1988
DOI: 10.1002/jmv.1890240205
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Morphology and development of Rift Valley fever virus in vero cell cultures

Abstract: Rift Valley fever virus (RVFV) grown in vero cell cultures has a completed replication cycle within 13 hours. The first signs are the appearance of intranuclear fibrillar rods, followed by aggregations of precursor viral material in host cell cytoplasm and viral nucleocapsids budding into vacuoles associated with the Golgi apparati. Mature particles, liberated by the disintegration of vero cells, contained ribosomelike structures within the nucleocapsid, which was surrounded by a typical unit membrane through … Show more

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Cited by 34 publications
(22 citation statements)
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“…The individual capsomeres were generally uniform in appearance and apparently organized on the virus surface in a tightly packed regular lattice. The sizes and morphologies of the RVFV MP-12 particles and their capsomeres were similar to those in earlier descriptions of negatively stained wild-type RVFV (13,23,26).…”
Section: Concentration and Purification Of Rvfv Mp-12supporting
confidence: 81%
See 1 more Smart Citation
“…The individual capsomeres were generally uniform in appearance and apparently organized on the virus surface in a tightly packed regular lattice. The sizes and morphologies of the RVFV MP-12 particles and their capsomeres were similar to those in earlier descriptions of negatively stained wild-type RVFV (13,23,26).…”
Section: Concentration and Purification Of Rvfv Mp-12supporting
confidence: 81%
“…The lipid bilayer contains the viral glycoproteins G N (also termed G1) and G C (also termed G2), which project as spikes from the viral surface. Earlier studies of negatively stained RVFV showed the RNP complexes surrounded by a membrane through which surface spikes measuring 10 to 18 nm in length and ϳ5 nm in diameter were inserted (13). The virions appeared to have a distinct surface structure composed of small round capsomeres with a central cavity.…”
mentioning
confidence: 96%
“…20,50 The two potential glycosylation sites that are present in the G N and NSm sequences have been shown to be modified in P78, an indication that both the NSm and G N ectodomains are oriented towards the lumen of the endoplasmic reticulum. 51 Such a topology favors a direct interaction between P78 and the G C molecules processed from the same precursor and is consistent with the rapid detection of a P78/G C heterodimer in pulse-chase experiments.…”
Section: Discussionmentioning
confidence: 99%
“…More importantly, these approaches do not address the threat that RVFV poses as an agent for bioterrorism. The virus is widespread in diverse parts of Africa and can be propagated easily and efficiently in vitro (14). Because of its disease potential, aerosolized RVFV could be used as a weapon to threaten human life and to devastate livestock and the economy (11,39).…”
mentioning
confidence: 99%