Moslea Herba flavonoids alleviated influenza A virus-induced pulmonary endothelial barrier disruption via suppressing NOX4/NF-κB/MLCK pathway

Yu, Wen-Ying; Li, Lan; Wu, Fang; Zhang, Huan‐Huan; Fang, Jie; Zhong, Yu-Sen; Yu, Chunjing

doi:10.1016/j.jep.2020.112641

Cited by 29 publications

(28 citation statements)

References 43 publications

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“…Baicalein and quercetin were both flavonoids. Flavonoids reduced the barrier dysfunction induced by influenza A virus by inhibiting the NOX4/NF-κB/MLCK pathway, which might be a potential drug for the prevention and treatment of influenza A virus and pulmonary endothelial barrier dysfunction [ 35 ]. A study showed that baicalein inhibited the overactivation of the complement system in vivo and improved the acute lung injury induced by influenza a virus [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Huashi Baidu formula in the treatment of COVID-19

Tao

et al. 2020

Drug Development and Industrial Pharmacy

178

143

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Purpose: Huashi Baidu formula (HSBDF) was developed to treat the patients with severe COVID-19 in China. The purpose of this study was to explore its active compounds and demonstrate its mechanisms against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through network pharmacology and molecular docking. Methods: All the components of HSBDF were retrieved from the pharmacology database of TCM system. The genes corresponding to the targets were retrieved using UniProt and GeneCards database. The herb-compound-target network was constructed by Cytoscape. The target protein-protein interaction network was built using STRING database. The core targets of HSBDF were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The main active compounds of HSBDF were docked with SARS-CoV-2 and angiotensin converting enzyme II (ACE2). Results: Compound-target network mainly contained 178 compounds and 272 corresponding targets. Key targets contained MAPK3, MAPK8, TP53, CASP3, IL6, TNF, MAPK1, CCL2, PTGS2, etc. There were 522 GO items in GO enrichment analysis (p < .05) and 168 signaling pathways (p < .05) in KEGG, mainly including TNF signaling pathway, PI3K-Akt signaling pathway, NOD-like receptor signaling pathway, MAPK signaling pathway, and HIF-1 signaling pathway. The results of molecular docking showed that baicalein and quercetin were the top two compounds of HSBDF, which had high affinity with ACE2. Conclusion: Baicalein and quercetin in HSBDF may regulate multiple signaling pathways through ACE2, which might play a therapeutic role on COVID-19.

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Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Huashi Baidu formula in the treatment of COVID-19

Tao

et al. 2020

Drug Development and Industrial Pharmacy

178

143

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“…Kaempferol is avonoid found in FF, LIF, EH, FBR, HH, and LI of LHQW capsule, and can protect against in ammation, virus infection, endothelial barrier dysfunction, and cytokines release [35][36][37][38]. Another important compounds, such as luteolin, naringenin, and beta-sitosterol, in LHQW capsule also have these protective effects [39][40][41][42]. Thus, the results of GO analysis indicated that the mechanisms of LHQW capsule treating COVID-19 might be related with suppressing in ammation, virus infection, endothelial barrier dysfunction and cytokine storm.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Mechanisms of Lian Hua Qing Wen Capsule against COVID-19 by Network Pharmacology and Molecular Docking Approach

Chen¹,

Qu²,

Tan³

et al. 2020

Preprint

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Background The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or COVID-19) disease has led to a wide-spread global pandemic. There is no specific antiviral drug proven effective for the treatment of patients with COVID-19 at present. Combination of western and traditional Chinese medicine (TCM) is recommended, and Lian Hua Qing Wen (LHQW) capsule is a basic prescription and widely used to treat COVID-19 in China. However, the mechanisms of LHQW capsule treating COVID-19 are not clear. The aim of the study is to explore the mechanisms of LHQW capsule treating COVID-19 based on network pharmacy and molecular docking approach. Methods The active compounds and targets of LHQW capsule were obtained from traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). COVID-19 related target genes were obtained from GeneCards database and OMIM database. Protein–protein interaction (PPI) networks of LHQW capsule targets and COVID-19-related genes were visualized and merged to identify the candidate targets for LHQW capsule treating COVID-19. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were also performed. The hub genes involved in the gene-related pathways were screened and their corresponding compounds were used for in vitro validation of molecular docking predictions.Results A total of 185 active compounds of LHQW capsule were screened out, and 263 targets were predicted. Third hundred and fifty-two COVID-19 related target genes were obtained from GeneCards database and OMIM database. GO functional enrichment analysis showed that the biological processes of LHQW capsule treating COVID-19 were closely linked with the regulation of inflammation, immunity, cytokines production, vascular permeability, oxidative stress and apoptosis. KEGG enrichment analysis revealed that the pathways of LHQW capsule treating COVID-19 were significantly enriched in AGE−RAGE signaling pathway in diabetic complications, Kaposi sarcoma−associated herpesvirus infection, TNF, IL−17, and Toll−like receptor (TLR) signaling pathway. The hub targets genes in the gene-related pathways analysis of LHQW capsule treating COVID-19 included MAPK1, MAPK3, RELA, IL-6 and CASP8, which closely associated with inflammation, cytokines storm and apoptosis. Finally, molecular docking showed that top 5 compounds of LHQW capsule also had good binding activities to the important targets in COVID-19.Conclusions The mechanisms of LHQW capsule treating COVID-19 may involve in inhibiting inflammatory response, cytokine storm and virus infection, and regulating immune reactions, apoptosis and endothelial barrier.

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“…Natural products or traditional Chinese medicine have been reported to improve airway immunity, ameliorate the function of the blood-air barrier, inhibit inflammation, and reduce growth of pathogenic bacteria due to its advantages of multicomponent, multichannel, and multitarget ( Table 1 ), which had the potential for the prevention and treatment of several respiratory diseases including COPD, asthma, and UACS [ 63 ]. Baicalin, a kind of flavonoid derived from Scutellaria baicalensis (Huangqin in Chinese), with broad-spectrum antibacterial activity and obvious anti-inflammatory activity, which can effectively improve the respiratory system inflammation and treat respiratory disease by inhibiting a variety of bacteria and fungi [ 64 , 65 ]. Baicalin has an inhibitory effect on Candida albicans and Staphylococcus aureus , which was correlated with the concentration of baicalin, that is, the DNA synthesis of Candida albicans was decreased with the increase of drug concentration [ 66 , 67 ].…”

Section: Protective Effects Of Natural Products Against Various Stimuli-induced Lung Dysbiosismentioning

confidence: 99%

The Role of Respiratory Flora in the Pathogenesis of Chronic Respiratory Diseases

Guo

Chen

Ying

et al. 2021

BioMed Research International

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Large quantities of bacteria, including Firmicutes, Actinobacteria, and Bacteroidetes, colonize the surface of the respiratory mucosa of healthy people. They interact and coexist with the local mucosal immune system of the human airway, maintaining the immune stability and balance of the respiratory system. While suffering from chronic respiratory diseases, the microbial population in the airway changes and the proportion of Proteobacteria is increased in patients with asthma. The abundance of the microbial population in patients with chronic obstructive pulmonary disease (COPD) is decreased, and conversely, the proportion of Firmicutes and Proteobacteria increased. The diversity of airway microorganisms in cystic fibrosis (CF) patients is decreased, while pathogenic bacteria and conditional pathogenic bacteria are proliferated in large numbers. The proportion of Firmicutes and Proteobacteria is increased in patients with upper airway cough syndrome (UACS), which replaces the dominance of Streptococcus and Neisseria in the pharynx of a normal population. Therefore, a clear understanding of the immune process of the airway flora and the immune dysfunction of the flora on the pathogenesis of chronic respiratory diseases can provide new ideas for the prevention and treatment of human respiratory diseases.

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Moslea Herba flavonoids alleviated influenza A virus-induced pulmonary endothelial barrier disruption via suppressing NOX4/NF-κB/MLCK pathway

Cited by 29 publications

References 43 publications

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Huashi Baidu formula in the treatment of COVID-19

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Huashi Baidu formula in the treatment of COVID-19

Exploring the Mechanisms of Lian Hua Qing Wen Capsule against COVID-19 by Network Pharmacology and Molecular Docking Approach

The Role of Respiratory Flora in the Pathogenesis of Chronic Respiratory Diseases

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