We report airborne remote-sensing observations of a tropopause fold during two crossings of the polar 10 front jet over Northern Italy on 12 January 2016. The GLORIA (Gimballed Limb Observer for Radiance Imaging of the Atmosphere) observations allowed for a simultaneous mapping of temperature, water vapour and ozone.They revealed deep, dry and ozone rich intrusions into the troposphere. The mesoscale fine structures of dry filaments at the cyclonic shear side of the jet and tongues of moist air entraining tropospheric air into the stratosphere along the anticyclonic shear side were clearly resolved by GLORIA observations. Vertically 15 propagating mountain waves with recorded temperature residuals exceeding ± 3 K were detected above the Apennines. Their presence enhanced gradients of all variables locally in the vicinity of the tropopause. The combination of H2O-O3-correlations with potential temperature reveals an active mixing region and shows clear evidence of troposphere-to-stratosphere and stratosphere-to-troposphere exchange. High-resolution short-term deterministic forecasts of ECMWF's integrated forecast system (IFS) applying GLORIA's observational filter 20 reproduce location, shape, and depth of the tropopause fold very well. The fine structure of the mixing region, however, cannot be reproduced even with the used 9 km horizontal resolution of the IFS. This case study demonstrates convincingly the capabilities of linear limb-imaging observations to resolve mesoscale fine structures in the upper troposphere and lower stratosphere, validates the high quality of the IFS data, and suggests that mountain wave perturbations have the potential to modulate exchange processes in the vicinity of tropopause folds. 25Atmos. Chem. Phys. Discuss., https://doi.