1973
DOI: 10.1021/bi00726a013
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Mössbauer studies of cytochrome P-450cam

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Cited by 137 publications
(58 citation statements)
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“…4,[20][21][22][23][24][25][26][27][28] Spectroscopic methods that have been used to probe the electron distribution in the d-orbitals of ferro-and ferriheme systems include NMR, 29,30 EPR and Mössbauer. [31][32][33] In systems with a low spin Fe(III) center, EPR is able to probe the energy splitting of the d xy , d xz , d yz orbitals, 34,35 and can thus provide insight into the π donation of the heme center compared to that of axial ligands. [36][37][38] While significant insight can be obtained using EPR spectroscopy, the interpretation of the g values in terms of orbital energies is complicated due to the effects of covalency on the spin-orbital coupling.…”
Section: Introductionmentioning
confidence: 99%
“…4,[20][21][22][23][24][25][26][27][28] Spectroscopic methods that have been used to probe the electron distribution in the d-orbitals of ferro-and ferriheme systems include NMR, 29,30 EPR and Mössbauer. [31][32][33] In systems with a low spin Fe(III) center, EPR is able to probe the energy splitting of the d xy , d xz , d yz orbitals, 34,35 and can thus provide insight into the π donation of the heme center compared to that of axial ligands. [36][37][38] While significant insight can be obtained using EPR spectroscopy, the interpretation of the g values in terms of orbital energies is complicated due to the effects of covalency on the spin-orbital coupling.…”
Section: Introductionmentioning
confidence: 99%
“…Binding of substrate to P450s displaces a weakly ligated water ligand that occupies the sixth coordination site of the heme iron, hence the iron geometry changes from octahedral (low spin, S ϭ 1/2) to squarebased pyramidal (high spin, S ϭ 5/2) (8,11). Unfortunately, determination of the substrate dissociation constant for the reduced form, K d (red), cannot be measured in an analogous fashion to the oxidized form, as the substrate-free and substrate-bound ferrous complexes are both high spin and hence exhibit indistinguishable absorption spectra (A max ϭ 410 nm) (13,15). There are several techniques available that allow us to calculate ferrous P450 dissociation constants such as isothermal titration calorimetry and potentiometry (using ϪRTln(K-…”
mentioning
confidence: 99%
“…The advantages of this procedure are the rapid elution (4 rain) and the low dilution (nearly twice) of the substrate-free ferric cytochrome, both factors leading to its increased stability [7].…”
Section: Low Temperature Separationmentioning
confidence: 99%