Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Lin, Yi Wen; Hsu, T.-H.; Yen, Pauline H.

doi:10.1038/aja.2013.46

Cited by 9 publications

(12 citation statements)

References 34 publications

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“…heights are approximately 1.2μm smaller, consistent with our measurements lacking the 20 acrosomal cap (~0.15μm [30]), and the proteinaceous part of the sperm hook. We measured 21 the CBAs to be 12% longer than the C57Bl6s, again close to the previously published 22 13.5%.…”

supporting

confidence: 90%

A high-throughput method for unbiased quantitation and categorisation of nuclear morphology

Skinner

Rathje

Bacon

et al. 2018

Preprint

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2The physical arrangement of chromatin in the nucleus is cell type and species specific. This 3 is particularly evident in sperm, in which most of the cytoplasm has been lost; the shape of 4 the nucleus reflects the shape of the cell. Mice have distinctive falciform ('hook shaped') 5 sperm heads and nuclei. Quantification of the differences in shape variation between mouse 6 species and lines often relies on manual measurement and classification that leads to 7 subjective results, making comparisons within and between samples difficult. 8 9We have developed an analysis program for assessing the morphology of asymmetric 10 nuclei, and characterised the sperm of mice from a range of inbred, outbred and wild-derived 11 mouse lines. We find that laboratory lines have elevated sperm shape variability both within 12 and between samples in comparison to wild-derived inbred lines, and that sperm shape in 13 the F1 offspring of CBA and C57Bl6J lines is subtly affected by the direction of the cross. 14 15Hierarchical clustering can distinguish distinct sperm shapes with greater efficiency and 16 reproducibility than even experienced manual assessors. We quantified the range of 17 morphological defects in the inbred BALB/c line, demonstrating we can identify different 18 morphological subgroups. This approach has applications for studies of sperm development, 19 infertility and toxicology. 20

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supporting

confidence: 90%

A high-throughput method for unbiased quantitation and categorisation of nuclear morphology

Skinner

Rathje

Bacon

et al. 2018

Preprint

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“…Moreover, we show a β1 integrin presence ( Fig. S2,4 ) in a three point “bridge” like structure in the apical tip of the sperm hook, described as the hook rim 43 . This “bridge” is supporting the tip of the nucleus, anchoring it to the very tip of the hook.…”

Section: Discussionmentioning

confidence: 75%

“…The presence of CD46 was completely absent though. This structure was previously described as a hook rim 43 , but its protein content or function remains unclear.…”

Section: Resultsmentioning

confidence: 99%

“…This “bridge” is supporting the tip of the nucleus, anchoring it to the very tip of the hook. Supposedly, it has a resemblance of a sperm tail 43 , but this has not been further characterised with regards to a protein composition. We would expect there to be a membrane-surrounded structure based on the specific presence of β1 integrin, but due to a complete absence of CD46, it is unlikely to be formed by acrosome vesicle membranes.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction

Frolikova

Šebková

Děd

et al. 2016

Sci Rep

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The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It is widely recognized that specific sperm proteins form an active network prior to fertilization, and their dynamic relocation is crucial for the sperm-egg fusion. The unique presence of the membrane cofactor protein CD46 in the sperm acrosomal membrane was shown, however, its behaviour and connection with other sperm proteins has not been explored further. Using super resolution microscopy, we demonstrated a dynamic CD46 reorganisation over the sperm head during the AR, and its interaction with transmembrane protein integrins, which was confirmed by proximity ligation assay. Furthermore, we propose their joint involvement in actin network rearrangement. Moreover, CD46 and β1 integrins with subunit α3, but not α6, are localized into the apical acrosome and are expected to be involved in signal transduction pathways directing the acrosome stability and essential protein network rearrangements prior to gamete fusion.

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“…The tail segment is attached to the basal plate of the nucleus and to the nine fibers of the flagellum posteriorly. [8][9][10][11] . Artificial sweeteners are sweetening agents that include many compounds with different chemical structures and sizes.…”

Section: Introductionmentioning

confidence: 99%

Commercial Artificial Sweeteners Affect Spermatogenesis in Mice

Al-Qudsi

Alaa

2022

Int J Life Sci Pharm Res

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The use of artificial sweeteners has increased in recent years. More young people are using them as a noncaloric sweetener in coffee or tea. They are also present in many manufactured food products such as biscuits and cakes. Many studies explored the potential biological effects of artificial sweeteners. However, most studies explored single types of artificial sweeteners, while very few were done on the actual commercial artificial sweeteners compounds present in the market and used the normal permitted daily dose. Spermatogenesis is one of the important biological functions that leads to the production of the male germ cells, the sperms. Studies showed that male infertility has increased in recent years.-The aim of this research was to study the effect of commercial artificial sweeteners on mouse spermatogenesis. Male albino mice were divided in two main groups: 1.Control group and 2.Treated group. Each group was then divided into 4 groups (C1,C2,C3 and C4) and (T1,T2,T3 and T4). Treated Swiss albino male mice were orally given 50mg/kg commercial artificial sweetener solution for four weeks. While the controls were given distilled water. Artificial sweeteners caused multiple malformations in spermatozoa such as a ring shape at the end of the sperm tail, coiled sperm, short tail and cytoplasmic droplet seen in the mid-piece compared to the controls. Alteration of spermatogenesis process was seen in treated testis histological sections compared to the controls, by the increased number of deformed seminiferous tubules, the shrinkage in their size and the disappearance of the membrane surrounding some of the seminiferous tubules causing their fusion. Our results showed that daily consumption of 50mg/kg of commercial artificial sweeteners affects the spermatogenesis process in mice and produces malformed sperms.

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Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Cited by 9 publications

References 34 publications

A high-throughput method for unbiased quantitation and categorisation of nuclear morphology

A high-throughput method for unbiased quantitation and categorisation of nuclear morphology

Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction

Commercial Artificial Sweeteners Affect Spermatogenesis in Mice

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