Motility and Viability of Spermatozoa of Belgian Blue Crossbreeds with the Addition of Tomato (Solanum lycopersicum) Extract in Egg Yolk Citrate Diluent

Abstract

Crosses between local cattle and pure Belgian Blue cattle often causes birth difficulty because the calf size is too large. In order to avoid this problem, crossbreeding is not conducted with pure Belgian Blue cattle, but with Belgian Blue crossbreeds. In order to support the crossbreeding program between local cattle and Belgian Blue Crossbreeds, it is necessary to study the semen quality of Belgian Blue crossbreeds. This study was aimed to determine the effect of adding tomato (Solanum lycopersicum) extract to egg yolk citrate diluent on the motility of liquid semen and the viability of spermatozoa in Belgian Blue Crossbreed cattle stored at 5 o C. Semen was collected from a 3-year-old Belgian Blue Crossbreed, using an artificial vagina. Semen examination was carried out at the Animal Physiology and Reproduction Laboratory, Universitas Gadjah Mada. The treatments in this study consisted of egg yolk citrate diluent (Control), egg yolk citrate diluent and 4% of tomato extract (P1), egg yolk citrate diluent and 6% of tomato extract (P2), and egg yolk citrate diluent and 8% of tomato extract (P3). Data obtained from microscopic examination were analyzed using analysis of variance based on Completely Randomized Design (CRD). The results showed that the addition of tomato extract could improve maintaining (P<0.05) motility and viability of spermatozoa stored at 5 o C for 96 hours. Semen motility at P0, P1, P2, and P3 were 36.8%, 42.5%, 41.3% and 46.8%, respectively. The viability of spermatozoa at P0, P1, P2, and P3 was 61.3%, 68.5%, 69.3% and 74.3%, respectively. The addition of 8% of tomato extract presented the best results in maintaining motility (46.8%) and viability of spermatozoa (74.3%) after 96 hours. The conclusion of this study was that the addition of 8% of tomato extract into egg yolk citrate diluent could improve the quality of liquid semen, which included motility and viability of spermatozoa.

The Potential Protective Effect for Some Nutritional Sources of Lycopene on Prostatic Hypertrophy in Rats

“…In our investigation, administration of lycopene, sweet red pepper, and papaya fruits improved sperm count and sperm motility. This is in agreement with the findings by Bintara et al (37) who discovered that adding tomato extract to an egg yolk citrate diluent might enhance the spermatozoa's motility and viability, among other qualities of liquid semen. By increasing the antioxidant capacity of sperm, lycopene and lycopene-rich foods like papaya and red pepper fruits are thought to improve male infertility.…”

Effect of Green Tea Extract on Spermatozoa Quality of Peranakan Ongole Bull On Frozen Storage

“…Antioxidants were added to extenders can protect spermatozoa from oxidative stress caused by ROS during frozen storage (Allai et al, 2018). Giving an enormous amount of antioxidants can have a terrible impact because it leads to a loss of its effectiveness as an antioxidant and even causes peroxides (Wahjuningsih et al, 2021 ) This research was accomplished by adding Giyanita Rahma Ayu Pramesti, Nur Ducha / Biosaintifika 15 (2) (2023): 237-245 eosin negrosin to the semen that had been placed on an object-glass and making smear preparations (Bintara et al, 2022). This method is used to analyze the viability and evaluate the morphological structure of spermatozoa (Kondracki et al, 2017).Observations were done by color changes that appear on the spermatozoa if the coloring agent can penetrate the spermatozoa and turn them into dark pink color, and if the dye is not able to penetrate the spermatozoa so that the spermatozoa are not stained, the spermatozoa are still alive (Fischer et al, 2020).…”