Motility and biofilm production involved in the interaction of phosphate solubilizing endophytic strains with peanut, maize and soybean plants

Abstract

Endophytic plant growth promoting bacteria are of great interest since the ability to colonize the internal tissues of plants gives it an adaptive advantage. To confer any beneficial effect on the plant, bacteria must present a successful colonization and thus be competent in the rhizosphere and finally infect internal tissues. In this sense, bacterial motility is an essential property involved in plant-microorganism interaction where bacteria can colonize the root and at the same time form biofilms. Within plant beneficial bacteria those that present phosphate solubilizing activity are of great interest due to the contribution of phosphorus to plants. The objective of this work was to evaluate the motility and biofilm formation properties of the strains Serratia sp. S119 and Enterobacter sp. J49 growing in the presence of peanut, maize and soybean root exudates. Results obtained indicated that both strains have flagella, possess swimming, swarming and twitching motilities and showed differential chemotactic attraction against root exudates. In addition, the strains under study showed the ability to form biofilms, being this ability greater in minimal media, in which a greater decrease was also seen by the addition of root exudates in the first hours of growth. The changes produced by the addition of root exudates in the chemotaxis and biofilm formation of the strains suggests that compounds released by the plants are detected by these bacteria and could be part of the molecular dialogue involved in their interaction with the roots of plant.

Root systems of peanut cultivars respond differently to soil P availability to improve P uptake

“…Regardless of the cultivar, peanut cultivation reduced all fractions of soil P (Table 4). This corroborates the reported ability of peanuts to adapt to low-fertility sandy soils and use less labile P fractions (Kraimat & Bissati, 2017;Lucero et al, 2020;Shibata & Yano, 2003;Wissuwa & Ae, 2001;Zhu et al, 2022).…”

“…Additionally, the soil used for the pot experiment received limestone before the study started, which may help to explain this. Peanut has a high capacity to exude organic acids (Lucero et al., 2020; Xu et al., 2021), which may also be a strategy to acquire non‐labile P (Ludueña et al., 2017; Tang et al., 2020). However, the ability to exude organic acids may be different depending on the cultivar.…”

“…Peanut ( Arachis hypogaea L.) has been reported as a P‐efficient crop (Shibata & Yano, 2003; Wissuwa & Ae, 2001), which can acquire P from low‐labile soil pools (Ludueña et al., 2017; Tang et al., 2020). Peanut acquires this low‐labile P by (1) exuding organic acids (Janegitz et al., 2017), (2) altering root morphology to increase root hair, root system length (Wissuwa & Ae, 2001), and root surface area (Zhu et al., 2022), (3) increasing acid phosphatase activity in the rhizosphere (Inal et al., 2007), and (4) enhancing associations with bacteria and fungi (Lucero et al., 2020), thereby improving microbial activity in the rhizosphere (Chen et al., 2018; Zhang et al., 2021). However, previous studies of P acquisition by peanut have focused on erect cultivars from the Valencia group, which have lower yields (approx.…”

Bioprospecting and Challenges of Plant Microbiome Research for Sustainable Agriculture, a Review on Soybean Endophytic Bacteria

“…The ability of endophytic bacteria to produce phosphatases also helps in the mineralization of organic phosphorus [ 137 ]. In vitro screening of phosphate-solubilizing endophytic bacteria has been investigated from soybean, sunflower, and rapeseed [ 138 – 140 ]. For example, Acinetobacter calcoaceticus , Ochrobactrum haematophilum , B. panacihum , Bacillus subtilis , B. australimaris , B. thuringiensis , B. zhangzhouensis , and Lysinibacillus pakistanensis have been isolated from leguminous crops [ 9 , 18 , 30 ].…”