Morphology and molecular characterization of the epiphytic dinoflagellate Amphidinium massartii, isolated from the temperate waters off Jeju Island, Korea

Lee, Kyung Ha; Jeong, Hae Jin; Park, Kila; Kang, Nam Seon; Yoo, Yeong Du; Lee, Moo Joon; Lee, Jin Woo; Lee, Soojin; Kim, Tae‐Kyung; Kim, Hyung Seop; Noh, Jae Hoon

doi:10.4490/algae.2013.28.3.213

Cited by 13 publications

(18 citation statements)

References 48 publications

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“…Jeong et al (2012b) therefore suggested that the Korean strain of C. canariensis was closely related to the two Atlantic strains and the as its overall shape and body scales, coincides with that of an A. massartii strain isolated from Australia (Murray et al 2012. Moreover, the sequences of 5.8S, ITS2, and cytochrome b of Korean A. massartii strains (AMJJ1, AMJJ2, and AMJJ3) were identical with those of the Australian A. massartii CS-259 (Lee et al 2013). Recent phylogenetic and toxicity analyses conducted by Karafas et al (2017) suggested that the strains in the nontoxic A. massartii clade 1 and the toxic A. cf.…”

Section: Coolia Sppmentioning

confidence: 62%

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Benthic dinoflagellates in Korean waters

Lim¹,

Jeong²

2021

ALGAE

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The occurrence of benthic dinoflagellates, many of which are known to be toxic, is a critical concern for scientists, government officers, and people in the aquaculture, dining, and tourism industries. The interest in these dinoflagellates in countries with temperate climate is increasing because tropical or subtropical species introduced into temperate waters by currents are able to survive the winter season in the new environment owing to global warming. Recently, several species from the benthic dinoflagellate genera Amphidinium, Coolia, Ostreopsis, Gambierdiscus, and Prorocentrum have been reported in the waters of the South and East Sea of Korea. The advent of the benthic dinoflagellates in Korean waters is especially important because raw or slightly cooked seaweeds, which may harbor these benthic dinoflagellates, as well as raw fish, which can be potentially intoxicated by phytotoxins produced by some of these benthic dinoflagellates, are part of the daily Korean diet. The recent increase in temperature of Korean coastal waters has allowed for the expansion of benthic dinoflagellate species into these regions. In the present study, we reviewed the species, distribution, and toxicity of the benthic dinoflagellates that have been reported in Korean waters. We also provided an insight into the ecological and socio-economic importance of the occurrence of benthic dinoflagellates in Korean waters.

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Section: Coolia Sppmentioning

confidence: 62%

“…in the coastal waters of Jeju Island. Later, detailed morphological and molecular characteristics of Amphidinium massartii were revealed through SEM and phylogenetic analyses (Lee et al 2013). The morphology of the Korean strain A. massartii AMJJ1, such East Sea of Korea.…”

Section: Prorocentrum Sppmentioning

confidence: 99%

Benthic dinoflagellates in Korean waters

Lim¹,

Jeong²

2021

ALGAE

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“…Fixed samples were washed three times in 1.5 mL of 5 • C ultrapure water and centrifuged at 3000× g (4 • C) for 5 min. The cells then underwent a graded ethanol (EtOH) dehydration series (10,20,30,40,50,60, 70, 80, 90, and 99%; 1.5 mL each). At every step, the cells were gently resuspended for 1 min and centrifuged under the previous conditions, and the supernatant was discarded.…”

Section: Cell Morphology and Statistical Analysismentioning

confidence: 99%

Morphological and Molecular Characterization of the Benthic Dinoflagellate Amphidinium from Coastal Waters of Mexico

Durán-Riveroll,

Juárez,

Okolodkov

et al. 2023

Phycology

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The genus Amphidinium Clap. & J. Lachm. comprises a high diversity of planktonic and benthic (epiphytic and sand-dwelling) dinoflagellates from marine and freshwater ecosystems. High morphological plasticity and vaguely defined genus characteristics (e.g., a small epicone size) have complicated the clear delineation of species boundaries. Although six Amphidinium morphospecies have been reported from Mexican coastal waters, species identifications are uncertain and not generally supported by molecular phylogenetic data. In this study, seven isolates of Amphidinium from diverse benthic coastal locations on the NE Pacific, Gulf of California, and southern Gulf of Mexico were subjected to critical morphological analysis using photonic and scanning electron microscopy. The phylogenetic reconstruction was based on nuclear-encoded, partial large-subunit (LSU) rDNA and internal transcribed spacer I and II (ITS1 and ITS2) sequences. The revised phylogenetic analysis was consistent with the traditional subdivision of the genus Amphidinium into two sister groups: Herdmanii and Operculatum clades. This study provided the first confirmed records of A. theodorei and A. massartii from coastal waters of Mexico. The molecular phylogenetic evidence indicated that the morphologically described A. cf. carterae from Baja California was in fact more closely allied with A. eilatiensis sequences. A few Amphidinium species are known to form toxigenic (i.e., fish-killing) harmful algal blooms worldwide, and therefore knowledge on species diversity and biogeography is critical in developing effective strategies for evaluating the potential emerging threat in Mexican coastal waters.

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“…The rDNA sequences of the two species were identical to those of the Korean strain (Ostreopsis cf. ovata [52]; Amphidinium massartii [53]).…”

Section: Dna Extraction and Species Identificationmentioning

confidence: 99%

Detection of the Benthic Dinoflagellates, Ostreopsis cf. ovata and Amphidinium massartii (Dinophyceae), Using Loop-Mediated Isothermal Amplification

Lee¹,

Hwang²,

Hyung³

et al. 2021

JMSE

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For the in situ and sensitive detection of benthic dinoflagellates, we have established an integrated loop-mediated isothermal amplification (LAMP) assay based on Ostreopsis cf. ovata and Amphidinium massartii. To detect the two species, a set of species-specific primers was constructed between the ITS gene and D1–D6 LSU gene, and the reaction temperature, time, and buffer composition were optimized to establish this method. In addition, the specificity of the LAMP primers was verified both in strains established in the laboratory and in field samples collected from the Jeju coastal waters, Korea. With the LAMP assay, the analysing time was within 45 to 60 min, which may be shorter than that with the conventional PCR. The detection sensitivity of the LAMP assay for O. cf. ovata or A. massartii was comparable to other molecular assays (PCR and quantitative PCR (qPCR)) and microscopy examination. The detection limit of LAMP was 0.1 cell of O. cf. ovata and 1 cell of A. massartii. The optimized LAMP assay was successfully applied to detect O. cf. ovata and A. massartii in field samples. Thus, this study provides an effective method for detecting target benthic dinoflagellate species, and could be further implemented to monitor phytoplankton in field surveys as an altenative.

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Morphology and molecular characterization of the epiphytic dinoflagellate Amphidinium massartii, isolated from the temperate waters off Jeju Island, Korea

Cited by 13 publications

References 48 publications

Benthic dinoflagellates in Korean waters

Benthic dinoflagellates in Korean waters

Morphological and Molecular Characterization of the Benthic Dinoflagellate Amphidinium from Coastal Waters of Mexico

Detection of the Benthic Dinoflagellates, Ostreopsis cf. ovata and Amphidinium massartii (Dinophyceae), Using Loop-Mediated Isothermal Amplification

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