MOULTING CYCLE STAGES IN Macrobrachium rosenbergii BY SETOGENESIS METHOD

Abstract

This study had the objective of evaluating the duration of the moulting cycle stages of Macrobrachium rosenbergii, using the method of setogenesis. The experiment was conduced in the Prawn Farming Laboratory of the Federal University in Paraiba, in the town of Bananeiras, Paraiba, Brazil. Fifteen prawns (5.7 ± 0.3 g) were distributed in 3 aquariums (60 x 30 x 40 cm), maintained in natural photoperiod (12 x 12 hours), at a controlled temperature (28 °C), and feed was offered 3 times a day (8 a.m., 12 and 16 p.m.), throughout the experimental period. For identification, the animals were marked with colored silicone rings on the ocular peduncle and with colored plastic discs fixed on the carapace. From the setogenesis method, the prawns were classified into stage A, B, C, D0, D1, D2 or D3 of the moulting cycle. Setogenesis was observed stereomicroscopically every day, at the same time, 7 a.m., from the first ecdysis to the next ecdysis. The duration of the moulting cycle was 27.7 ± 3.2 days, and the intermoult stage (C) was the longest (8.0 ± 2.38 days). Stage A was the shortest (1 day), and the pre-moult stage (stages D) lasted 12.98+1.65 days. We concluded that juvenile of M. rosenbergii have a diecdysis type moulting cycle with shorter intermoult than pre-moult periods.

Molecular cloning and expression pattern of the juvenile hormone epoxide hydrolase gene from the giant freshwater prawn Macrobrachium rosenbergii during larval development and the moult cycle

“…To analyse tissue‐specific expression, tissues (eyestalk, heart, gill, stomach, haemocytes, hepatopancreas, muscle, testis, ovary, gut and thoracic ganglion) were dissected from five mature females and five mature males in inter‐moult stage. Additionally, the cephalothoraxes of healthy post‐larvae (PL 45) at different moult stages (inter‐moult, pre‐moult and post‐moult) were collected ( N = 5) after determination of the moult stages by setogenesis of the animals under a light microscope (da Silva et al, 2019).…”

La dureza total del agua afecta la muda, calcificación, crecimiento y supervivencia de Cryphiops caementarius (Palaemonidae)

“…El ciclo de muda comprende las etapas de postmuda, intermuda, premuda y culmina con la ecdisis (da Silva et al, 2019). En la postmuda hay digestión de gastrolitos para calcificación de la cutícula y el calcio restante requerido es tomado del agua por absorción branquial y por ingestión de la exuvia y de alimento; en cambio, en la premuda, el calcio del exoesqueleto es solubilizado desde la cutícula y transportado a las células, tejidos y órganos de almacenamiento temporal, y el exceso se excreta por las branquias (Wheatly, 1999;Ahearn et al, 2004).…”