Morphotypes of<i>Prorocentrum lima</i>(Dinophyceae) from Hainan Island, South China Sea: morphological and molecular characterization

Zhang, Hua; Li, Yang; Cen, Jingyi; Wang, Hua-Long; Cui, Lei; Dong, Yan; Lü, Songhui

doi:10.2216/15-8.1

Cited by 41 publications

(54 citation statements)

References 47 publications

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“…4) were previously observed by Nagahama et al (2011). The fourth P. lima sub-clade (sub-clade C) observed in the present study consisted of strains from the South China Sea described by Zhang et al (2015) and a sequence named as Prorocentrum arenarium (strain VGO776) (Fig. 4).…”

Section: Discussionsupporting

confidence: 75%

“…5), with strains from the Atlantic, Indian and Pacific Oceans. Zhang et al (2015) also reported higher evolutionary rates for ITS in relation to LSU loci when Prorocentrum lima sequences were compared. Nagahama et al (2011) examined the intraspecific diversity of P. lima and concluded that the ITS sequences were also more variable and presented a higher resolution than the SSU rDNA sequences.…”

Section: Discussionmentioning

confidence: 88%

“…Cell size was within the range reported for the species. Zhang et al (2015) recently reported high morphological variability among P. lima isolates from the South China Sea and characterized five morphotypes that were separated based on ITS and LSU rDNA sequences. The strains isolated from Brazil are morphologically similar to the morphotype 2 of Zhang et al (2015).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Nascimento

Salgueiro

Menezes³

et al. 2016

Harmful Algae

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Nascimento

Salgueiro

Menezes³

et al. 2016

Harmful Algae

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“…The Malaysian strain of P. lima (SS0905) was clustered with strains of P. lima morphotype 1 (cf. Zhang et al, 2015) in the LSU tree (Fig. 6).…”

Section: Genetic Divergence and Molecular Phylogeny Of Prorocentrummentioning

confidence: 99%

Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae)

Lim

Luo²,

Lee

et al. 2019

Harmful Algae

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A B S T R A C TThirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a new morphotype, herein designated as P. malayense sp. nov. Prorocentrum malayense, a species closely related to P. leve, P. cf. foraminosum, P. sp. aff. foraminossum, and P. concavum (Clade A sensu Chomérat et al. 2018), is distinguished from its congeners as having larger thecal pore size and a more deeply excavated V-shaped periflagellar area. Platelet arrangement in the periflagellar area of P. malayense is unique, with the presence of platelet 1a and 1b, platelet 2 being the most anterior platelet, and a broad calabash-shaped platelet 3. The species exhibits consistent genetic sequence divergences for the nuclear-encoded large subunit ribosomal RNA gene (LSU rDNA) and the second internal transcribed spacer (ITS2). The phylogenetic inferences further confirmed that it represents an independent lineage, closely related to species in Clade A sensu Chomérat et al. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of compensatory base changes (CBCs). Toxicity analysis showed detectable levels of okadaic acid in P. lima (1.0-1.6 pg cell ˗1 ) and P. caipirignum (3.1 pg cell ˗1 ); this is the first report of toxigenic P. caipirignum in the Southeast Asian region. Other Prorocentrum species tested, including the new species, however, were below the detection limit.

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“…The D1-D3 LSU and SSU rDNA regions were amplified using the primers D1R and D3B (Scholin et al 1994;Nunn et al 1996) and SSU-F/SSU-R (Zhang et al 2012), respectively, whereas the ITS region was amplified using the ITSc/ITSd primers (Leaw et al 2001) or the universal primers of internal transcriber spacer [ITS1 (5 0 -TCCGTAGGT-GAACCTGCGG-3 0 ) and ITS4 (5 0 -TCCTCCGCTTATTGATATGC-3 0 )]. The amplification reactions for D1-D3 LSU and ITS regions were performed based on the procedure previously used by Zhang et al (2015). For SSU, the following cycling conditions were employed: 5 min at 94 C, followed by 35 cycles of 20 s at 94 C, 30 s at 54 C and 2 min at 72 C, with a final extension of 10 min at 72 C. Sequencing was conducted with the ABI 3730XL BigDye V3.1 Mix technique (Applied Biosystems, Foster City, CA, USA).…”

Section: Dna Sequencing and Phylogenetic Analysesmentioning

confidence: 99%

Morphology and molecular phylogeny of Ostreopsis cf. ovata and O. lenticularis (Dinophyceae) from Hainan Island, South China Sea

Hua

Lü

et al. 2017

Phycological Research

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SUMMARY The genus Ostreopsis contains benthic and potentially toxic species that are distributed in tropical and temperate regions. In this study, we present the first report of observations of Ostreopsis cf. ovata and O. lenticularis in Chinese waters. Isolates were characterized morphologically based on LM and SEM, and phylogenetic analyses were conducted by sequencing the LSU rDNA (D1–D3), ITS region (ITS1‐5.8S‐ITS2) and SSU rDNA. The morphology of O. cf. ovata Hainan strains was similar to the original description of O. ovata, despite differences in cell size. The morphology of O. lenticularis Hainan strains was similar to the original description, although both deviations of cell dimensions and different kinds of thecal pores were observed. The plate formula for the Ostreopsis Hainan strains was arranged in Kofoid's system tabulation of Po, 3′, 7′′, Vp, Rp, 1p, 5′′′, 2′′′′. The O. cf. ovata and O. lenticularis Hainan strains showed close relationships with strains collected from the Pacific Ocean based on the sequences of both LSU rDNA and ITS region. The genetic differences (p‐distances) of SSU rDNA sequences of the Ostreopsis cf. ovata Hainan strain were 0.0096 and 0.0447 different from those of a Malaysian strain and a South Korean strain, respectively. This work identifies the first occurrence of Ostreopsis with the morphology and phylogeny of Hainan strains in Chinese waters.

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Morphotypes ofProrocentrum lima(Dinophyceae) from Hainan Island, South China Sea: morphological and molecular characterization

Cited by 41 publications

References 47 publications

Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae)

Morphology and molecular phylogeny of Ostreopsis cf. ovata and O. lenticularis (Dinophyceae) from Hainan Island, South China Sea

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