Mouse granulocyte–macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions

Cui, Xiang‐Shun; Lee, Jae Yeong; Choi, Seok Hwa; Kwon, Mo Sun; Kim, Teoan; Kim, Nam‐Hyung

doi:10.1016/j.anireprosci.2003.11.005

Cited by 32 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the results obtained in this in vitro study showed that LIF and GM-CSF act as a growth factor or a proliferative factor causing an increase in the cell number of blastocysts obtained from treated groups than control. Although in agreement with this conclusion, a similar effect of LIF [20] and GM-CSF [14,29,30] on the cell number of intact pre implantation embryos was shown. Desai et al showed GM-CSF enhanced cell survival and prevented apoptosis of mouse embryo but did not influence overall cell number in developing blastocysts [31].…”

Section: Discussionsupporting

confidence: 83%

“…By several investigators an alternative procedure was introduced to improve the rate of in vitro embryo development to blastocysts stage using supplementation of medium with growth factors and cytokines including leukemia inhibitory factor (LIF) [8][9][10][11] and granulocytemacrophage colony stimulating factor (GM-CSF) [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that GM-CSF and LIF are embryo trophic in mammals and several studies have shown the supplementation of embryo culture media with LIF increase blastocyst hatching and the implantation rate and also decrease embryo degeneration [8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

Sheikholslami

Salehnia

Valojerdi

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Methods The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured. Results The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P<0.05). No 1/8 blastomeres developed to morula and blastocyst stages. Conclusions LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

Sheikholslami

Salehnia

Valojerdi

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…In previous research, GM-CSF inhibited the cellular stress response and apoptosis pathways to facilitate embryo growth and survival [10]. In bovine [13] and porcine [12,26] embryos, GM-CSF also increases the percentage of embryos that develop to the blastocyst stage in vitro.…”

mentioning

confidence: 99%

“…GM-CSF is expressed during early pregnancy in epithelial cells lining the oviduct and uterus in humans [9], rodents [37] and other mammalian species [15,32]. It enhances the development of preimplantation embryos in vitro and increases total cell numbers in the embryos of humans [41], mice [38], cows [13] and pigs [12,26]. Human embryos exposed to recombinant human GM-CSF in vitro blastulate earlier, have increased cell numbers in both the inner cell mass (ICM) and trophectoderm (TE) with reduced apoptosis [42] and show improved ability to hatch and attach to the culture dish [41].…”

mentioning

confidence: 99%

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Kwak

Cheong

Jeon

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNTderived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (P<0.05) increased blastocyst formation and total cell number in blastocysts compared with the control (12.3% and 41.4 vs. 9.0% and 34.7, respectively). However, there was no effect on cleavage rate. The numbers of cells in the inner cell mass and trophectoderm were significantly higher in the pGM-CSF treatment group (6.0 and 43.0, respectively) compared with the control (4.4 and 31.9, respectively). Treatment with 10 ng/ml pGM-CSF significantly increased POU5F1 and Cdx2 mRNA expression in blastocysts. In addition, Bcl-2, Dnmt1 and proliferating cell nuclear antigen (PCNA) mRNA expression were upregulated in blastocysts in the pGM-CSF supplemented group compared with the control. These results suggest that pGM-CSF improves the quality and developmental viability of porcine SCNT embryos by regulating transcription factor expression.

show abstract

Piglets produced from cloned blastocysts cultured in vitro with GM‐CSF

Lee

Redel

Spate

et al. 2013

Molecular Reproduction Devel

View full text Add to dashboard Cite

SUMMARY In general, pig embryos established by somatic cell nuclear transfer (SCNT) are transferred at the one-cell stage because of suboptimal embryo culture conditions. Improvements in embryo culture can increase the practical application of late embryo transfer. The goal of this study was to evaluate embryos cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro, and to track the in vivo developmental competency of SCNT-derived blastocysts from these GM-CSF embryos. The receptor for GM-CSF was up-regulated in in vitro-produced embryos when compared to in vivo-produced cohorts, but the level decreased when GM-CSF was present. In vitro fertilized (IVF) embryos, supplemented with GM-CSF (2 or 10 ng/ml), showed a higher frequency of development to the blastocyst stage compared to controls. The total cell numbers of the blastocysts also increased with supplementation of GM-CSF. Molecular analysis demonstrates that IVF-derived blastocysts cultured with GM-CSF exhibit less apoptotic activity. Similarly, an increase in development to the blastocyst stage and an increase in the average total-cell number in the blastocysts were observed when SCNT-derived embryos were cultured with either concentration of GM-CSF (2 or 10 ng/ml). When SCNT-derived embryos, cultured with 10 ng/ml GM-CSF, were transferred into six surrogates at Day 6, five of the surrogates became pregnant and delivered healthy piglets. Our findings suggest that supplementation of GM-CSF can provide better culture conditions for IVF- and SCNT-derived embryos, and pig SCNT-derived embryos cultured with GM-CSF in vitro can successfully produce piglets when transferred into surrogates at the blastocyst stage. Thus, it may be practical to begin performing SCNT-derived embryo transfer at the blastocyst stage.

show abstract

Mouse granulocyte–macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions

Cited by 32 publications

References 28 publications

Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Piglets produced from cloned blastocysts cultured in vitro with GM‐CSF

Contact Info

Product

Resources

About