Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus

Maifrino, Laura Beatriz Mesiano; Prates, José Carlos; De-Souza, Romeu Rodrigues; Liberti, Édson Aparecido

doi:10.1590/s0100-879x1997000500009

Cited by 14 publications

(13 citation statements)

References 12 publications

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“…The myenteric plexus as an incompact and irregular plexo-ganglionic network with different expression of AChE activity was observed in the wild mouse (Calomys callosus), hamster, rat and guinea pig. In these species the wall of the oesophagus contained striated muscles [16][17][18][19]. A similar level of development of the Auerbach plexus was found in chinchilla.…”

Section: Discussionsupporting

confidence: 63%

“…An AChE-positive network containing first-order (thicker) and second-order fibres with neurons in nodes was observed in wild mouse (Calomys callosus) [16]. Varicosities-forming fluorescent fibres represented the adrenergic structures of this plexus visible both in ganglia and in the connecting bundles.…”

Section: Discussionmentioning

confidence: 92%

“…Macromorphological investigations of the gastric Auerbach plexus in wild mouse (Calomys callosus), cat, opossum and rabbit revealed networks with a characteristic shape of meshes, formed by nodes with agglomerations of neurons connected to bundles of fibres [8,16,20].…”

Section: Discussionmentioning

confidence: 99%

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Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Nowak

2013

Folia Histochem Cytobiol.

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Using histochemical and histological techniques the morphology and topography of the autonomic plexuses of the oesophagus and stomach in chinchilla (Chinchilla laniger) were investigated. Myenteric and submucosal plexuses were stained for cholinergic and adrenergic structures. Our results showed that the cholinergic structures were formed by networks of various shape and of different size of the neuron agglomerates and nerve fibres. Adrenergic fibres were also found. Density of these structures varied in different parts of the investigated organs. This study is the first attempt to localize the intramural autonomic plexuses in chinchilla.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 92%

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Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Nowak

2013

Folia Histochem Cytobiol.

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“…7,9,11,12 In the present investigation, this technique has shown that the NADH reaction can be used to stain neurons in the tissues taken from chagasic animals. Using this technique, it was observed that in this experimental group there were areas in which the ganglion neurons were absent, in some areas no plexus was seen, but in others an anatomically normal plexus was seen.…”

Section: Discussionmentioning

confidence: 65%

Morphometry and acetylcholinesterase activity of the myenteric neurons of the mouse colon in the chronic phase of experimental Trypanosoma cruzi infection.

Maifrino¹,

Liberti²,

Watanabe³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. The myenteric plexus of the proximal colon, midcolon, and distal colon was studied in mice chronically infected with the Y strain of Trypanosoma cruzi by means of histochemical methods for NADH-diaphorase and acetylcholinesterase (AChE) on whole mount preparations. Ganglia of infected mice displayed an irregular distribution, with neurons severely altered in form and were found side by side with slightly degenerated or morphologically normal ones. Significant reductions of at least 36% in the numbers of neurons were recorded in all regions of the colons of infected animals, especially in the distal colon where the neuron number decreased by more than 44%. Measurements of neuron size suggest that the neuronal destruction caused by T. cruzi affected the medium and large neurons. The small neurons apparently were not affected by the infection. The histochemical demonstration of AChE by the direct coloring copper ferrocyanide method showed that in the control animals, most of the neurons of the plexus displayed AChE activity in the cytoplasm although the neurons showed different reaction intensities. The AChE activity was also present, but at a lower intensity, in the myenteric plexus of the colons of infected animals. These results suggest that the T. cruzi infection affects some categories of neurons and implies that some particular enteric neurotransmitter systems could be affected and the potency of their action upon intestinal function consequently reduced.The digestive form of Chagas' disease involves denervation of the myenteric plexus, which influences disturbances of motility and absorption. 1 The extent of the neurolysis will determine the severity of the sequelae and the patient's future degree of clinical adaptation. 2 Studies involving the myenteric plexus of the colon of animals chronically infected with Trypanosoma cruzi have been carried out and have demonstrated a reduction of 34-37% of the neuronal population. 3,4 Okumura 5 found that 65% of the ganglion neurons of the mouse colon may be destroyed in experimentally infected mice. These studies were done in histologic sections made in the plane of the plexus. However, these sections rarely pass through more than a few ganglia simultaneously, while whole mount preparations, in which the muscle layers are stretched on a plane, can display the whole plexus. 6 Furthermore, the use of whole mount preparations presents the myenteric plexus as a monolayer of nerve cells, and it has therefore been possible, in parallel with neuron counts, to make measurements of neuron somata of unsectioned nerve cells. 7 Therefore, in this study, we used laminar preparations of the mouse colon to 1) quantify the loss of myenteric neurons that occurred with T. cruzi infection, 2) see whether any particular size grouping of neurons is affected, and 3) determine the activity of acetylcholinesterase (AChE) in the myenteric plexus of the mouse colon chronically infected with T. cruzi. MATERIALS AND METHODSTwenty male Swiss mice weighing 20-25 g (13-15 weeks old) were ...

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“…In a previous study, we examined the morphology of the myenteric plexus of the digestive tract of Calomys callosus (10) in view of the fact that the different effects of chagasic infection on the enteric plexuses of different species may be due to the structure of the plexuses.…”

Section: Introductionmentioning

confidence: 99%

Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus

Souza

Liberti

De-Souza

1998

Braz J Med Biol Res

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The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy.The plexus contains ( x _ ± SD) 7,488 ± 293 neurons/cm 2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of large nerve bundles and in a secondary meshwork of finer nerve bundles. At the ultrastructural level, the ganglia are irregular in shape and covered with fibroblastlike cells. The nucleoplasm of the neurons is finely granular with a few condensations of chromatin attached to the nuclear envelope. In the neuropil numerous varicosities filled with vesicles of different size and electron densities are seen. The pre-and post-synaptic membrane thickenings are asymmetric. Characteristic glial cells with oval nuclei and few organelles are numerous. These data provide a detailed description of this submucosal meshwork.

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Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus

Cited by 14 publications

References 12 publications

Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Morphometry and acetylcholinesterase activity of the myenteric neurons of the mouse colon in the chronic phase of experimental Trypanosoma cruzi infection.

Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus

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