Mould Spore Suspensions and Powders for use in Fungicidal Kinetic Studies: Part II. Preparations usingPenicillium spinuloswn

Abstract

Spore suspensions of Penicillium spinuloswn have been prepared containing over 99.0 per cent single spores in an even distribution. About 80 per cent of the spores were able both to produce germ tubes and to form colonies in a roll‐tube. A peptone powder containing an even distribution of spores has been prepared. The viable count of the powder did not show a significant fall during 5 months storage. The suspension has been successfully used to study the sporicidal and fungistatic activities of chlorocresol solutions.

The use of the Coulter Counter to detect the inactivation of preservatives by a non-ionic surface-active agent

“…Spore suspensions were prepared from 21 day cultures by the method of Gerrard, Harkiss & Bullock (1960). Bacillus subtilis NCTC 3610.…”

The Fungistatic Activity of Methyl and Propyl Hydroxybenzoates and A Mixture of These Against Penicillium Spinulosum

“…Penicillium spinulosum was chosen as a test organism since its suitability for such purposes has been established (Gerrard, Harkiss and Bullock, 1960). EXPERIMENTAL The test organism.…”

“…Suspensions of single spores in sterile water were prepared from 21 day old cultures grown on malt agar slopes by the method previously described by Gerrard, Harkiss and Bullock (1960). Double strength liquid maltose medium.…”