Abstract:Morphometric data were collected on 15 characters in adults of the species of the Pissodes strobi complex (P: nemorensis (Germar), P: schwarzi Hopkins, P: strobi (Peck), and P: terminalis Hopping). Data were examined using stepwise discriminant analysis to determine if all characters contribute significantly to the diagnosis of taxa, and their relative amounts of resolving power were illuminated using canonical variates analysis. Discriminant functions were generated to diagnose species. Separate discriminant … Show more
“…Sixty-eight female and 72 male Pissodes specimens collected from nine sites across South Africa (Online Resource 1) were characterised by measuring 15 morphometric characters (Online Resource 5) used to discern members within the P. strobi complex (Williams & Langor, 2002). The specimens were measured using calibrated AxioCam ICc 5 Zeiss camera attached to a Carl Zeiss Stemi 2000 stereo microscope.…”
Section: Morphometric Measurementsmentioning
confidence: 99%
“…Morphometric data measured in the present study and those reported by Williams and Langor (2002) were compared by factor analysis (FA) in order to describe morphometric similarity/difference of the South…”
Section: Morphometric Data Analysismentioning
confidence: 99%
“…All the morphometric characters used by Williams and Langor (2002) except "width of elytra at anterior margin of declivity (EA)" and "depth of mesothorax at deepest point (MD)" were used in our FA (i.e., 13 measured and three calculated ratio characters in total). Prior to FA, original datasets for North American species were simulated based on the published mean, standard error and range values (assuming normal distributions).…”
Section: Morphometric Data Analysismentioning
confidence: 99%
“…Measurements of all morphometric characters, except snout apex proportion (SA), were the highest in both females and males of the Pissodes sp. in South Africa (Online Resource 5) compared to published morphometries of other members of the P. strobi complex (Williams & Langor, 2002). The depth of pronotum along posterior margin (PD) and length of snout between apex and eye margin at mid-height (SL) were found remarkably longer in both the females and males of the Pissodes sp.…”
Section: Identification Of the Pissodes Specimens Introduced In Southmentioning
confidence: 99%
“…The depth of pronotum along posterior margin (PD) and length of snout between apex and eye margin at mid-height (SL) were found remarkably longer in both the females and males of the Pissodes sp. in South Africa (Online Resource 5) than other members of the P. strobi complex (Williams & Langor, 2002). …”
Section: Identification Of the Pissodes Specimens Introduced In Southmentioning
Department, Uruguay) are acknowledged for providing dead insect specimens. We also thank the two anonymous reviewers for their constructive suggestions and comments.
AbstractDuring the first half of the twentieth century, two accidental cases of introduction of Pissodes weevils were recorded from the southern hemisphere. The weevils in South Africa were identified as the deodar weevil (Pissodes nemorensis) and those in South America as the small banded pine weevil (Pissodes castaneus). Wide distribution of the two species in their invasive range, general difficulty in identifying some Pissodes spp., and the varying feeding and breeding behaviours of the species in South Africa has necessitated better evidence of 1 species identity and genetic diversity of both species and population structure of the species in South Africa.Barcoding and the Jerry-to-Pat region of the COI gene were investigated. Morphometric data of the South African species was analysed. Our results confirmed the introduction of only one Pissodes species of North American origin to South Africa. However, this species is not P. nemorensis, but an unrecognized species of the P. strobi complex or a hybrid between P. strobi and P. nemorensis. Only P. castaneus, of European origin, was identified from South America. We identified ten mitochondrial DNA haplotypes from South Africa with evidence of moderate genetic structure among geographic populations. Terminal leader and bole-feeding weevils did not differ at the COI locus. A single haplotype was identified from populations of P. castaneus in South America. Results of the present study will have implications on quarantine, research and management of these insect species.
“…Sixty-eight female and 72 male Pissodes specimens collected from nine sites across South Africa (Online Resource 1) were characterised by measuring 15 morphometric characters (Online Resource 5) used to discern members within the P. strobi complex (Williams & Langor, 2002). The specimens were measured using calibrated AxioCam ICc 5 Zeiss camera attached to a Carl Zeiss Stemi 2000 stereo microscope.…”
Section: Morphometric Measurementsmentioning
confidence: 99%
“…Morphometric data measured in the present study and those reported by Williams and Langor (2002) were compared by factor analysis (FA) in order to describe morphometric similarity/difference of the South…”
Section: Morphometric Data Analysismentioning
confidence: 99%
“…All the morphometric characters used by Williams and Langor (2002) except "width of elytra at anterior margin of declivity (EA)" and "depth of mesothorax at deepest point (MD)" were used in our FA (i.e., 13 measured and three calculated ratio characters in total). Prior to FA, original datasets for North American species were simulated based on the published mean, standard error and range values (assuming normal distributions).…”
Section: Morphometric Data Analysismentioning
confidence: 99%
“…Measurements of all morphometric characters, except snout apex proportion (SA), were the highest in both females and males of the Pissodes sp. in South Africa (Online Resource 5) compared to published morphometries of other members of the P. strobi complex (Williams & Langor, 2002). The depth of pronotum along posterior margin (PD) and length of snout between apex and eye margin at mid-height (SL) were found remarkably longer in both the females and males of the Pissodes sp.…”
Section: Identification Of the Pissodes Specimens Introduced In Southmentioning
confidence: 99%
“…The depth of pronotum along posterior margin (PD) and length of snout between apex and eye margin at mid-height (SL) were found remarkably longer in both the females and males of the Pissodes sp. in South Africa (Online Resource 5) than other members of the P. strobi complex (Williams & Langor, 2002). …”
Section: Identification Of the Pissodes Specimens Introduced In Southmentioning
Department, Uruguay) are acknowledged for providing dead insect specimens. We also thank the two anonymous reviewers for their constructive suggestions and comments.
AbstractDuring the first half of the twentieth century, two accidental cases of introduction of Pissodes weevils were recorded from the southern hemisphere. The weevils in South Africa were identified as the deodar weevil (Pissodes nemorensis) and those in South America as the small banded pine weevil (Pissodes castaneus). Wide distribution of the two species in their invasive range, general difficulty in identifying some Pissodes spp., and the varying feeding and breeding behaviours of the species in South Africa has necessitated better evidence of 1 species identity and genetic diversity of both species and population structure of the species in South Africa.Barcoding and the Jerry-to-Pat region of the COI gene were investigated. Morphometric data of the South African species was analysed. Our results confirmed the introduction of only one Pissodes species of North American origin to South Africa. However, this species is not P. nemorensis, but an unrecognized species of the P. strobi complex or a hybrid between P. strobi and P. nemorensis. Only P. castaneus, of European origin, was identified from South America. We identified ten mitochondrial DNA haplotypes from South Africa with evidence of moderate genetic structure among geographic populations. Terminal leader and bole-feeding weevils did not differ at the COI locus. A single haplotype was identified from populations of P. castaneus in South America. Results of the present study will have implications on quarantine, research and management of these insect species.
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