Mosquito®: An Accurate Nanoliter Dispensing Technology

Jenkins, Joby; Cook, Matthew

doi:10.1016/j.jala.2004.04.020

Cited by 10 publications

(7 citation statements)

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“…To address this need, an impressive array of automated liquid-handling systems capable of nanoliter dispensing have emerged in recent years. [9][10][11][12] Some systems, such as the Beckman Coulter Echo series acoustic dispensers, provide high throughput and eliminate the possibility of cross-contamination through contactless dispensing. 13,14 However, these commercial systems often cost tens of thousands of dollars to >$100,000, putting them out of reach of many laboratories.…”

Section: Introductionmentioning

confidence: 99%

Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling

et al. 2021

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“…Thus, the best CV value for dispensing 100 nL was 0.7%, i.e. , 0.7 nL, which was significantly less than that observed for the current precision dispensers [11] , [17] . Based on these results, the following experiment was conducted with hydrophobic treatment.…”

Section: Resultsmentioning

confidence: 58%

“…Contact dispensing is useful for nanoliter and microliter volumes because it is a simple and reliable method of touching the wall of a container for dispensing liquid. One of the most stable techniques for dispensing is to use a positive-displacement-type pipette with a plastic tube and steel core plunger, which works as a piston without an air gap [11] . This method of using a disposable tip allows for a relatively precise dispensing with zero cross-contamination and almost-zero dead volume even if the solution has viscosity.…”

Section: Introductionmentioning

confidence: 99%

Precise Dispensing Technology Using Electroformed Tubes for Micro-Volume Blood Diagnosis

Ando

Hirano

Ishige

et al. 2018

IEEE J. Transl. Eng. Health Med.

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Precise dispensing of droplets is a crucial step for acquiring reliable blood diagnostic results. When the source sample volume is limited, the need for precise dispensing of submicroliter and nanoliter quantities is especially important. In this paper, we developed a positive-displacement high-precision dispensing technique using a nickel electroformed tube with an inner diameter accuracy of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$5~\mu \text{m}$ \end{document} or less. When dispensing variation of 100 nL was evaluated by using a photometric method, the most stable coefficients of variation (CV) were observed for a tube thickness of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$50~\mu \text{m}$ \end{document} with hydrophobic treatment, where the average CV value was 1.3%, i.e., 1.3 nL. Furthermore, the glucose concentration of 100- and 200-nL animal-based control serum was colorimetrically measured using enzymatic reactions without drying and mixing reagents. The CV value was approximately 6.36% at 100 nL and 3.23% at 200 nL, suggesting that several biochemical panels can be precisely measured even from less than one drop of blood. This positive-displacement dispenser ensures zero contamination and almost-zero dead volume, thus it would be useful for multi-panel clinical blood testing.

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“…The disposable tips are supplied in stackable racks suitable for automated handling. A 3-position deck nanoliter dispenser 20 was integrated on the right-hand side of the system. Aspirate/dispense on the nanoliter dispenser also operates with positive displacement using disposable microsyringes (supplied in large spools).…”

Section: Representative Resultsmentioning

confidence: 99%

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Gorrec

Löwe

2018

JoVE

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When high quality crystals are obtained that diffract X-rays, the crystal structure may be solved at near atomic resolution. The conditions to crystallize proteins, DNAs, RNAs, and their complexes can however not be predicted. Employing a broad variety of conditions is a way to increase the yield of quality diffraction crystals. Two fully automated systems have been developed at the MRC Laboratory of Molecular Biology (Cambridge, England, MRC-LMB) that facilitate crystallization screening against 1,920 initial conditions by vapor diffusion in nanoliter droplets. Semi-automated protocols have also been developed to optimize conditions by changing the concentrations of reagents, the pH, or by introducing additives that potentially enhance properties of the resulting crystals. All the corresponding protocols will be described in detail and briefly discussed. Taken together, they enable convenient and highly efficient macromolecular crystallization in a multi-user facility, while giving the users control over key parameters of their experiments.

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Mosquito®: An Accurate Nanoliter Dispensing Technology

Cited by 10 publications

References 0 publications

Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling

Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling

Precise Dispensing Technology Using Electroformed Tubes for Micro-Volume Blood Diagnosis

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

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