Morphophysiological characterization of Ceratocystis fimbriata isolates from yerba mate

Brito, Nícolas Manarim de; Duarte, Henrique da Silva Silveira; Bühner, Caroline de Bastos; Auer, Celso Garcia; Santos, Á. F. dos

doi:10.1590/0103-8478cr20200579

Cited by 8 publications

(10 citation statements)

References 4 publications

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“…[ 75 , 77 , 78 ], Ficus carica [ 75 , 79 ], Punica granatum [ 80 , 81 ], Colocasia esculenta [ 80 , 82 , 83 ], Theobroma cacao [ 11 ], Gmelina arborea [ 84 , 85 ], Syngonium sp. [ 86 , 87 ] and Ilex paraguariensis [ 88 , 89 ]. In contrast, C. cacaofunesta has only been described in Theobroma cacao [ 90 ] and in species from the genus Herrania sp.…”

Section: Discussionmentioning

confidence: 99%

Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata

et al. 2023

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Theobroma cacao is one of the main crops of economic importance in the world as the source of raw material for producing chocolate and derivatives. The crop is the main source of income for thousands of small farmers, who produce more than 80% of the world’s cocoa supply. However, the emergence, re-emergence and proliferation of pathogens, such as Ceratocystis spp., the causative agent of Ceratocystis wilt disease and canker disease, have been affecting the sustainability of many crops. Fungal control is laborious, often depending on fungicides that are expensive and/or toxic to humans, prompting researchers to look for new solutions to counteract the proliferation of these pathogens, including the use of biological agents such as mycoviruses. In this study, we investigated the diversity of microorganisms associated with the T. cacao pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata with a focus on the virome using RNA sequencing data available in public databases. We used a comprehensive bioinformatics pipeline containing several steps for viral sequence enrichment and took advantage of an integrated assembly step composed of different assemblers followed by sequence similarity searches using NCBI nonredundant databases. Our strategy was able to identify four putative C. cacaofunesta viruses (hypovirus, sclerotimonavirus, alphapartitivirus and narnavirus) and six C. fimbriata viruses (three alphaendornaviruses, one victorivirus and two mitoviruses). All the viral sequences identified showed similarity to viral genomes in public databases only at the amino acid level, likely representing new viral species. Of note, we present the first report of viruses associated with the cacao pathogens C. cacaofunesta and C. fimbriata and the second report of viral species infecting members of the Ceratocystidaceae family. Our findings highlight the need for further prospective studies to uncover the real diversity of fungus-infecting viruses that can contribute to the development of new management strategies.

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Section: Discussionmentioning

confidence: 99%

Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata

et al. 2023

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“…The environment is of great importance in plant diseases. A study showed that the average optimum temperature for mycelial growth and sporulation of isolates of C. fimbriata of yerba mate are around 23°C (Brito et al, 2021). Because the plant growth chamber kept the temperature favourable to the pathogen throughout the experiment, it caused the disease to occur more quickly than in the greenhouse where there was no temperature control and there was great variation during the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…The experiment was performed in two different environmental conditions. One experiment was carried out in a plant growth chamber (InstalaFrio®) at the Laboratório de Epidemiologia para Manejo Integrado de Doenças, from the Universidade Federal do Paraná Curitiba, PR, Brazil, from October to November 2017, with manual irrigation, temperature of 23 ± 2°C, the optimal for Ceratocystis fimbriata mycelial growth (Brito et al, 2021), relative humidity of 80% and out during the spring season in a greenhouse at the Laboratório de Patologia Florestal of Embrapa Florestas, Colombo, PR, Brazil, from December 2017 to January 2018, without environmental control, which has a 50% shade cloth and manual irrigation. Daily data on temperature (°C) were obtained inside the experiments using maximum and minimum thermometer with a variation of −30 to 50°C (Walmur®).…”

Section: Aggressiveness Of Ceratocystis Fimbriata Isolatesmentioning

confidence: 99%

Aggressiveness and screening for host responses in yerba mate (Ilex paraguariensis) clones to a wilt disease caused by Ceratocystis fimbriata

et al. 2021

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Ceratocystis wilt of yerba mate (Ilex paraguariensis) caused by the fungus Ceratocystis fimbriata was observed in 2018 in commercial plantations located in São Mateus do Sul, PR and Arvorezinha, RS, Brazil, where mortality of the plants was up to 5%. Due to the need of control measures for this disease and to the lack of knowledge on the impact of this pathogen this new host, this study aimed to evaluate the aggressiveness of C. fimbriata isolates collected from yerba mate plantations and screen for host responses in yerba mate clones to Ceratocystis wilt. Two experiments were conducted in a completely randomized design, with 11 treatments (isolates) to assess aggressiveness of the isolates. Two experiments were conducted to assess resistance of yerba mate to C. fimbriata in a completely randomized design in a 6 × 2 factorial scheme (6 clones × 2 isolates) in two different environmental conditions (plant growth chamber and greenhouse). The isolates of C. fimbriata showed different levels of aggressiveness to yerba mate, and all evaluated clones were susceptible to Ceratocystis wilt. The plant growth chamber condition led to a faster death of the plants compared with greenhouse. This information will be useful to understand the pathosystem and seek strategies for controlling this disease in yerba mate.

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“…The knowledge of conditions that favours fungal growth and sporulation is important in microbiology and plant pathology. Temperature, relative humidity, and Hydrogen ion concentration are the main players in growth, reproduction and survival of the microorganisms (Brito et al, 2021). Physiological studies on fungi have played an important role in agriculture, industry, medicine and industrial production of vitamins (Madan and Thind, 1998).…”

Section: Introductionmentioning

confidence: 99%

Morphology, pathogenicity and physiology of Ceratocystis fimbriata causing black rot disease of Colocasia esculenta

Latif

2023

PAKJAS

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Arvi (Colocasia esculenta) is widely cultivated sweet and starchy corms as a staple food in most of the African and Asian countries. Black rot caused by Ceratocystis fimbriata is the most damaging post-harvest disease of Arvi in many countries. In June, 2019, the black rot disease symptoms were noticed on edible corms of Arvi for sale in different vegetable shops in Faisalabad, Pakistan. These symptoms were comprised of small, brown to greenish, slightly sunken spots or lesions which later on enlarged as black rot on the harvested corms. A comprehensive study was initiated to explore the pathogen associated with symptomatic corms. White to grey mycelia and black perithecia with long necks were observed within the lesions. The isolated pathogen was identified as Ceratocystis fimbriata based on morpho-physiological characteristics. Pathogenicity was proved on inoculated corms at 26±2°C and 70% relative humidity (RH) in plant growth chamber. The fungus was further tested for growth and reproduction physiology under different growth media and environmental parameters. A significant interaction between mycelial growth and culture media was observed. Highest mycelial growth (9 cm) of C. fimbriata was showed by Potato Carrot Agar (PCA) and lowest growth (0%) was observed on Water Agar (WA). There was a significant correlation between Temperature, RH, and hydrogen ion (pH) concentration responsible for the growth of C. fimbriata at (P < 0.01). The optimum fungal growth (8.75, 8.96, and 8.95 cm) was measured at 25°C, 70% RH, and pH 8 respectively. The paper may act as basic resource for future research on the epidemiology of this disease.

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Morphophysiological characterization of Ceratocystis fimbriata isolates from yerba mate

Cited by 8 publications

References 4 publications

Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata

Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata

Aggressiveness and screening for host responses in yerba mate (Ilex paraguariensis) clones to a wilt disease caused by Ceratocystis fimbriata

Morphology, pathogenicity and physiology of Ceratocystis fimbriata causing black rot disease of Colocasia esculenta

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