Polymorphism of the SNP g. 1180 C>T in leptin gene and its association with growth traits and linear body measurement in Kebumen Ongole Grade cattle

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui polimorfisme gen leptin sapi PO Kebumen dan asosiasinya dengan sifat pertumbuhan. Seratus sampel darah sapi dikoleksi untuk analisis molekuler. Polimorfisme gen leptin dianalisis menggunakan 2 teknik: Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi HpyCH4V dan sekuensing. Asosiasi antara gen leptin dan sifat pertumbuhan dianalisis menggunakan T-test. Hasil penelitian menunjukkan ada SNP g. 1180 C>T pada gen leptin populasi Sapi PO Kebumen, yang merubah asam amino dari arginin menjadi sistein. Dua alel, C dan T, dengan frekuensi masing-masing 0,885 dan 0,115 dan 3 genotip, CC, CT dan TT, dengan frekuensi masing-masing 0,78; 0,21 dan 0,01 telah dideteksi. Populasi yang diamati berada dalam Hardy-Weinberg equilibrium. Terdapat asosiasi yang signifikan antara genotip dan lingkar dada saat sapih. Dapat disimpulkan bahwa gen leptin merupakan kandidat gen yang dapat digunakan sebagai marker seleksi untuk lingkar dada saat sapih pada sapi PO Kebumen. ABSTRACTThe aim of this study was to identify the polymorphism of leptin gene and its association with growth traits in Kebumen Ongole Grade cattle. One hundred blood samples were collected for molecular analysis. Polymorphism of the leptin gene was analyzed using Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) with HpyCH4V restriction enzyme and DNA sequencing. Association analysis of the leptin gene with growth traits was analyzed by T-test. The results showed that SNP g. 1180 C>T was found in the population. The SNP changed amino acid from arginine to cysteine. The SNP was significantly associated with a high chest circumference at weaning age in animal having CC genotype (P<0.05). There were two identified alleles, namely C and T, with frequencies were 0.885 and 0.115, respectively. The genotype frequencies of CC, CT and TT were 0.78, 0.21 and 0.01, respectively. Allelic and genotypic distribution in the studied population were in Hardy-Weinberg equilibrium. Animals with CC genotype had a higher circumference at weaning age (WCC) than those with CT genotype. In conclusion, SNP g. 1180 C> T in the leptin gene is potential as genetic marker for growth traits in Kebumen Ongole Grade cattle.

Short Communication: Identification of Leptin gene in crossbred beef cattle

“…SNP g. 1180 C>Y in crossbred beef cattle was confirmed by electropherogram ( Figure 2). It has two alleles C and T. Hernández et al 2016; Orrù et al (2011);and Fathoni et al (2018) also reported that in Brahman; Simmental bulls; and Kebumen Ongole Grade cattle has SNP g. 1180 C>T (Arg/Cys). Fathoni et al (2018) reported a change in mutation g. 1180 C>T has significantly associated with high weaning chest circumference in Kebumen Ongole Grade cattle.…”

“…It has two alleles C and T. Hernández et al 2016; Orrù et al (2011);and Fathoni et al (2018) also reported that in Brahman; Simmental bulls; and Kebumen Ongole Grade cattle has SNP g. 1180 C>T (Arg/Cys). Fathoni et al (2018) reported a change in mutation g. 1180 C>T has significantly associated with high weaning chest circumference in Kebumen Ongole Grade cattle. Javanmard et al (2010); Buchanan et al (2002); Konfortov et al (1999) also reported SNP g. 1180 C>T change amino acid from arginine to cysteine.…”

“…The reactions were performed using a thermal cycle (PEQLAB Primus 25 advanced, Germany) with a pre-denaturation temperature at 94°C for 1 minute, followed by 30 cycles of reaction; denaturation at 94°C for 1 minute, annealing at a temperature of 57°C for 1 minute and extension at 72°C for 1 minute, then the last step was a final extension at 72°C for 5 minutes. The quality of the PCR product was determined using gel electrophoresis (1%), the thick and DNA bands were the preferred result (Fathoni et al, 2018). The targeted sequence was sequencing analyzed by LPPT UGM.…”

Polymorphism of leptin gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle

“…LEP hormone is encoded by the LEP gene. The bovine LEP gene is located in BTA 4q32 and consists of three exons and two introns [26]. The previous studies have associated SNP (c.73T>C) of LEP gene exon 2 with BW, fatness, and carcass quality [27,28].…”

The Potency of Leptin Gene as a Selection Marker of Economic Traits for Madura Cattle: Preliminary Study

“…Some researchers have associated polymorphism with phenotypic traits, although results vary. Fathoni et al [28] had stated that the Leptin gene polymorphism g.1080C>T was associated with weaning chest circumference in Kebumen Ongole Grade cattle. Meanwhile, other researchers stated no association between genotype and meat quality, birth weight, weaning weight, daily weight gain, and body condition score.…”