Movement characteristics of human spermatozoa analysed from kinemicrographs

Duijn, C. Van; Voorst, C. van; Freund, M.

doi:10.1016/0028-2243(71)90051-7

Cited by 31 publications

(11 citation statements)

References 4 publications

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“…Visual methods of motility assessment cannot be used to determine sperm movement characteristics with accuracy or precision (van Duijn, Van Voorst & Freund, 1971 ; Katz & Dott, 1975 Experiments 3 and4. These were performed at 37 ± 0-5cC.…”

Section: Introductionmentioning

confidence: 99%

The movement of human spermatozoa in cervical mucus

Katz¹,

Mills²,

Pritchett³

1978

Reproduction

101

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Summary. Movement characteristics of freely swimming spermatozoa were studied with high-speed cinemicrography. At 21\ s=deg\ C, flagellar beat frequency was higher in midcycle human cervical mucus than in native semen or Tyrode's solution; the beat shape differed, possessing diminished amplitude and wavelength. Although the spermatozoa swam straighter in the mucus, the progressive swimming speeds did not differ in the three media. Swimming speed and beat frequency were linearly related in semen and in Tyrode, but in mucus the linearity was less certain. In midcycle cervical mucus at 37\s=deg\C, beat frequencies and swimming speeds were greater than at 21\ s=deg\ C, but the trajectories were equally straight, and the distances swum per beat (kinetic efficiencies) did not differ.

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Section: Introductionmentioning

confidence: 99%

The movement of human spermatozoa in cervical mucus

Katz¹,

Mills²,

Pritchett³

1978

Reproduction

101

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“…Walton, 1952;Rothschild, 1948Rothschild, , 1953Rikmenspoel, 1957;Harvey, 1945Harvey, , 1960Janick & Macleod, 1970;van Duijn, van Voorst & Freund, 1971).…”

Section: Introductionmentioning

confidence: 97%

The Estimation of the Proportion of Motile Bull Spermatozoa in Various Diluents and a Comparison With the Proportion Eosinophilic

Dott¹

1975

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The proportion of motile spermatozoa in bull semen diluted with phosphate-buffered saline, citrate or milk has been estimated both photographically and with an image-analysing computer. The two methods produced similar estimates. The proportion of noneosihophilic spermatozoa was higher than the proportion of immotile spermatozoa, particularly when fluoride or formaldehyde was added to the diluents. The proportion of non-eosinophilic spermatozoa in diluents containing formaldehyde was the same as in the original semen.

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“…Two reports on these data included a comparison of time-lapse photography measurements with visual ratings [23] and a comparison of a frame-by-frame movie-reader computer-image-analysis system with visual ratings [24]. Visual ratings were shown to be highly variable, and differences between objective and subjective methods and among laboratories were large.…”

Section: Introductionmentioning

confidence: 98%

Visual Versus Cinemicrographic Evaluation of Human Sperm Motility and Morphology

Freund

Oliveira

1987

Archives of Andrology

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Ratings of human sperm motility by visual estimation through the microscope remain important measures of semen quality and of male fertility. More objective methods, including cinemicrography, time lapse photography, and videomicrography, are advocated. Subjective (visual) and objective (cinemicrographic) ratings of motility were compared. Sixty workers in 30 laboratories rated motilities of 40 specimens on motion picture film, and motilities were also measured by cinephotomicrographic methods. The morphology of each of the motile and immotile sperm was rated. In 34 of 40 specimens visual ratings were higher (range = +2 to +31%) than actual percentage motility. Specimens with both high sperm concentration and forward progression received the highest overestimations by visual rating. This was especially apparent in specimens with the highest motility. There was a statistically significant positive relationship between sperm motility and morphology rated on a one-by-one basis, but the relationship was too small to influence the visual rating of human sperm motility.

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Movement characteristics of human spermatozoa analysed from kinemicrographs

Cited by 31 publications

References 4 publications

The movement of human spermatozoa in cervical mucus

The movement of human spermatozoa in cervical mucus

The Estimation of the Proportion of Motile Bull Spermatozoa in Various Diluents and a Comparison With the Proportion Eosinophilic

Visual Versus Cinemicrographic Evaluation of Human Sperm Motility and Morphology

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