Morphology of the bone marrow, spleen and liver during hematopoietic cell mobilization with cyclophosphamide in mice.

Barcew, K; Karbicka, Anna; Szumilas, Paweł; Marchlewicz, Mariola; Grzegrzółka, Ryszard; Wiszniewska, Barbara

doi:10.2478/v10042-008-0063-y

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Of note, we have found that CTX-induced DC expansion was preceded by proliferation of cells with DC phenotype (CD11c + CD11b + Ly6G − ) in the BM early (2–3 days) after CTX treatment [43]. This observation is consistent with the capability of CTX to induce mobilization of hematopoietic stem cells from BM to circulation alone or in combination with granulocyte-colony stimulating factor (G-CSF) [42; 44; 45; 46; 47; 48; 49]. These hematopoietic stem cells harvested from peripheral blood of cancer patients treated with CTX gave rise to higher yield of DCs in vitro than their control counterpart [50; 51; 52; 53; 54].…”

Section: Introductionmentioning

confidence: 64%

Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo

Salem

El‐Naggar

Cole

2010

Cellular Immunology

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We have shown recently that cyclophosphamide (CTX) treatment induced a marked increase in the numbers of immature dendritic cells (DCs) in blood, coinciding with enhanced antigen-specific responses of the adoptively transferred CD8 + T cells. Because this DC expansion was preceded by DC proliferation in bone marrow (BM), we tested whether BM post CTX treatment can generate higher numbers of functional DCs. BM was harvested three days after treatment of C57BL/6 mice with PBS or CTX and cultured with GM-CSF/IL-4 in vitro. Compared with control, BM from CTXtreated mice showed faster generation and yielded higher numbers of DCs with superior activation in response to toll-like receptor (TLR) agonists. Vaccination with peptide-pulsed DCs generated from BM from CTX-treated mice induced comparable adjuvant effects to those induced by control DCs. Taken together, post CTX BM harbors higher numbers of DC precursors capable of differentiating into functional DCs, which be targeted to create host microenvironment riches in activated DCs upon treatment with TLR agonists.

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Section: Introductionmentioning

confidence: 64%

Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo

Salem

El‐Naggar

Cole

2010

Cellular Immunology

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“…This outcome supports findings by Barcew et al who demonstrated morphological destructive changes in the bone marrow and spleen of CP-treated mice. 64 Specifically, they observed significant decreases in cells on day 2 after intraperitoneal injection of 200 mg/g bw. A significant impact of CP on bone marrow, the primary site for hematopoiesis, could explain the time-related decreases in granulocyte levels in the other two sites as a result of the administration of 200 mg/g bw of CP.…”

Section: Discussionmentioning

confidence: 96%

Effect of Cyclophosphamide Treatment on Central and Effector Memory T Cells in Mice

et al. 2018

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Immunological memory is a key feature of adaptive immunity. It provides the organism with long-lived and robust protection against infection. The important question is whether cyclophosphamide (CP), as immunosuppressive agent used in cancer therapy and in some autoimmune diseases, may act on the memory T-cell population. We investigated the effect of CP on the percentage of central memory T cells (T) and effector memory T cells (T) in the mouse model of CP-induced immunosuppression (8-10-week-old male C57BL/6 mice CP treated for 7 days at the daily dose of 50 μg/g body weight [bw], manifested the best immunosuppression status, as compared to lower doses of CP: 10 or 20 μg/g bw). The CP induced a significant decrease in the percentage of CD8 (T), compared to nonimmunosuppressed mice. This effect was not observed in the case of CD4 T population. The percentage of gated T with CD4 and CD8 phenotype was significantly decreased in CP-treated mice, as compared to the control ones. Taken together, the above data indicate that CP-induced immunosuppression in mice leads to a reduction in the abundance of central memory cells possessing preferentially CD8 phenotype as well as to a reduction in the percentage of effector memory cells (splenocytes both CD4 and CD8), compared to the cells from nonimmunosuppressed mice. These findings in mice described in this article may contribute to the understanding of the complexity of the immunological responses in humans and extend research on the impact of the CP model of immunosuppression in mice and memory T-cell populations.

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Immunosuppressive effect of cyclophosphamide in Nile tilapia (Oreochromis niloticus)

Elazab

Younes

Gaafar

et al. 2021

Environ Sci Pollut Res

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Morphology of the bone marrow, spleen and liver during hematopoietic cell mobilization with cyclophosphamide in mice.

Cited by 3 publications

References 32 publications

Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo

Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo

Effect of Cyclophosphamide Treatment on Central and Effector Memory T Cells in Mice

Immunosuppressive effect of cyclophosphamide in Nile tilapia (Oreochromis niloticus)

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