Motility and fertility of equine spermatozoa extended in bovine serum albumin and sucrose

Klem, Michelle; Kreider, J.L.; Pruitt, Jaclyn; Potter, G.D.

doi:10.1016/0093-691x(86)90163-9

Theriogenology

1986

DOI: 10.1016/0093-691x(86)90163-9

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Motility and fertility of equine spermatozoa extended in bovine serum albumin and sucrose

Michelle Klem¹,

J.L. Kreider²,

Jaclyn Pruitt³

et al.

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Cited by 10 publications

(1 citation statement)

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“…It is shown that BSA as antioxidant protects the sperm of rabbit and men from free radicals (Alvarez & Storey, 1983;. BSA is known to eliminate free radicals generated by oxidative stress and protect membrane integrity of equine and human sperm cells from heat shock during freeze-thaw process of semen (Kreider et al, 1985;Klem et al, 1986;Lewis et al, 1997). To our knowledge, the effect of BSA on oxidative characters including antioxidant enzyme activities, total antioxidant capacitaty (TAC), total thiol (TT) and LPO in cryopreserved bull spermatozoa has not previously been assessed.…”

mentioning

confidence: 99%

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Ashrafi

Kohram

Tayefi‐Nasrabadi

2013

Span. j. agric. res.

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The aim of this study was to determine the effects of bovine serum albumin (BSA) as an antioxidant on post-thaw characters of bull spermatozoa such as motion variables, viability, plasma membrane integrity, morphology, lipid peroxidation, total antioxidant capacity (TAC), total thiols (TT) and the enzymes activities. Ejaculates were collected from six proven bulls and diluted with a citrate-based extender supplemented with various concentrations of BSA (0, 0.5, 1, 1.5 and 2 g/100 mL). The results showed that the semen extender supplemented with various concentrations of BSA increased (p0.05) were detected in progressive motility, sperm track straightness, beat cross frequency, curvilinear velocity and malondialdehyde production between the experimental groups. The highest concentration of BSA (2 g/100 mL) in the semen extender reduced (p<0.05) the sperm motion variables. The results indicated that the most effective concentration of BSA is 1 g/100 mL in the semen extender which is associated with an increase in the total motility, antioxidant enzymes activities, TT and TAC.

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mentioning

confidence: 99%

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Ashrafi

Kohram

Tayefi‐Nasrabadi

2013

Span. j. agric. res.

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Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa

2010

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Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa

et al. 2017

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Motility and fertility of equine spermatozoa extended in bovine serum albumin and sucrose

Cited by 10 publications

References 9 publications

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa

Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa

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