Morphometry of the midgut epithelium of Diatraea saccharalis Fabricius, 1794 (Lepidoptera) parasitized by Cotesia flavipes Cameron, 1891 (Hymenoptera)

Pinheiro, Daniela O.; Silva, Reinaldo J.; Gregório, Elisa Aparecida

doi:10.1016/j.jip.2006.05.004

Cited by 9 publications

(10 citation statements)

References 9 publications

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“…The significant increase in the length of the midgut, as found for M. quadrifasciata anthidioides, has already been reported in Hemiptera (Billingsley 1988) and Lepidoptera (Pinheiro et al 2003, Pinheiro & Gregório 2006, Levy et al 2009. It is known that in stingless bees, the increase in midgut size during the larval stage is due to the growth of digestive cells (Cruz-Landim & Mello 1970), and to the increase in cell number due to proliferation and differentiation of regenerative cells (Serrão & CruzLandim 2000, Martins et al 2005.…”

Section: Discussionmentioning

confidence: 73%

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Cruz¹,

Araujo²,

Dolder³

et al. 2011

Neotrop. entomol.

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In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

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Section: Discussionmentioning

confidence: 73%

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Cruz¹,

Araujo²,

Dolder³

et al. 2011

Neotrop. entomol.

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“…Many works on peritrophic membrane secretion in Lepidoptera larvae suggest that its constituents are produced by columnar cells, preferentially in the anterior midgut region of insects (Santos et al, 1984). A previous work on parasitized D. saccharalis showed that the columnar cells present cytoplasmic and nuclear increase in the posterior midgut region of D. saccharalis larvae, due to parasitism, verified by means of morphometry (Pinheiro et al, 2006). Thus the finding of greater mitochondrial alteration in columnar cells of the anterior midgut region indicates that this region is the more affected by parasitism.…”

Section: Discussionmentioning

confidence: 83%

“…Previous morphometric works show that these cells did not suffer alteration in cytoplasmic or nuclear volume during the parasitism process, in both midgut regions (Pinheiro et al, 2006), which was attributed to the high variability in the morphology of these cells, existent even in non-parasitized larvae . This variability of regenerative cells must be related to their function along the midgut; it is known that these cells may be differentiated in other epithelial cell types, presenting a high index of morphological variability (Lehane and Billingsley, 1996;Illa-Bochaca and Montuenga, 2006).…”

Section: Discussionmentioning

confidence: 87%

“…These microvilli are related to the active transport mechanisms of K + ions from the hemolymph to the intestinal lumen, in cooperation with the adjacent columnar cells (Anderson and Harvey, 1966;Harvey et al 1983;Moffett et al, 1995;Lehane and Billingsley, 1996;Zeiske et al, 2002). The greater intensity of mitochondrial lesions in the anterior region, due to parasitism, is not reflected in the morphometry of goblet cells (Pinheiro et al, 2006), since there are cytoplasmic and nuclear increases in both midgut regions of D. saccharalis parasitized larvae.…”

Section: Discussionmentioning

confidence: 90%

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Mitochondria in the midgut epithelial cells of sugarcane borer parasitized by Cotesia flavipes (Cameron, 1891)

2010

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The sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) has been controlled by Cotesia flavipes (Hymenoptera: Braconidae); however, very little is known about the effect of the parasitism in the host organs, including the midgut. This work aims to verify mitochondrial alteration in the different midgut epithelial cells of D. saccharalis parasitized by C. flavipes. Midgut fragments (anterior and posterior region) of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. The mitochondria of midgut epithelial cell in the parasitized larvae exhibit morphological alteration, represented by matrix rarefaction and vacuolisation. These mitochondrial alterations are more pronounced in the anterior midgut region during the parasitism process, mainly in the columnar cell.Keywords: midgut, Diatraea saccharalis, mitochondria, Cotesia flavipes, epithelial cell. Mitocôndria nas Células Epiteliais do Intestino Médio da Broca-da-cana Parasitada por Cotesia flavipes ResumoDiatraea saccharalis (Lepidoptera: Crambidae), broca da cana-de-açúcar, tem sido controlada por Cotesia flavipes (Hymenoptera: Braconidae); pouco se sabe sobre o efeito do parasitismo nos diferentes órgãos do inseto hospedeiro, principalmente no intestino médio. O objetivo desse trabalho foi verificar as alterações mitocondriais das diferentes células epiteliais do intestino médio de larvas de D. saccharalis parasitadas por C. flavipes. Fragmentos do intestino médio (regiões anterior e posterior) de larvas de D. saccharalis não-parasitadas e parasitadas foram processados para microscopia eletrônica de transmissão. As mitocôndrias das células epiteliais do intestino médio de larvas parasitadas exibem alterações, especialmente rarefação e vacuolização da matriz, que foram mais pronunciadas nas células epiteliais da região anterior do intestino médio na vigência do parasitismo, em especial nas células colunares.Palavras-chave: intestino médio, Diatraea saccharalis, mitocôndria, Cotesia flavipes, célula epitelial.

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“…Despite the importance of this insect, there are few works on its internal morphology, especially in reference to the midgut. The existent descriptions reference the ultrastructural differences of columnar cells , and morphometric distinctions in different cellular types (Pinheiro et al , 2006, besides biochemical differences of enzymatic activity (Azevedo et al 2003). However, none of these studies characterize morphological variations in the different cellular types along the midgut of the sugarcane borer.…”

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confidence: 99%

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Pinheiro¹,

Quagio-Grassiotto²,

Gregório³

2008

Neotrop. entomol.

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Diferenças Morfológicas Regionais das Células Epiteliais ao Longo do Intestino Médio de Larvas deDiatraea saccharalis Fabricius (Lepidoptera: Crambidae)RESUMO -A broca da cana, Diatraea saccharalis Fabricius, é uma praga da cana-de-açúcar e outras plantações. O objetivo deste trabalho foi caracterizar variações morfológicas nas células epiteliais (colunares, caliciformes e regenerativas) ao longo do intestino médio de larvas de D. saccharalis. Fragmentos do intestino médio (anterior, mediano e posterior) foram fi xados e processados para microscopia de luz e eletrônica de varredura. Existem diferenças morfológicas citoquímicas e ultraestruturais nas células epiteliais, dependendo da sua localização no intestino médio. A superfície apical de algumas células colunares exibe projeções citoplasmáticas que aumentam em número e volume da região anterior para a posterior do intestino médio. Existe aumento dos grânulos PAS-positivos (Reação de Schiff) no citoplasma apical das células colunares e regenerativas, da região anterior para a posterior. A câmara das células caliciformes, na região anterior do intestino médio, mostra seu ápice estreito, enquanto que na posterior essa porção da câmara é alargada; as evaginações citoplasmáticas da câmara são pequenas e fi nas no ápice, sendo numerosas, longas e mais espessas na porção basal. Os resultados sugerem que o intestino médio da broca da cana apresenta duas regiões morfologicamente distintas, a anterior e posterior; a região mediana é uma região de transição.PALAVRAS-CHAVE: Morfologia, broca da cana, histoquímica, microscopia eletrônica de varredura, microscopia de luz ABSTRACT -The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fi xed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions.There is an increase in the amount of PAS-positive (Periodic Acid-Schiff Reaction) granules detected in the cytoplasm of both the columnar and regenerative cells, from the anterior to the posterior region. The goblet cell apical surface is narrow in the anterior region, and enlarged in the posterior midgut; the chamber's cytoplasm extrusion are small and thin at the apical cavity surface, being thicker, longer and more numerous at the basal portion of the cavity. Our results suggest that the sugarcane borer midgut has two morphologically different regions, the anterior and the posterior; the middle region is a transitional region.

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Morphometry of the midgut epithelium of Diatraea saccharalis Fabricius, 1794 (Lepidoptera) parasitized by Cotesia flavipes Cameron, 1891 (Hymenoptera)

Cited by 9 publications

References 9 publications

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis

Mitochondria in the midgut epithelial cells of sugarcane borer parasitized by Cotesia flavipes (Cameron, 1891)

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

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