Motion to Form a Quorum

Park, Sungsu; Wolanin, Peter M.; Yuzbashyan, Emil A.; Silberzan, Pascal; Stock, Jeffry B.; Austin, Robert H.

doi:10.1126/science.1079805

Cited by 134 publications

(113 citation statements)

References 8 publications

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“…Salman et al (2) observed swimming cells confined to narrow PDMS channels in linear thermal gradients and found that after 45 min, the cell concentration would peak at 34°C. At later times the peak would drift to lower temperatures, and more importantly, the velocity of the drift was proportional to the total initial cell density, implicating cell-cell interactions either via secreted signals (12) or by consumption of nutrients creating chemical gradients, as observed in soft-agar swarm plates. Another method is to measure the swimming behavior of cells in response to a temporal change in temperature.…”

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confidence: 99%

The thermal impulse response of Escherichia coli

Paster¹,

Ryu²

2008

Proc. Natl. Acad. Sci. U.S.A.

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Swimming Escherichia coli responds to changes in temperature by modifying its motor behavior. Previous studies using populations of cells have shown that E. coli accumulate in spatial thermal gradients, but these experiments did not cleanly separate thermal responses from chemotactic responses. Here we have isolated the thermal response by studying the behavior of single, tethered cells. The motor output of cells grown at 33°C was measured at constant temperature, from 10°to 40°C, and in response to small, impulsive increases in temperature, from 23°to 43°C. The thermal impulse response at temperatures < 31°C is similar to the chemotactic impulse response: Both follow a similar time course, share the same directionality, and show biphasic characteristics. At temperatures > 31°C, some cells show an inverted response, switching from warm-to cold-seeking behavior. The fraction of inverted responses increases nonlinearly with temperature, switching steeply at the preferred temperature of 37°C.thermotaxis ͉ taxis ͉ strategy

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confidence: 99%

The thermal impulse response of Escherichia coli

Paster¹,

Ryu²

2008

Proc. Natl. Acad. Sci. U.S.A.

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“…Because of their rapid generation times and the simplicity of their manipulation, microbial systems have been used to study a wide range of ecological processes [192][193][194][195][196]. On the other hand, the scale of microfluidic devices is ideal for application to microbial ecology, and a range of microbial processes has been successfully studied with microfluidics, including quorum sensing [197], chemotaxis [14,70,89], collective dynamics [198], and motility in confined environments [121,199]. Also, spatially structured microfluidic landscapes have recently been applied to the study of metapopulations of single [200] and competing bacterial species [201], and to demonstrate that microscale spatial structure enables coexistence [202].…”

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confidence: 99%

Microfluidics for bacterial chemotaxis

2010

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“…In the past, quorum-sensing was considered primarily in terms of bulk cell growth (11,12). However, cell-cell communication and chemotaxis might be a much more effective strategy for bacteria to actively form a quorum within a small cavity, as we have recently shown (13).…”

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confidence: 99%

Influence of topology on bacterial social interaction

Park

Wolanin

Yuzbashyan

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The environmental topology of complex structures is used by Escherichia coli to create traveling waves of high cell density, a prelude to quorum sensing. When cells are grown to a moderate density within a confining microenvironment, these traveling waves of cell density allow the cells to find and collapse into confining topologies, which are unstable to population fluctuations above a critical threshold. This was first observed in mazes designed to mimic complex environments, then more clearly in a simpler geometry consisting of a large open area surrounding a square (250 ؋ 250 m) with a narrow opening of 10 -30 m. Our results thus show that under nutrientdeprived conditions bacteria search out each other in a collective manner and that the bacteria can dynamically confine themselves to highly enclosed spaces.

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Motion to Form a Quorum

Cited by 134 publications

References 8 publications

The thermal impulse response of Escherichia coli

The thermal impulse response of Escherichia coli

Microfluidics for bacterial chemotaxis

Influence of topology on bacterial social interaction

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