Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles

Oliveira, J.B.A.; Petersen, Claudia G.; Massaro, Fabiana C.; Baruffi, R.L.R.; Mauri, A.L.; Silva, Liliane F. I.; Ricci, Juliana M.B.; Franco, J.G.

doi:10.1186/1477-7827-8-56

Cited by 37 publications

(34 citation statements)

References 26 publications

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“…The frequency of motile spermatozoa with large nuclear vacuoles ranged from 25.9 to 38.0%, and varied according to the definition of large vacuoles, the sample size, and the patient population [Oliveira et al 2010b;Franco et al 2012;Perdrix et al 2011;Perdrix et al 2012]. The present study found that the % LNV in motile spermatozoa was 28.0%, which was consistent with previous reports.…”

Section: Discussionsupporting

confidence: 82%

“…Therefore, there is a possibility that the large population of varicoceles introduce a bias to the results. Oliveira et al [2010b] reported that males with varicoceles presented more spermatozoa with LNV in their study using semen samples from 240 patients (OR = 1.02; 95% CI = 1.00 to 1.03). The semen parameters were not significantly different between those with varicoceles and those with idiopathic male infertility in our clinical practice [Komiya et al 2012].…”

Section: Discussionmentioning

confidence: 99%

“…The present study found that the % LNV in motile spermatozoa was 28.0%, which was consistent with previous reports. To [Boitrelle et al 2011], or 50% of the sperm head area [Franco et al 2008;Mauri et al 2010;Oliveira et al 2010a;2010b], or vacuoles with a diameter of more than 1.5 µm and visible at 400x magnification [Watanabe et al 2011], or vacuoles considered as large without any precise criterion [Garolla et al 2008]. Because the calculation of vacuole area and sperm nuclear area is time-consuming, we used a simplified definition according to that proposed by Boitrelle et al [2011] and Watanabe et al [2011], obtaining similar frequency of large nuclear vacuoles.…”

Section: Discussionmentioning

confidence: 99%

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Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Komiya

Watanabe

Kawauchi

et al. 2012

Systems Biology in Reproductive Medicine

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This study compared the sperm nuclear vacuoles and semen quality in the evaluation of male infertility. One hundred and forty-two semen samples were obtained from patients who visited the Male Infertility Clinic at Toyama University Hospital. Semen samples were evaluated by conventional semen analyses and the Sperm Motility Analysis System (SMAS). In addition, spermatozoa were analyzed at 3,700-6,150x magnification on an inverted microscope equipped with DIC/Nomarski differential interference contrast optics. A large nuclear vacuole (LNV) was defined as one or more vacuoles with the maximum diameter showing > 50% width of the sperm head. The percentage of spermatozoa with LNV (% LNV) was calculated for each sample. Correlations between the % LNV and parameters in SMAS and conventional semen analyses were analyzed. Processed motile spermatozoa from each sample were evaluated. The mean age of patients was 35 years old. Semen volume was 2.9 ± 1.6mL (0.1-11.0; mean ± standard deviation, minimum-maximum), sperm count was 39.3 ± 54.9 (x10 6 /mL, 0.01-262.0), sperm motility was 25.1 ± 17.8% (0-76.0), and normal sperm morphology was 10.3 ± 10.1% (0-49.0). After motile spermatozoa selection, we could evaluate % LNV in 125 ejaculates (88.0%) and at least one spermatozoon with LNV was observed in 118 ejaculates (94.4%). The percentage of spermatozoa with LNV was 28.0 ± 22.4% (0-100) and % LNV increased significantly when semen quality decreased. The correlation between the % LNV and the semen parameters was weak to moderate; correlation coefficients were -0.3577 in sperm count ( p < 0.0001), -0.2368 in sperm motility ( p = 0.0084), -0.2769 in motile sperm count ( p = 0.019), -0.2419 in total motile sperm count ( p = 0.0070), and -0.1676 in normal sperm morphology ( p = 0.0639). The % LNV did not show a significant correlation with the SMAS parameters except for weak correlation to beat/cross frequency (r = -0.2414, p = 0.0071). The percentage of spermatozoa with LNV did not have a strong correlation with parameters in conventional semen analysis and SMAS in the patients with male infertility; however, a certain level of negative influence of LNV to sperm quality cannot be excluded.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Komiya

Watanabe

Kawauchi

et al. 2012

Systems Biology in Reproductive Medicine

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“…However, a normal-looking sperm could still contain serious nuclear malformations and chromosomal aneuploidies (32). There are published reports that when intracytoplasmic morphologically selected sperm injection (IMSI) was performed with sperm selected under a magnification level above 6 000× (33), the rate of sperm nucleus normality was significantly higher.…”

Section: Discussionmentioning

confidence: 99%

The Effect of the Sperm Source on the Outcome of Intracytoplasmic Sperm Injection-Embryo Transfer Cycles in Normal Responder Women

Usta¹,

Erdem²,

Karacan³

et al. 2016

Acta Med Anatol

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Introduction:Poor semen quality is the main cause of male factor. The aim of this study was to evaluate the effect of sperm source on the outcome of intracytoplasmic sperm injection-embryo transfer cycles (ICSI-ET) in normoresponder women. Methods:A total of 884 normoresponder women, underwent first ICSI cycles were evaluated. Fertilization, cleavage, embryo morphology, clinical pregnancy, miscarriage and live birth rates were compared. Ejaculated semen samples were collected by masturbation. Sperm retrieval from the testis was performed percutaneously by microdissection testicular sperm extraction (m-TESE) under local anesthesia.Results: Patients were divided four group according to source of sperm and sperm parameters. Testicular spermatozoa obtained from men with azoospermia (group 1, n=43), severe oligoasthenoteratozoospermia (sOAT), (group 2, n=93), oligoasthenoteratozoospermia (OAT) (group 3, n=209) and normal semen analysis (group 4, n=539). The most reduced fertilization rate was observed with testicular spermatozoa and the fertilization rate increased as semen quality increased from Group 2 to 4. Our results indicated that semen quality can affect the fertilization process. We observed that the cleavage and high-quality embryo rates were highest in group I (88.4% and 93%, respectively) compared to other groups. Clinical outcomes of ICSI did not show statistically significant differences in the rates of clinical pregnancy, miscarriage and live birth rate. Conclusion:Neither sperm parameters nor the source of spermatozoa affects live birth rate in normoresponder women when motile/morphologically normal spermatozoa is present.Keywords: Azoospermia, Oligo-astheno-teratozoospermia, Semen quality, Intracytoplasmic sperm injection. Received: 17.03.2016 Accepted: 06.04.2016 doi: 10.5505/actamedica.2016 Abstract 56 Original ArticleUsta et al.

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“…At least 200 motile spermatozoa per semen sample were evaluated and percentage of abnormal spermatozoa was determined [16] . Fig.…”

Section: Determination Of Morphology By Msomementioning

confidence: 99%

Motil Sperm Organel Morfolojisi Muayenesi (MSOME) ve Fiksatif Solüsyon, İki Farklı Boyama Tekniği Ile Köpek Epididimal Spermatozoa Morfolojisinin Değerlendirilmesi

Cebisen¹,

Faúndez²,

Jurka³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to compare the use and effectiveness of two different stains, one fixative solution and Motile Sperm Organelle Morphology Examination (MSOME) on morphological characteristics of spermatozoa in fresh dog semen samples from epididymis. After routine castration, cauda epididymides were collected from 20 dog testes. Morphological abnormalities were evaluated by using Hancock's buffered formol saline solution, the aniline blue stain, Diff-Quik stain and MSOME analysis in epididymal semen. Conventional semen analysies and MSOME were simultaneously performed on the same sample from each dog. Percentage of abnormal spermatozoa, head abnormality and acrosomal defects were significantly higher in samples with two different staining methods and one fixative solution than MSOME technique. It was concluded that the usage of the Aniline blue stain may be an efficient method for evaluating the sperm morphology of dog semen. Although no correlation was established amoung four different methods on sperm morphology assessment. Keywords: Canine, Morphology, MSOME, Semen, Stain Motil Sperm Organel Morfolojisi Muayenesi (MSOME) ve Fiksatif Solüsyon, İki Farklı Boyama Tekniği İle Köpek Epididimal Spermatozoa Morfolojisinin Değerlendirilmesi ÖzetBu çalışmanın amacı, taze köpek sperması örneklerinde spermatozoonların morfolojik özellikleri üzerine Motil Sperm Organel Morfolojisi Muayenesi (MSOME), bir fiksatif solüsyon ve iki farklı boyanın kullanımını ve etkinliğini karşılaştırmaktı. Kastrasyon işleminden sonra, 20 adet köpek testisinden kauda epididimisler elde edildi. Epididimal spermada morfolojik bozukluklar, Hancock sıvı fikzasyon yöntemi, aniline blue boyama yöntemi, Diff Quik boyama yöntemi ve MSOME tekniği ile değerlendirildi. Her bir köpekten bir örnek alınarak, konvensiyonel semen analizi ve MSOME analizi aynı örnekte eş zamanlı olarak incelendi. Morfolojik olarak ortalama abnormal baş ve akrozom defekti MSOME tekniğine göre diğer üç farklı methodta önemli derecede yüksek bulunmuştur. MSOME tekniği ile 3 farklı yöntem karşılaştırıldığında, anilin boyama yönteminde baş bozukluk oranı bulunan anormal spermatozoa oranı önemli derecede yüksek bulunurken, köpek spermatozoa morfolojisi değerlendirilmesinde Aniline blue boyama yönteminin etkili bir yöntem olduğu sonucuna varıldı. Spermatozoa morfolojisinin değerlendirilmesinde üç farklı method ile MSOME tekniği arasında herhangi bir paralellik bulunmadı.

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Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles

Cited by 37 publications

References 26 publications

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

The Effect of the Sperm Source on the Outcome of Intracytoplasmic Sperm Injection-Embryo Transfer Cycles in Normal Responder Women

Motil Sperm Organel Morfolojisi Muayenesi (MSOME) ve Fiksatif Solüsyon, İki Farklı Boyama Tekniği Ile Köpek Epididimal Spermatozoa Morfolojisinin Değerlendirilmesi

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