Morphometrical Analysis of Sex and Strain Differences in the Mouse Nephron.

Yabuki, Akira; Suzuki, Syusaku; Matsumoto, Mitsuharu; Nishinakagawa, Hayao

doi:10.1292/jvms.61.891

Cited by 24 publications

(35 citation statements)

References 11 publications

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“…Moreover, changes in diameter of renal corpuscles may have been affected not only by changes in glomerular size, but also by changes in height of the parietal layer of glomerular capsules. Actually, the presence of a cuboidal parietal layer in the glomerular capsules represents a well-known morphological feature of the adult male mouse kidney, and the relationship between the diameter of renal corpuscles and the height of the parietal layer of glomerular capsules has already been suggested [18]. The present study failed to demonstrate any association between increased renal corpuscle diameter and glomerular damage in the course of age-dependent nephropathy.…”

contrasting

confidence: 71%

Morphometric Study of Gender Differences with Regard to Age-Related Changes in the C57BL/6 Mouse Kidney

et al. 2006

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confidence: 71%

Morphometric Study of Gender Differences with Regard to Age-Related Changes in the C57BL/6 Mouse Kidney

et al. 2006

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“…The parietal layer of the glomerular capsule commonly consists of squamous cells. However, in the laboratory mouse, morphological features of this layer differ among the sexes, with males exhibiting cuboidal cells [15,17,29]. Except for mice, sex-based morphological differences in the parietal layer of the glomerular capsule had not been evaluated previously.…”

Section: Discussionmentioning

confidence: 99%

“…Morphometric analysis: Randomized morphometric analysis was performed according to previous methods by using PAS-stained sections (5 sections/animal): 1) diameter of the cortical renal corpuscles which were located in the superficial and middle areas of cortex [29], 2) diameter of the juxtamedullary renal corpuscles which were located in the juxtamedullary area of cortex [29], 3) percentage of the renal corpuscles with a cuboidal parietal layer (CPL) [29], 4) number of nuclei in proximal convoluted tubules (PCTs) per unit area of cortex (100,000 µm 2 ) [29], 5) semi-quantitative score of the PAS-positive granules in PCTs, and 6) semi-quantitative score of the PAS-positive granules in proximal straight tubules (PSTs). Semi-quantitative analysis of the PAS positive granules were performed as follows.…”

Section: Animalsmentioning

confidence: 99%

Rodent Renal Structure Differs among Species

et al. 2006

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ABSTRACT. In the present study, we histologically and morphometrically investigated species differences in renal structure using laboratory rodents (mice, gerbils, hamsters, rats, and guinea pigs). Morphometric parameters were as follows, 1) diameter of the cortical renal corpuscles, 2) diameter of the juxtamedullary renal corpuscles, 3) percentage of the renal corpuscles with a cuboidal parietal layer, 4) number of nuclei in proximal convoluted tubules (PCTs) per unit area of cortex, 5) semi-quantitative score of the periodic acid-Schiff (PAS) -positive granules in PCTs, and 6) semi-quantitative score of the PAS-positive granules in proximal straight tubules (PSTs). Significant species differences were detected for each parameter, and particularly severe differences were observed in the PAS-positive granules of PCTs and PSTs. Granular scores varied among species and sexes. Vacuolar structures that did not stain with PAS or hematoxylin-eosin were observed in the renal proximal tubules. The appearance and localization of these vacuolar structures differed remarkably between species and sexes. KEY WORDS: morphology, morphometry, rodent kidney, sex difference, species specific difference.J. Vet. Med. Sci. 68(5): 439-445, 2006 A thorough understanding of the complex structures of the mammalian kidney provides a basis for comprehending the multitude of functional characteristics of this organ in both healthy and diseased states. The structure of this organ is closely related to its functions including production of urine, regulation of osmolarity, pH, body fluid composition and blood pressure, and metabolism of various substances. Many reports have addressed species-specific differences in mammalian kidneys [1,3,9,10,14,20,25]. In laboratory rodents, glomerular filtration rate (GFR) [10], activities of some dehydrogenases in the macula densa [14], and maxim urine-concentrating capacity [20] were found to differ among species. However, because of a lack of comparative studies, morphological differences in renal structure remain unclear. In the present study, to clarify species differences in renal structure of laboratory rodents, we comparatively investigated the kidneys of mice, gerbils, hamsters, rats, and guinea pigs using a morphometric approach. MATERIALS AND METHODS Animals:Male and female DBA/2CrSlc mice (n=5/each sex), MON/JmsGbsSlc gerbils (n=3/each sex), Slc:Syrian hamsters (n=3/each sex), F344/NSlc rats (n=4/each sex) and Slc:Hartley guinea pigs (n=3/each sex) were used in the present study. All animals were housed in an open system room with a one-way airflow system (temperature 22 ± 1°C; humidity 55 ± 10%; light period 07:00 to 19:00; ventilation 12 cycles/hr), and were given an autoclaved commercial diet (CE-2; Japan CLEA Inc., Tokyo, Japan) and tap water ad libitum. The present study was performed in accordance with the Guidelines for Animal Experimentation of Kagoshima University, Japan. All animals were sacrificed at 90 days of age by exanguination of the carotid arteries under anesthesia using a m...

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“…In our recent studies, we have demonstrated strain and sex-based morphological varieties of mouse kidneys [6,7]. In these studies, we investigated kidney morphology in male and female mice from five strains (ICR, BALB/c, C3H/He, C57BL/6, and DBA/2), and identified femalespecific cytoplasmic granules in the proximal straight tubules (PSTs) [7].…”

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Age-Dependent Changes in the Kidney Morphology of Female DBA/2 Mice

2009

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Abstract:A female DBA/2 mouse is characterized by the presence of abundant cytoplasmic granules in the renal tubules. In the present study, the morphometrics of kidneys from female DBA/2 mice at 5, 15, 18, 21, and 24 months of age were investigated to determine the agedependent renal changes in this mouse strain. Glomerular and tubulointerstitial disease progressed with age, and the semiquantitative scores of these lesions showed significant increases. Granules were observed in the proximal straight tubules and no changes were observed in their localization, fine structure, and quantitative scores. It was concluded that the tubular cytoplasmic granules in the female DBA/2 mouse were not affected by agedependent functional reduction of the kidney.

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Morphometrical Analysis of Sex and Strain Differences in the Mouse Nephron.

Cited by 24 publications

References 11 publications

Morphometric Study of Gender Differences with Regard to Age-Related Changes in the C57BL/6 Mouse Kidney

Morphometric Study of Gender Differences with Regard to Age-Related Changes in the C57BL/6 Mouse Kidney

Rodent Renal Structure Differs among Species

Age-Dependent Changes in the Kidney Morphology of Female DBA/2 Mice

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