ABSTRAKPenelitian ini bertujuan mengetahui polimorfisme gen leptin sapi PO Kebumen dan asosiasinya dengan sifat pertumbuhan. Seratus sampel darah sapi dikoleksi untuk analisis molekuler. Polimorfisme gen leptin dianalisis menggunakan 2 teknik: Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi HpyCH4V dan sekuensing. Asosiasi antara gen leptin dan sifat pertumbuhan dianalisis menggunakan T-test. Hasil penelitian menunjukkan ada SNP g. 1180 C>T pada gen leptin populasi Sapi PO Kebumen, yang merubah asam amino dari arginin menjadi sistein. Dua alel, C dan T, dengan frekuensi masing-masing 0,885 dan 0,115 dan 3 genotip, CC, CT dan TT, dengan frekuensi masing-masing 0,78; 0,21 dan 0,01 telah dideteksi. Populasi yang diamati berada dalam Hardy-Weinberg equilibrium. Terdapat asosiasi yang signifikan antara genotip dan lingkar dada saat sapih. Dapat disimpulkan bahwa gen leptin merupakan kandidat gen yang dapat digunakan sebagai marker seleksi untuk lingkar dada saat sapih pada sapi PO Kebumen. ABSTRACTThe aim of this study was to identify the polymorphism of leptin gene and its association with growth traits in Kebumen Ongole Grade cattle. One hundred blood samples were collected for molecular analysis. Polymorphism of the leptin gene was analyzed using Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) with HpyCH4V restriction enzyme and DNA sequencing. Association analysis of the leptin gene with growth traits was analyzed by T-test. The results showed that SNP g. 1180 C>T was found in the population. The SNP changed amino acid from arginine to cysteine. The SNP was significantly associated with a high chest circumference at weaning age in animal having CC genotype (P<0.05). There were two identified alleles, namely C and T, with frequencies were 0.885 and 0.115, respectively. The genotype frequencies of CC, CT and TT were 0.78, 0.21 and 0.01, respectively. Allelic and genotypic distribution in the studied population were in Hardy-Weinberg equilibrium. Animals with CC genotype had a higher circumference at weaning age (WCC) than those with CT genotype. In conclusion, SNP g. 1180 C> T in the leptin gene is potential as genetic marker for growth traits in Kebumen Ongole Grade cattle.

INTISARITujuan dari penelitian ini adalah untuk mengetahui kinerja reproduksi dari sapi betina Sumba Ongole (SO) yang diinseminasi buatan (IB) dengan semen beku Belgian Blue (BB). Penelitian ini diharapkan dapat digunakan sebagai acuan dalam pemeliharaan sapi betina khususnya dalam pelaksanaan IB, serta hasil penelitian ini juga dapat digunakan sebagai bahan untuk rujukan atau acuan penelitian-penelitian selanjutnya. Parameter penelitian meliputi tanda-tanda gejala berahi, service per conception ratio (S/C ratio), conception rate (CR), non return rate (NRR), dan lama bunting. Hasil penelitian menunjukan bahwa besar nilai S/C untuk sapi SO 2,60±1,81, besar nilai CR untuk sapi SO yaitu 40%, nilai NRR sebesar 60%, dan lama bunting sapi SO 273,80±4,08. Berdasarkan penelitian yang dilakukan dapat disimpulkan bahwa kinerja sapi betina SO bagus sehingga penggunaan sapi betina SO sebagai indukan untuk disilangkan dengan sapi BB sangat mungkin untuk dilakukan.(Kata kunci: Belgian Blue, Kinerja reproduksi, Sumba Ongole) ABSTRACT PendahuluanSapi Sumba Ongole (SO) merupakan salah satu bangsa sapi yang memiliki potensi yang sangat baik untuk dikembangkan sebagai sapi potong, bila dibandingkan dengan bangsa sapi potong lokal lain di Indonesia karena umur dewasa kelamin 9 -18 bulan, lama berahi 20 -30 jam, siklus berahi 18-26 hari, umur beranak pertama 3,1 -3,2 tahun, dan jarak beranak 17 -19 bulan (Sodiq dan Hidayat, 2014). Di sisi lain, usaha pengembangan sapi potong menggunakan pengembangan sapi potong menggunakan sapi SO masih dirasa cukup lambat dan kurang, sehingga mengakibatkan jumlah populasi ternak sapi lokal yang ada belum dapat memenuhi kebutuhan konsumsi dan kebutuhan daging di Indonesia. Hal ini menyebabkan pemerintah melakukan upaya pemenuhan kebutuhan daging sapi melalui impor. Selain itu, peningkatan taraf perekonomian masyarakat sebesar 6% per tahun dan pertambahan jumlah penduduk sebesar 1,17% per tahun telah menyebabkan kebutuhan konsumsi daging

This research was conducted to identify the estrogen hormone profile and estrus response in the thin tailed ewes synchronized with controlled internal drug release (CIDR) implant. This research was carried out by employing 8 thin tailed ewes, CIDR, and estrogen kit. The ewes were distributed into: 3 ewes as control group and 5 ewes as treated group (with 12 days-CIDR implantation). Ewes were raised in the Sidomukti farm group, Sleman Regency, Yogyakarta. The data obtained were estrogen hormone profile during estrus examined by using ELISA method and estrus response marked with the reddening and oedema of vulva, mucus vaginal discharge, behavioral changes, vaginal pH, and superficial cells population. The data were analyzed by applying independent sample T-test. The result did not show any significant difference (P0.05) in the normal range between the control and CIDR group in estrogen hormone profile, estrus response, and pH level, but showed significant differences with positive correlation (P0.05) in population of superficial cells. In conclusion, CIDR was an effective tool for estrus synchronization at the farmer group, and gave positive estrus response towards thin tailed ewes with estrogen hormone profile, pH level, and superficial cell percentage on the normal range.

The purpose of this study was to observe the effect of extender and cooling rate on the quality of frozen thawed semen of Bali bull (Bos sondaicus). The experiment was conducted at Laboratory of Animal Biotechnology, Faculty of Animal Husbandry, Andalas University. A completely randomized design of factorial 2 x 3, 2 different extenders and 3 cooling rates with 3 bulls as the replicate. Data were analyzed using analysis of variance (ANOVA). The first factors was extender such as tris citrate egg yolk (TCEY) and tris citrate soy milk (TCSM). The second factors was the cooling rate such as: 15ºC/min, 10ºC/min and 5ºC/min. Variables were: motility, viability, abnormality, and membrane integrity. The results of treatment of TCEY extender on the average of motility 42.67±5.17%, viability of 53.78±3.79%, abnormality of 18.89±1.07%, and membrane integrity of 35.44±3.01%. The effect of cooling rate of 10°C /min has the highest semen motility of 44.35±5.28%, viability of 57.17±1.18%, abnormality of 17.84 ± 0.23 % and membrane integrity of 36.83±2.12%. The interaction between extender and cooling rate was founded significant different (P<0.05). Otherwise of extender and cooling rate on post-thawed of motility, viability and membrane integrity, had no difference (P>0.05) on semen abnormality. It can be concluded that tris-citrate egg yolk (TCEY) extender with cooling rate of 10ºC / min were the best semen motility, viability, and membrane integrity of spermatozoa.

The purpose of this study was to observed the potential use of coconut water + 20% egg yolk (CW) as an alternative diluent to replace Andromed®, on semen motility of Belgian Blue (BB) cattle. The sample were fresh semen collected from 3 years old BB using artificial vagina. The fresh semen was diluted into Andromed® diluent (P1) and coconut water + 20% egg yolk (P2). The semen motility (%) was observed at 0, 2, 4, 6, 12, 24 and 48 hours after dilution and then compared between group using t-test. The result showed that Andromed® found to give better motility compared to CW in all observation time. The average of semen motility (%) in accordance with observation time in group P1 was 77,67±2,51, 74,67±2,51, 72,33±2,51, 70,67±1,54, 69,33±1,54, 66,33±2,88 and 63,33±2,88%, respectively. Meanwhile, in P2 was 71,67±2,89, 65,00±5,00, 60,00±5,00, 58,33±2,89, 46,67±7,64, 41,67±7,64 and 26,67±7,64 % respectively. In conclusion, coconut water + 20% egg yolk could be replace the function of Andromed as diluent for AI using liquid semen with maximum storage time no more than 24 hours.