Morphometry As a Diagnostic Tool for Potentially Malignant Lesions

Christopher,; Murthy, Sarvani; Singh, Shivani; A, Cp; Sk, Shivaram; Neethupriya,

doi:10.7860/jcdr/2015/15838.6959

Cited by 6 publications

(5 citation statements)

References 8 publications

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“…In earlier research, OSSC patients had a statistically significant increase in nuclear length, nuclear part, cell part, and nuclear-cytoplasmic percentage in oral leukoplakia and oral verrucous carcinoma compared to normal mucosa. 16,17 In this research, the highest occurrence of OSSC was observed in the age group of 45-64 years, followed by 25-44 years, with a mean age of 63 which was consistent with previous report. 18 The male to female ratio was found to be 2:1 in this study, which was consistent with earlier research that revealed a higher incidence of tobacco use in males than females due to easier access to tobacco products.…”

Section: Discussionsupporting

confidence: 91%

Age and gender demographic and statistical analysis in oral squamous cell carcinoma in Eastern India

Bhuyan¹,

Bhuyan²,

Panda³

et al. 2023

JAMS

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Background: Oral squamous cell carcinoma (OSCC) continues to dominate all the oral cancer cases with premalignant disorders, which can also be predictable at the pre-clinical phase of malignancy. Chewing behaviors such as betel quid, areca nut& tobacco are all major risk factors. The majority of OSCC patients present with advanced disease and a poor prognosis. Despite the high prevalence of this cancer, there is a scarcity of rigorous data from Eastern India on its relationship with known risk factors. Objectives: The aim of this study was to evaluate the features at specific ages and gender that could play a major role in the development of oral squamous cell carcinoma. Materials and methods: Demographics and habitual behavior were both subjected to expressive analysis. In patients with oral squamous cell carcinoma, categorical and continuous variables regarding gender were scrutinized. Results: Age with gender cross tabs results reflects the maximum patients 245 (57.24%) were female at the age of 45-64, followed by the same age group male 480 (51.39%). The highest 654 cancer sites were found in the buccal mucosal region, and we found the total number of 349 (53.36%) were in the same age group between 45-64 years. Precisely we got the age mean±SD of 61±9 male and 64±12 female OSSC patients with statistical significance of <0.001 respectively. Conclusion: Finally, the majority of OSCC cases in our area of eastern India were female patients who had been using gudhakhu for a long time and it was found correlating with site of OSCC in female.

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Section: Discussionsupporting

confidence: 91%

Age and gender demographic and statistical analysis in oral squamous cell carcinoma in Eastern India

Bhuyan¹,

Bhuyan²,

Panda³

et al. 2023

JAMS

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“…Multiple studies have reported using Feulgen staining to study nuclear and cellular morphometry and chromatin frameworks in oral cytosmears or paraffin-embedded tissue sections of various potentially malignant lesions, such as oral hyperplasia, dysplasia and OSCC. [6][7][8][14][15][16][17][18] In the present study, different mean mitotic counts were calculated using different stains, with a significantly greater frequency of MFs observed among samples stained with Feulgen stains compared to those stained with 1% crystal violet, regardless of OSCC grade. Similarly, Rao et al also noted a significant increase in the number of MFs in Feulgenstained sections as compared to 1% crystal violetstained samples.…”

Section: Discussionmentioning

confidence: 70%

“…the cellular area [CA], cellular perimeter [CP], nuclear area [NA] and nuclear perimeter [NP]) as well as the N/C ratio have proven useful in differentiating between normal tissues and potentially malignant disorders such as leukoplakia, lichen planus and oral squamous cell carcinoma (OSCC). 7 Previous studies have deduced that computer-assisted image analysis allows for the assessment of large numbers of specimens with high reproducibility as well as sensitivity and accuracy rates exceeding 99%. 8,9 This method also allows for precise measurement of the size, shape, organisation and quantity of cells and their nuclei, while reducing interobserver discrepancies, thus increasing the reliability of accurate histopathological grading of the lesion.…”

mentioning

confidence: 99%

Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains

Kesarkar

Tamgadge

Peirera

et al. 2018

Sultan Qaboos Univ Med J

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“…OSCCs are preceded by potentially malignant lesions such as erythroplakia, leukoplakia, speckled leukoplakia, or proliferative verrucous leukoplakia, but it is not uncommon to see them arise in apparently normal oral mucosa. [ 1 ] OSCCs are characterized by multitudes of etiopathogenetic agents with tobacco and alcohol as main offenders. DNA viruses, especially human papillomavirus, have been documented to play a role in the initiation or development of these lesions.…”

Section: Introductionmentioning

confidence: 99%

A comparative immunohistochemical study of presence and distribution pattern of stromal myofibroblast in oral dysplasia and in different grades of oral squamous cell carcinoma

Bhattacharjee

Girish

Murgod

et al. 2018

J Int Soc Prevent Communit Dent

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Aims and Objectives:Squamous cell carcinoma (SCC) is a common oral malignancy with a poor survival rate. Early tumorigenesis is marked by transdifferentiation of fibroblasts to myofibroblasts (MFs), which is supported by growth factors and cytokines expressed by tumor cells. The expression of alpha-smooth muscle actin (αSMA) marker correlates with the activation of MFs. This study was undertaken to compare the frequency and distribution of αSMA immunoexpression in oral epithelial dysplasia (OED) and OSCC.Materials and Methods:This study was conducted on samples collected from patients with oral epithelial dysplasia and oral SCC who visited Rajarajeswari Dental College and Hospital, Bengaluru. Tissue sections were subjected to Immunohistochemistry using αSMA marker, and cells were counted. The obtained data was subjected to Kruskal–Wallis test and Mann–Whitney U-test.Results:On performing Kruskal–Wallis test and Mann–Whitney U-test between the three groups (normal oral mucosa, OED, and OSCC) statistically significant result was found in the frequency between OED and OSCC and between normal tissue and OSCC. On comparing the distribution pattern, statistically significant result was found between OED and OSCC and between normal tissue and OSCC.Conclusion:The expression of MFs increases as the disease progresses from high-grade epithelial dysplasia to invasive OSCC. Poorly differentiated SCC showed more attendance of positive MFs in the stroma than other grades of OSCC. The rise in the number of αSMA-positive MFs and change in distribution pattern in OSCC can be associated with tumor invasive characteristics. Thus, the proliferation of MFs may be used as a stromal marker of premalignancy and malignancy.

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Morphometry As a Diagnostic Tool for Potentially Malignant Lesions

Cited by 6 publications

References 8 publications

Age and gender demographic and statistical analysis in oral squamous cell carcinoma in Eastern India

Age and gender demographic and statistical analysis in oral squamous cell carcinoma in Eastern India

Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains

A comparative immunohistochemical study of presence and distribution pattern of stromal myofibroblast in oral dysplasia and in different grades of oral squamous cell carcinoma

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