Abstract. 3D rotational coronary angiography plays an important role in the field of diagnosis and treatment planning of coronary artery disease. Due to the cardiac motion, only limited number of projections can be used to reconstruct coronary arteries for each heart phase, which makes the reconstruction problem ill-posed. To reduce the under-sampling artifacts, we apply an iterative method that makes use of total variation regularization. Some different reconstruction algorithms are compared and our method outperforms the others in the experiments.