Movement correction method for laser speckle contrast imaging of cerebral blood flow in cranial windows in rodents

Guilbert, Jérémie; Desjardins, Michèle

doi:10.1002/jbio.202100218

Cited by 13 publications

(9 citation statements)

References 38 publications

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“…In another study, they mounted the pair of laser-camera on a motorized stabilizer in order to suppress the amount of applied movements [26]. Recently, Guilbert et al [27] have regressed out the movement-related signal by identifying a static region in a speckle image. Liu et al proposed non-rigid registration of temporally averaged perfusion maps during which the imaging object moves.…”

Section: Related Workmentioning

confidence: 99%

Reliability of handheld laser speckle contrast perfusion imaging demonstrated in psoriasis lesions

Chizari¹,

Schaap

Knop

et al. 2022

Photonics in Dermatology and Plastic Surgery 2022

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We assessed the reliability of handheld laser speckle contrast perfusion imaging by evaluating mounted/handheld measurement pairs operated on psoriasis lesions in three steps. First, we made a denoised perfusion map per measurement based on spatial alignment of raw speckle frames and temporal averaging of perfusion frames. Second, we used the measured on-surface speed information to compensate the movement-induced perfusion by extrapolation of the local perfusion values to the value corresponds to zero on-surface speed. Third, we compared mounted/handheld measurement pairs based on perfusion inhomogeneity and increased perilesional perfusion criteria independent of the movement artefact compensation mentioned in the second step. We conclude that after proper post-processing, handheld LSCI measurements can be as reliable as mounted measurements in terms of geometrical distorting, but with challenges to be overcome for correcting perfusion values.

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Section: Related Workmentioning

confidence: 99%

Reliability of handheld laser speckle contrast perfusion imaging demonstrated in psoriasis lesions

Chizari¹,

Schaap

Knop

et al. 2022

Photonics in Dermatology and Plastic Surgery 2022

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“…Optical imaging approaches have been used to investigate structural and functional brain connections in rodents. For example, the optical neuroimaging methods such as two- or multi-photon excitation (2PE or MPE) imaging [ 26 - 29 ], calcium-sensitive dye imaging (calcium imaging, CaSDI) [ 30 , 31 ], voltage-sensitive dye imaging (VSDI) [ 32 - 34 ], laser speckle contrast imaging (LSCI) [ 35 - 38 ], optical intrinsic signal imaging (OISI) [ 35 , 39 - 42 ], and ultrasound imaging (USI) combined with light [ 43 , 44 ] have been widely used. These techniques enabled the investigation of vascular and cellular structures, neuronal activation, blood flow, blood pressure, and oxygen saturation.…”

Section: Optical Neuroimaging Technologiesmentioning

confidence: 99%

“…While the cerebrum has been a predominant research subject in neuroscience, research on the spinal cord, which bridges the brain and peripheral nerve, has become increasingly important with the increased focus on peripheral nerve disorders. Previously, imaging for lumbar [ 37 ] or thoracic [ 36 ] has been majorly shown, which is straightforward to access and significant in size even for a small mouse, but lately, cervical imaging has also been tried [ 38 ]. SCCW was used for long-term monitoring of brain cancer metastases through the blood-brain barrier (BBB) [ 37 , 38 ] or the response of astrocytes and microglias to ischemic brain injury [ 36 ].…”

Section: Diverse Cranial and Spinal Window Modelsmentioning

confidence: 99%

“…Previously, imaging for lumbar [ 37 ] or thoracic [ 36 ] has been majorly shown, which is straightforward to access and significant in size even for a small mouse, but lately, cervical imaging has also been tried [ 38 ]. SCCW was used for long-term monitoring of brain cancer metastases through the blood-brain barrier (BBB) [ 37 , 38 ] or the response of astrocytes and microglias to ischemic brain injury [ 36 ]. Spinal cord with SCCW was observed after spinal injury through microglia inflammation and heterogenous dieback of axon stumps [ 69 , 70 ].…”

Section: Diverse Cranial and Spinal Window Modelsmentioning

confidence: 99%

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Cranial and Spinal Window Preparation for in vivo Optical Neuroimaging in Rodents and Related Experimental Techniques

Yeon

Kim

et al. 2022

Exp Neurobiol

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Optical neuroimaging provides an effective neuroscience tool for multi-scale investigation of the neural structures and functions, ranging from molecular, cellular activities to the inter-regional connectivity assessment. Amongst experimental preparations, the implementation of an artificial window to the central nervous system (CNS) is primarily required for optical visualization of the CNS and associated brain activities through the opaque skin and bone. Either thinning down or removing portions of the skull or spine is necessary for unobstructed long-term in vivo observations, for which types of the cranial and spinal window and applied materials vary depending on the study objectives. As diversely useful, a window can be designed to accommodate other experimental methods such as electrophysiology or optogenetics. Moreover, auxiliary apparatuses would allow the recording in synchrony with behavior of large-scale brain connectivity signals across the CNS, such as olfactory bulb, cerebral cortex, cerebellum, and spinal cord. Such advancements in the cranial and spinal window have resulted in a paradigm shift in neuroscience, enabling in vivo investigation of the brain function and dysfunction at the microscopic, cellular level. This Review addresses the types and classifications of windows used in optical neuroimaging while describing how to perform in vivo studies using rodent models in combination with other experimental modalities during behavioral tests. The cranial and spinal window has enabled longitudinal examination of evolving neural mechanisms via in situ visualization of the brain. We expect transformable and multi-functional cranial and spinal windows to become commonplace in neuroscience laboratories, further facilitating advances in optical neuroimaging systems.

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“…The perfusion measured on the opaque surface was used either to choose a number of movement-artifact-free frames 21 or to be removed from the perfusion values measured on tissue as a compensation of MA. 22 – 25 Altogether, a fundamental model to explain the underlying reason but also to predict MA is missing.…”

Section: Introductionmentioning

confidence: 99%

Prediction of motion artifacts caused by translation in handheld laser speckle contrast imaging

et al. 2023

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. Significance In handheld laser speckle contrast imaging (LSCI), motion artifacts (MA) are inevitable. Suppression of MA leads to a valid and objective assessment of tissue perfusion in a wide range of medical applications including dermatology and burns. Our study shines light on the sources of these artifacts, which have not yet been explored. We propose a model based on optical Doppler effect to predict speckle contrast drop as an indication of MA. Aim We aim to theoretically model MA when an LSCI system measuring on static scattering media is subject to translational displacements. We validate the model using both simulation and experiments. This is the crucial first step toward creating robustness against MA. Approach Our model calculates optical Doppler shifts in order to predict intensity correlation function and contrast of the time-integrated intensity as functions of applied speed based on illumination and detection wavevectors. To validate the theoretical predictions, computer simulation of the dynamic speckles has been carried out. Then experiments are performed by both high-speed and low-framerate imaging. The employed samples for the experiments are a highly scattering matte surface and a Delrin plate of finite scattering level in which volume scattering occurs. Results An agreement has been found between theoretical prediction, simulation, and experimental results of both intensity correlation functions and speckle contrast. Coefficients in the proposed model have been linked to the physical parameters according to the experimental setups. Conclusions The proposed model provides a quantitative description of the influence of the types of illumination and media in the creation of MA. The accurate prediction of MA caused by translation based on Doppler shifts makes our model suitable to study the influence of rotation. Also the model can be extended for the case of dynamic media, such as live tissue.

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Movement correction method for laser speckle contrast imaging of cerebral blood flow in cranial windows in rodents

Cited by 13 publications

References 38 publications

Reliability of handheld laser speckle contrast perfusion imaging demonstrated in psoriasis lesions

Reliability of handheld laser speckle contrast perfusion imaging demonstrated in psoriasis lesions

Cranial and Spinal Window Preparation for in vivo Optical Neuroimaging in Rodents and Related Experimental Techniques

Prediction of motion artifacts caused by translation in handheld laser speckle contrast imaging

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