Morphology of bakers' yeast and dissolved oxygen saturation during fed-batch growth

Gélinas, Pierre; Goulet, J.

doi:10.1111/j.1472-765x.1991.tb00531.x

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…In aerobic fermentation processes oxygen is the key substrate. Numerous effects of the extent of oxygen supply on microbial systems are reported [10,13,20,25,26,32,34,35]. The maximum oxygen transfer capacity (OTR max ) in shaken bioreactors depends on the surface-to-volume ratio of the rotating liquid including the wetted reactor wall [27].…”

Section: Introductionmentioning

confidence: 99%

Application of an improved continuous parallel shaken bioreactor system for three microbial model systems

Akgün

Müller

Engmann

et al. 2008

Bioprocess Biosyst Eng

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A continuous parallel shaken bioreactor system, combining the advantages of shaken bioreactors with the advantages of continuous fermentation, was specifically manufactured from quartz glass and provides a geometric accuracy of <1 mm. Two different model systems (facultative anaerobic bacterium C. glutamicum, and Crabtree-negative yeast P. stipitis), whose growth behaviour and metabolite formation are affected by dilution rate and oxygen availability, were studied. The transition from non-oxygen to limited conditions as function of the dilution rate could precisely be predicted applying the approach described by Maier et al. (Biochem Eng J 17:155-167, 2004). In addition, the Crabtree-positive yeast S. cerevisiae was simultaneously studied in the continuous parallel shaken bioreactor system and in a conventional 1-L bioreactor, for comparison. Essentially the same results were obtained in both types of bioreactors. However, many more reading points were obtained with the parallel shaken bioreactor system in the same time at much lower consumption of culture media.

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Section: Introductionmentioning

confidence: 99%

Application of an improved continuous parallel shaken bioreactor system for three microbial model systems

Akgün

Müller

Engmann

et al. 2008

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

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Effects of growth conditions on mitochondrial morphology inSaccharomyces cerevisiae

Visser

Spronsen²,

Nanninga³

et al. 1995

Antonie van Leeuwenhoek

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Effects of growth conditions on mitochondrial morphology were studied in living Saccharomyces cerevisiae cells by vital staining with the fluorescent dye dimethyl-aminostyryl-methylpyridinium iodine (DASPMI), fluorescence microscopy, and confocal-scanning laser microscopy. Cells from respiratory, ethanol-grown batch cultures contained a large number of small mitochondria. Conversely, cells from glucose-grown batch cultures, in which metabolism was respiro-fermentative, contained small numbers of large, branched mitochondria. These changes did not significantly affect the fraction of the cellular volume occupied by the mitochondria. Similar differences in mitochondrial morphology were observed in glucose-limited chemostat cultures. In aerobic chemostat cultures, glucose metabolism was strictly respiratory and cells contained a large number of small mitochondria. Anaerobic, fermentative chemostat cultivation resulted in the large, branched mitochondrial structures also seen in glucose-grown batch cultures. Upon aeration of a previously anaerobic chemostat culture, the maximum respiratory capacity increased from 10 to 70 mumole.min-1.g dry weight-1 within 10 h. This transition resulted in drastic changes of mitochondrial number, morphology and, consequently, mitochondrial surface area. These changes continued for several hours after the respiratory capacity had reached its maximum. Cyanide-insensitive oxygen consumption contributed ca. 50% of the total respiratory capacity in anaerobic cultures, but was virtually absent in aerobic cultures. The response of aerobic cultures to oxygen deprivation was qualitatively the reverse of the response of anaerobic cultures to aeration. The results indicate that mitochondrial morphology in S. cerevisiae is closely linked to the metabolic activity of this yeast: conditions that result in repression of respiratory enzymes generally lead to the mitochondrial morphology observed in anaerobically grown, fermenting cells.

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Morphology of bakers' yeast and dissolved oxygen saturation during fed-batch growth

Cited by 2 publications

References 17 publications

Application of an improved continuous parallel shaken bioreactor system for three microbial model systems

Application of an improved continuous parallel shaken bioreactor system for three microbial model systems

Effects of growth conditions on mitochondrial morphology inSaccharomyces cerevisiae

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