peripheral blood samples from 81 patients with malignant uveal melanoma were collected before and after different tumour therapies and the number of circulating melanoma cells (CMCs) was investigated (seven patients with enucleation, 49 patients with stereotactic radiotherapy, 19 patients with endoresection of the tumour, 15 patients with ruthenium-brachytherapy and four patients with transpupillary thermotherapy). A cellular approach was used to detect CMCs through an immunocytological assay with tumour cell enrichment by immunomagnetic cell sorting. The number of CMCs was analysed further according to specific patient characteristics, tumour parameters and the development of metastasis. Results: There was no significant difference between the number of CMCs before and after the different therapies (p = 0.78). There was also no significant association between established prognostic parameters of primary uveal melanoma and the detection of CMCs (all p >0.05). The number of CMCs was not related to the development of metastasis in a short median follow-up time of 16 months (p > 0.05). Conclusion: No changes in CMC values were observed before and after different tumour therapies. In the majority of cases therapy does not lead to a shedding of detectable melanoma cells into the systemic circulation.

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Morphologically Intact Melanoma Cells May Be Detected in Peripheral Blood of Melanoma Patients

Cited by 9 publications

References 15 publications

Immunomagnetic Enrichment, Genomic Characterization, and Prognostic Impact of Circulating Melanoma Cells

Immunomagnetic Enrichment, Genomic Characterization, and Prognostic Impact of Circulating Melanoma Cells

Detection of tumour cells in the bloodstream of patients with uveal melanoma: influence of surgical manipulation on the dissemination of tumour cells in the bloodstream

Circulating melanoma cells in peripheral blood of patients with uveal melanoma before and after different therapies and association with prognostic parameters: a pilot study

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