Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication

Reyes-Ruiz, José Manuel; Osuna-Ramos, Juan Fidel; Bautista‐Carbajal, Patricia; Jaworski, Elizabeth; Soto-Acosta, Rubén; Cervantes-Salazar, Margot; Ángel-Ambrocio, Antonio H.; Castillo-Munguía, Juan Pablo; Chávez‐Munguía, Bibiana; Nova-Ocampo, Mónica De; Routh, Andrew; Ángel, Rosa M. del; Salas-Benito, Juan Santiago

doi:10.1016/j.virol.2019.02.018

Cited by 11 publications

(9 citation statements)

References 79 publications

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“…2019 ; Lee et al. 2019 ; Reyes-Ruiz et al. 2019 ) and (ii) transovarial transmission such that viromes are passed from infected females to their progeny ( Joshi, Mourya, and Sharma 2002 ; Lequime, Paul, and Lambrechts 2016 ; da Costa et al.…”

Section: Discussionmentioning

confidence: 99%

A time-series meta-transcriptomic analysis reveals the seasonal, host, and gender structure of mosquito viromes

et al. 2022

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Although metagenomic sequencing has revealed high numbers of viruses in mosquitoes sampled globally, our understanding of how their diversity and abundance varies in time and space, as well as by host species and gender, remains unclear. To address this we collected 23,109 mosquitoes over the course of 12 months from a bat-dwelling cave and a nearby village in Yunnan province, China. These samples were organized by mosquito species, mosquito gender and sampling time for meta-transcriptomic sequencing. A total of 162 eukaryotic virus species were identified, of which 101 were novel, including representatives of 17 RNA virus multi-family supergroups and four species of DNA virus from the families Parvoviridae, Circoviridae and Nudiviridae. In addition, two known vector-borne viruses – Japanese encephalitis virus and Banna virus – were found. Analyses of the entire virome revealed strikingly different viral compositions and abundance levels in warmer compared to colder months, a strong host structure at the level of mosquito species, and no substantial differences between those viruses harbored by male and female mosquitoes. At the scale of individual viruses, some were found to be ubiquitous throughout the year and across four mosquito species, while most of the other viruses were season and/or host specific. Collectively, this study reveals the diversity, dynamics and evolution of the mosquito virome at a single location and sheds new lights on the ecology of these important vector animals.

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Section: Discussionmentioning

confidence: 99%

A time-series meta-transcriptomic analysis reveals the seasonal, host, and gender structure of mosquito viromes

et al. 2022

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“…The salivary glands from SARS-CoV-2-infected patients have the ACE-2 and TMPRSS receptors overexpression (118). Also, the ultrastructural analysis of the ductal lining cell cytoplasm, acinar cells, and ductal lumen was performed by electron microscopy, a tool used to identify viral particles (119), revealed coronavirus-like particles (118). These findings demonstrated that the salivary glands are a reservoir for SARS-CoV-2, supporting the use of the saliva as a diagnostic method for COVID-19 and the role of this biological fluid in spreading the disease (118).…”

Section: Transmembrane Serine Proteases (Tmprss)mentioning

confidence: 99%

“…Cholesterol is an essential component of host cell membranes involved in tuning membrane fluidity, thickness, and permeability to regulate membrane function (172). The viral replication complexes are RNA virus-induced membrane structures where viral genome replication and morphogenesis occur (119). The formation of the replication complex requires cholesterol, a product of fatty acid metabolism (26,173).…”

Section: Targeting Cholesterol-rich Lipid Rafts As Potential Therapeutics In Sars-cov-2 Infectionmentioning

confidence: 99%

Cholesterol-Rich Lipid Rafts as Platforms for SARS-CoV-2 Entry

et al. 2021

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Since its appearance, the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2), the causal agent of Coronavirus Disease 2019 (COVID-19), represents a global problem for human health that involves the host lipid homeostasis. Regarding, lipid rafts are functional membrane microdomains with highly and tightly packed lipid molecules. These regions enriched in sphingolipids and cholesterol recruit and concentrate several receptors and molecules involved in pathogen recognition and cellular signaling. Cholesterol-rich lipid rafts have multiple functions for viral replication; however, their role in SARS-CoV-2 infection remains unclear. In this review, we discussed the novel evidence on the cholesterol-rich lipid rafts as a platform for SARS-CoV-2 entry, where receptors such as the angiotensin-converting enzyme-2 (ACE-2), heparan sulfate proteoglycans (HSPGs), human Toll-like receptors (TLRs), transmembrane serine proteases (TMPRSS), CD-147 and HDL-scavenger receptor B type 1 (SR-B1) are recruited for their interaction with the viral spike protein. FDA-approved drugs such as statins, metformin, hydroxychloroquine, and cyclodextrins (methyl-β-cyclodextrin) can disrupt cholesterol-rich lipid rafts to regulate key molecules in the immune signaling pathways triggered by SARS-CoV-2 infection. Taken together, better knowledge on cholesterol-rich lipid rafts in the SARS-CoV-2-host interactions will provide valuable insights into pathogenesis and the identification of novel therapeutic targets.

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“…The transmission of CHIKV to a mosquito vector can occur when a susceptible mosquito ingests blood from a viremic mammalian host. Key differences exist between mammalian-made and mosquito-made virions that affect infectivity (76)(77)(78)(79)(80)(81)(82). For example, differences in protein post-translation modifications between mammalian and invertebrate cells contribute to differences in virion infectivity (N-glycosylation (80,83)).…”

Section: Discussionmentioning

confidence: 99%

Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner

Miazgowicz

Ballista

Acciani

et al. 2022

Preprint

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Chikungunya virus (CHIKV), an alphavirus of the Togaviridae family, is the causative agent of the human disease chikungunya fever (CHIKF), which is characterized by debilitating acute and chronic arthralgia. No licensed vaccines or antivirals exist for CHIKV. Preventing the attachment of viral particles to host cells is an attractive intervention strategy. Viral entry of enveloped viruses from diverse families including Filoviridae and Flaviviridae is mediated or enhanced by phosphatidylserine receptors (PSRs). PSRs facilitate the attachment of enveloped viruses to cells by binding to exposed phosphatidylserine (PS) in the viral lipid membrane - a process termed viral apoptotic mimicry. To investigate the role of viral apoptotic mimicry during CHIKV infection, we produced viral particles with discrete amounts of exposed PS on the virion envelope by exploiting the cellular distribution of phospholipids at the plasma membrane. We found that CHIKV particles containing high outer leaflet PS (produced in cells lacking flippase activity) were more infectious in Vero cells than particles containing low levels of outer leaflet PS (produced in cells lacking scramblase activity). However, the same viral particles were similarly infectious in NIH3T3 and HAP1 cells, suggesting PS levels can influence infectivity only in cells with high levels of PSRs. Interestingly, PS-dependent CHIKV entry was observed in mosquito Aag2 cells, but not C6/36 cells. These data demonstrate that CHIKV entry via viral apoptotic mimicry is cell-type dependent. Furthermore, viral apoptotic mimicry has a mechanistic basis to influence viral dynamics in vivo in both the human and mosquito host.

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Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication

Cited by 11 publications

References 79 publications

A time-series meta-transcriptomic analysis reveals the seasonal, host, and gender structure of mosquito viromes

A time-series meta-transcriptomic analysis reveals the seasonal, host, and gender structure of mosquito viromes

Cholesterol-Rich Lipid Rafts as Platforms for SARS-CoV-2 Entry

Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner

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