The Estimation of the Proportion of Motile Bull Spermatozoa in Various Diluents and a Comparison With the Proportion Eosinophilic

Dott, H. M.

doi:10.1530/jrf.0.0450047

Cited by 13 publications

(11 citation statements)

References 12 publications

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“…The basic diluent used was a phosphate-buffered saline (PBS) solution, pH 7-3 (Dott, 1975 Dott & Foster (1972) and the proportion of eosinophilic spermatozoa was determined (100 counts/slide, 3 replicates/treatment) without clearing.…”

Section: Methodsmentioning

confidence: 99%

Motility and eosin uptake of formaldehyde-treated ram spermatozoa

Osinowo¹,

Bale²,

Oyedipe³

et al. 1982

Reproduction

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Section: Methodsmentioning

confidence: 99%

Motility and eosin uptake of formaldehyde-treated ram spermatozoa

Osinowo¹,

Bale²,

Oyedipe³

et al. 1982

Reproduction

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“…The underside of the plastic remained open to the air thus allowing oxygen to diffuse into the suspension. The slide was covered with a glass cover slip and placed on the stage of the Quantimet Image Averaging Computer (Dott 1975). The spermatozoa were illuminated with a dark ground condenser and two discriminator channels on the computer were set to count areas of different brightness.…”

Section: S{ierm Motility Measurementsmentioning

confidence: 99%

Chlorinated Sugars: A Biochemical Approach to the Control of Male Fertility

Ford

Waites

1978

Int J of Andrology

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6‐Chloro‐6‐deoxyglucose (120 μmol; 24 mg kg b.wt.), 6‐chloro‐6‐deoxyfructose (< 240 μ 48 mg/kg b.wt.), 6‐chloro‐6‐deoxysucrose (240 μmol; 85 mg/kg b.wt.), ′6‐chloro‐′6‐deoxysucrose (< 240 μmol; 85 mg/kg b.wt.) and 6.′6‐dichloro‐6,′6‐dideoxysucrose (240 μmol; 89 mg/kg b.wt.) administered to male rats daily, by oral gavage, render them infertile after 7 days treatment. The effect is sustained through at least 4 weeks of treatment and is reversed 3 to 6 weeks after treatment is withdrawn. Spermatozoa from the cauda epididymidis of treated rats oxidize only 10% as much glucose as spermatozoa from controls and their ATP and total adenine nucleotide content fall more rapidly during incubation with 2 mM [U14C]D‐glucose. These biochemical effects are associated with a more rapid decline in cell motility. The LD50 for 6‐chloro‐6‐deoxyglucose in the rat is > 16 g/kg b.wt. Administration of [36Cl] ′6‐chloro‐′6‐deoxysucrose has shown this compound to be extensively dechlorinated in vivo. Chlorodeoxy sugars are discussed as potential male antifertility agents worthy of further investigation.

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“…The mathematical derivation of the Equation 1 for mean speed from the probability after effect (Rothschild, 1953) (Dott, 1975). Thus, mean speed, total number density, and percentage motility, three primary physical parameters that characterize a suspension, could be simul¬ taneously and automatically determined.…”

Section: Introductionmentioning

confidence: 99%