Morphometric study of nucleolar organiser regions (AgNOR) in HPV-associated precancerous lesions and microinvasive carcinoma of the oral cavity

Muzio, Lorenzo Lo; Mignogna, Michele Davide; Staibano, Stefania; Vico, Gionata De; Salvatore, G; Damiano, Simona; Bucci, E; Procaccini, Maurizio; Mezza, E.; Rosa, Gaetano De

doi:10.1016/s0964-1955(97)00003-1

Cited by 20 publications

(22 citation statements)

References 60 publications

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“…In comparison to the literature, we have observed the highest infection percentages in every pathologic stage, although comparable infection percentages have been reported by others (10,18,21,33,37,45). This finding suggests that the virus may be involved in early stages of oral carcinogenesis.…”

Section: Discussionsupporting

confidence: 89%

“High Risk” HPV Types Are Frequently Detected in Potentially Malignant and Malignant Oral Lesions, But Not in Normal Oral Mucosa

et al. 2000

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Studies on the involvement of the human papillomavirus (HPV) in initiation and progression of oral neoplasia have generated conflicting results. The observed discrepancy is attributable mainly to the varying sensitivity of the applied methodologies and to epidemiologic factors of the examined patient groups. To evaluate the role of HPV in oral carcinogenesis, we analyzed 53 potentially neoplastic and neoplastic oral lesions consisting of 29 cases of hyperplasia, 5 cases of dysplasia, and 19 cases of squamous cell carcinomas, as well as 16 oral specimens derived from healthy individuals. A highly sensitive nested polymerase chain reaction (PCR) assay was used, along with type-specific PCR, restriction fragment length polymorphism analysis, dot blotting, and nonisotopic in situ hybridization. Nested PCR revealed the presence of HPV DNA in 48 of the 53 (91%) pathologic samples analyzed, whereas none (0%) of the normal specimens was found to be infected. Positivity for HPV was independent of histology and the smoking habits of the analyzed group of patients. At least one "high risk" type, such as HPV 16, 18, and 33, was detected by type-specific PCR in 47 (98%) infected specimens, whereas only 1 (2%) squamous cell carcinoma was solely infected by a "low risk" type (HPV 6). HPV 16 was the prevailing viral type, being present in 71% of infected cases. Single HPV 16 and HPV 18 infections were confirmed by restriction fragment length polymorphism. HPV 58 was detected by dot blotting in three hyperplastic lesions. HPV positivity and genotyping were further confirmed, and the physical status of this virus was evaluated by nonisotopic in situ hybridization. Diffuse and punctate signals, indicative of the episomal and integrative pattern of HPV infection, were observed for low-and high-risk types, respectively. Our findings are suggestive of an early involvement of high-risk HPV types in oral carcinogenesis.

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Section: Discussionsupporting

confidence: 89%

“High Risk” HPV Types Are Frequently Detected in Potentially Malignant and Malignant Oral Lesions, But Not in Normal Oral Mucosa

et al. 2000

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“…Increased AgNOR areas with increasing grades of malignancy have been reported in non-Hodgkins lymphoma,[11] in colonic tumors,[21] and melanocytic neoplasm of skin. [22] Similar findings have also been reported in oral cavity by Muzio et al .,[23] who found increased mean AgNOR area/nucleus in patient with moderate and severe dysplasia as compared to mild dysplasia. Cabrini et al .,[24] also reported increasing AgNOR area from normal oral epithelial to benign lesions and to SCC.…”

Section: Discussionsupporting

confidence: 79%

“…[6] The results of our study are consistent with findings of Muzio et al ., who found that the high value of AgNOR area in oral dysplasia could be a risk marker which could help in identifying the subgroup of lesions with worse prognosis. [23]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the efficacy of AgNOR as a proliferative marker in oral leukoplakia: A morphometric analysis

Garg

Raj

Chandra

2013

Natl J Maxillofac Surg

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Background:Silver stainable nucleolar organizer regions (AgNORs) are replicatory markers which may have a place in objectively characterizing dysplasia.Materials and Methods:A study of various morphometric parameters related to AgNORs was performed in basal and parabasal layers of normal human oral epithelium, nondysplastic leukoplakia, and dysplastic leukoplakia employing photomicrographs of silver stained paraffin embedded sections using image analysis, to assess the usefulness of these parameters in distinguishing dysplastic leukoplakia from nondysplastic oral leukoplakia.Results:Out of various mean AgNOR related parameters, AgNOR count, area, perimeter, and proportion were found to be higher in dysplastic leukoplakia as compared to nondysplastic leukoplakia. On statistical analysis, AgNOR count showed statistically significant differentiation between dysplastic and nondysplastic leukoplakia. While other parameters can distinguish normal oral epithelium from dysplastic and nondysplastic leukoplakia.Conclusion:To conclude, the AgNOR count is the most appropriate marker to differentiate between dysplastic and nondysplastic leukoplakia.

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“…This system is commonly used in pathology. [25][26][27][28] The operator performed the analysis on all the tissue blocks and was blinded to the patients' symptomatology. The video signal, which was proportional to the intensity, was digitized into 756 × 668 pixels with 24-bit color resolution.…”

Section: Pathology Protocol and Quantitative Measurementmentioning

confidence: 99%

Cerebral Ischemic Disease and Morphometric Analyses of Carotid Plaques

Swijndregt

Elbers

Moll

et al. 1999

Annals of Vascular Surgery

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Morphometric study of nucleolar organiser regions (AgNOR) in HPV-associated precancerous lesions and microinvasive carcinoma of the oral cavity

Cited by 20 publications

References 60 publications

“High Risk” HPV Types Are Frequently Detected in Potentially Malignant and Malignant Oral Lesions, But Not in Normal Oral Mucosa

“High Risk” HPV Types Are Frequently Detected in Potentially Malignant and Malignant Oral Lesions, But Not in Normal Oral Mucosa

Evaluation of the efficacy of AgNOR as a proliferative marker in oral leukoplakia: A morphometric analysis

Cerebral Ischemic Disease and Morphometric Analyses of Carotid Plaques

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