Mortality Prevention in Septic Mice With Multiple Organ Dysfunction Syndrome: The Optimal Dose of Intraperitoneal Heat Shock Protein 70

Sukamto, Igl; Purwanto, Bambang; Mudigdo, Ambar; Suroto, Suroto; Murti, Bhisma; Ayuningrum, Ika Yuli

doi:10.22159/ajpcr.2018.v11i11.27773

Cited by 1 publication

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“…Sukamto et al (2018) conducted a research using LPS injection at a dose of 0.25 mg/kgBW intraperitoneally to create a sepsis model mouse. 4 Meanwhile, other study used larger LPS doses of up to 2 mg/kgBB, 5 mg/kgBB, and 10 mg/kgBB. 5 In this study, we will make a sepsis model animal seen from the requirements of inflammation and organ damage as evidenced by histopathological features of the kidneys and liver.…”

Section: Introductionmentioning

confidence: 99%

Degree of organ damage and inflammatory markers in sepsis mice models inducted by various doses of lipopolysaccharides 

Purwanto,

Indarto

et al. 2023

F1000Res

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Background Sepsis is a life-threatening disease, and animal models of sepsis are minimal. This study aims to find the optimal dose of LPS to make a sepsis mouse model by examining the presence of target organ damage. Methods This study used 30 mice divided into four groups. The control group (3 mice) injected 0.5 cc NaCl 0.9% intraperitoneally (i.p.). Group A (9 mice) was injected with lipopolysaccharides (LPS) 0.125 mg/kg B.W. i.p. given on the first and second day, group B (9 mice) was injected with LPS 0.15 mg/kg B.W. i.p. given on the first and second days, and group C (9 mice) was injected by LPS 0.3 mg/kg B.W. single dose i.p. On the third, fourth, and fifth days, the termination of each group of three mice and examination of the NF-κB, tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), alanine aminotransferase (ALT), the expression of NF-κB in the liver and kidneys, and histopathology in the liver and kidneys were conducted. Results NF-κB, C-reactive protein (CRP), alanine aminotransferase (ALT), NF-κB examinations, and tumor necrosis factor-α (TNF-α) in all treatment groups increased when compared with the control. The highest degree of histopathological features of the kidneys and liver and the results of immunohistochemistry examinations on the liver and kidneys were shown in group C. Conclusions The optimal dose of LPS to make a sepsis mouse model was 0.3 mg/kgB.W with the most severe target organ damage dan significant increased of inflammatory markers.

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Section: Introductionmentioning

confidence: 99%