Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Abstract

-Twelve healthy primiparous sows received intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period preceding parturition. Mammary gland biopsy samples were taken immediately before inoculation (0 h) and from the inoculated and the contralateral noninoculated glands 24 h after inoculation. The analyses of interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) by immunohistochemistry revealed that the production of these proinflammatory cytokines significantly increased in the inoculated mammary glands of sows that developed clinical signs of mastitis (affected group, n = 4) 24 h after inoculation. This was also true for IL-8 in the inoculated mammary glands of sows that did not develop clinical signs of mastitis (nonaffected group, n = 8). Sows that developed clinical signs of mastitis displayed significantly lower constitutive production of IL-1β than did sows that remained clinically healthy. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.cytokine / immunohistochemistry / mastitis / pig / E. coli

Lactobacillus johnsonii L531 Ameliorates Escherichia coli-Induced Cell Damage via Inhibiting NLRP3 Inflammasome Activity and Promoting ATG5/ATG16L1-Mediated Autophagy in Porcine Mammary Epithelial Cells

“…We found that pre-incubation with L. johnsonii L531 restricts E. coli -induced NLRP3 inflammasome activation, including the expression of receptor protein NLRP3, the adaptor protein ASC, the effector caspase-1 p10 and the release of Il-1β and Il-18 . Consistent with other studies in vivo and the microarray analysis results [ 48 , 49 , 50 , 51 ], the expression of cytokines and chemokines, such as Il-6 , Il-8 , Tnf-α , and Cxcl2 was up-regulated after E. coli challenge, whereas this increase was attenuated by pre-incubation with L. johnsonii L531. L. johnsonii L531 inhibits the activation of NLRP3 inflammasome during E. coli infection.…”

Gene expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli and Staphylococcus aureus in vitro

“…Inflammation response of periparturient sows after inoculation of porcine mammary gland with different potential mastitis-causing E. coli strains specified a dominant role of that pathogen species in CM [ 10 ]. The development of clinical symptoms of CM in the sow was suggested to be associated with a locally increased production of pro-inflammatory cytokines such as interleukin 1-beta (IL1-beta), IL6, IL8, and tumor necrosis factor-alpha (TNF-alpha) in response to intramammary E. coli infection [ 10 , 11 ]. Additionally, the time of infection of the mammary gland relative to parturition and the number of circulating neutrophils at the time of infection were shown to influence the development of clinical CM in the sow [ 12 ].…”

Effect of dietary addition of seaweed and licorice on the immune performance of pigs

“…Therefore, the licorice‐treated groups did not respond to the increase in TNF‐α mRNA, which was possibly induced by the SRBC sensitization. IL‐1β, IL‐6 and TNF‐α are known to be inflammation‐related cytokines (Zhu et al . 2007).…”

“…Therefore, the licorice-treated groups did not respond to the increase in TNF-a mRNA, which was possibly induced by the SRBC sensitization. IL-1b, IL-6 and TNF-a are known to be inflammation-related cytokines (Zhu et al 2007). The expression of IL-1b and IL-6 in the licorice-treated group showed relatively lower levels compared with those in the control group, although these differences were not statistically significant.…”