2017
DOI: 10.1017/s0967199416000435
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Motility, progressive motility score and DNA integrity of spermatozoa from cold-preserved mouse cauda epididymis

Abstract: This study attempted to investigate and validate whether epididymis cold storage could be a suitable alternative for short-term preservation of spermatozoa. Mouse cauda epididymides and spermatozoa were preserved at 4-8°C from 1 day to 6 weeks. From days 1 to 10, motility and fertility were daily examined when motility loss occurred. From week 1, spermatozoa were used for intracytoplasmic sperm injection (ICSI) at weekly intervals to test their fertility, and spermatozoa DNA integrity was determined by comet a… Show more

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